Date published: 2025-9-9
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Ubiquitin Antibody (A-5) - Western Blotting - Image 390067
泛素抗体 (A-5): sc-166553 HRP. 对 MCF7 (A), PC-12 (B), HeLa (C), Jurkat + LLnL (D) 和 HL-60 (E) 全细胞裂解液中泛素表达的直接 Western 印迹分析.
泛素抗体 (A-5): sc-166553 HRP. 对 MCF7 (A), PC-12 (B), HeLa (C), Jurkat + LLnL (D) 和 HL-60 (E) 全细胞裂解液中泛素表达的直接 Western 印迹分析.

Ubiquitin 抗体 (A-5): sc-166553

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说明书
  • Ubiquitin抗体(A-5)是小鼠单克隆IgG2b κ, 在99篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长Ubiquitin的氨基酸1-76
  • 抗-Ubiquitin 抗体 (A-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Ubiquitin and polyubiquitin
  • 抗Ubiquitin抗体（A-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ubiquitin (A-5): sc-166553的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Ubiquitin 抗体 (A-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ubiquitin 抗体 (A-5) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Ubiquitin抗体，包括Ub, Ub+1和USPL1

泛素抗体（A-5）是一种IgG2b κ型小鼠单克隆泛素抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC(P)）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的泛素和多聚泛素。泛素抗体（A-5）既有非偶联形式的抗泛素抗体，也有多种偶联形式的抗泛素抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。泛素（Ub）是已知在进化上最为保守的蛋白质之一。泛素的主要功能是通过将异常、外来和错误折叠的蛋白质靶向26S蛋白酶体进行降解，从而清除这些蛋白质。这种由76个氨基酸组成的小蛋白质可以通过泛素的羧基末端基团与受体蛋白质上的赖氨酸氨基基团之间的异肽键共价连接到细胞蛋白质上。为了进行蛋白质水解，必须组装泛素寡聚体。在蛋白质水解底物上常见的泛素链具有一个异肽桥，该桥位于一个泛素分子的赖氨酸48和相邻泛素分子的羧基末端之间。泛素还通过消除抑制性蛋白质（如IκB-α和p27）来调节信号转导级联反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ubiquitin 抗体 (A-5) 参考文献:

  1. 质膜蛋白的泛素化和内吞作用：泛素蛋白连接酶 Nedd4/Rsp5p 家族的作用。  |  Rotin, D., et al. 2000. J Membr Biol. 176: 1-17. PMID: 10882424
  2. 受体介导和液相内吞作用所需的Rsp5泛素蛋白连接酶的域。  |  Dunn, R. and Hicke, L. 2001. Mol Biol Cell. 12: 421-35. PMID: 11179425
  3. U box 蛋白是泛素蛋白连接酶的一个新家族。  |  Hatakeyama, S., et al. 2001. J Biol Chem. 276: 33111-20. PMID: 11435423
  4. 具有泛素蛋白连接酶活性的雄激素受体 N-末端相互作用蛋白的克隆和表征。  |  Beitel, LK., et al. 2002. J Mol Endocrinol. 29: 41-60. PMID: 12200228
  5. 参与病毒内化的腺病毒蛋白招募泛素-蛋白连接酶。  |  Galinier, R., et al. 2002. Biochemistry. 41: 14299-305. PMID: 12450395
  6. 具有单泛素化能力的 E3 泛素蛋白连接酶 Parkin 的帕金森病致病变体的生化分析。  |  Hampe, C., et al. 2006. Hum Mol Genet. 15: 2059-75. PMID: 16714300
  7. 泛素-蛋白连接酶在神经退行性疾病中的作用。  |  Ardley, HC. and Robinson, PA. 2004. Neurodegener Dis. 1: 71-87. PMID: 16908979
  8. 一种专一性泛素-蛋白连接酶，用于III型蛋白底物。  |  Heller, H. and Hershko, A. 1990. J Biol Chem. 265: 6532-5. PMID: 2324089
  9. 酿酒酵母泛素结合酶（Rad6）的C端在底物和泛素蛋白连接酶（E3-R）相互作用中的作用。  |  Raboy, B. and Kulka, RG. 1994. Eur J Biochem. 221: 247-51. PMID: 8168512
  10. 泛素蛋白连接酶E6相关蛋白（E6-AP）作为其自身的底物。  |  Nuber, U., et al. 1998. Eur J Biochem. 254: 643-9. PMID: 9688277

