Date published: 2025-9-8

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tuberin 抗体 (B-5): sc-271314

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说明书
  • tuberin 抗体 B-5 是小鼠单克隆 IgG1 κ,tuberin抗体, 在12篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的tuberin的N-terminus
  • tuberin 抗体 (B-5) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 tuberin
  • 抗tuberin抗体(B-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取tuberin (B-5): sc-271314免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是tuberin Antibody (B-5) for WB applications. 的首选辅助检测试剂,这些试剂现与tuberin Antibody (B-5) 打包提供(请参阅