订购信息

产品名称产品编号规格价格数量收藏夹

Ubiquitin 抗体 (A-5)

sc-166553
200 µg/ml
RMB2377.00

Ubiquitin (A-5): m-IgGκ BP-HRP 套装

sc-521653
200 µg Ab, 40 µg BP
RMB2662.00

Ubiquitin (A-5): m-IgG2b BP-HRP 套装

sc-548947
200 µg Ab; 10 µg BP
RMB2662.00

Ubiquitin 抗体 (A-5) AC

sc-166553 AC
500 µg/ml, 25% agarose
RMB3129.00

Ubiquitin 抗体 (A-5) HRP

sc-166553 HRP
200 µg/ml
RMB2377.00

Ubiquitin 抗体 (A-5) FITC

sc-166553 FITC
200 µg/ml
RMB2482.00

Ubiquitin 抗体 (A-5) PE

sc-166553 PE
200 µg/ml
RMB2580.00

Ubiquitin 抗体 (A-5) Alexa Fluor® 488

sc-166553 AF488
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (A-5) Alexa Fluor® 546

sc-166553 AF546
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (A-5) Alexa Fluor® 594

sc-166553 AF594
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (A-5) Alexa Fluor® 647

sc-166553 AF647
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (A-5) Alexa Fluor® 680

sc-166553 AF680
200 µg/ml
RMB2685.00

Ubiquitin 抗体 (A-5) Alexa Fluor® 790

sc-166553 AF790
200 µg/ml
RMB2685.00

I would like to stain ubiquitin on renal sections by IHC. Which demasking conditions do you recommend for this antibody (EDTA, citrate, etc.) ?

Asked by: pierrelo
Thank you for your question. Our recommended method is as follows: Heat treatment: Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Tech Support Europe
Date published: 2024-01-04

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Ub Antibody (A-5): sc-166553?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 5 out of 5 by from Great!I bought this product. It's very easy to use. I recommend you to buy this product.
Date published: 2024-01-10
Rated 5 out of 5 by from Great antibody to detect ubiquitination of proteinWe used this antibody to detect the ubiquitination of test protein, it worked great! Highly recommended!
Date published: 2019-10-22
Rated 5 out of 5 by from Works for C2C12 cells in Western blotsWe tried this antibody on blots of C2C12 proteins and got strong double bands at approximately 10 kDa. We were expecting to see more signal from Ub that was attached to larger proteins, and did not observe this; however, the highest molecular weight on the membranes was about 50 kDa.
Date published: 2017-12-05
Rated 5 out of 5 by from Work in IHCGood staining with low background using IHC in mouse tissue
Date published: 2017-08-28
Rated 5 out of 5 by from worth that muchSatisfactory Western Blot data of Ub expression in Jurkat and HeLa whole cell lysate.
Date published: 2017-05-07
Rated 5 out of 5 by from Great antibody1:200 used detect human liver tissue extract, got bands very clear.
Date published: 2017-04-22
Rated 5 out of 5 by from Strong IHC and western blotThis product reacts well in western blot and IHC Applications
Date published: 2017-01-10
Rated 5 out of 5 by from Very Specifc Antibody, Perfect for IHCThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Very beautiful pictures form tissue staining.
Date published: 2016-12-29
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Ubiquitin 抗体 (A-5) is rated 4.9 out of 5 by 12.
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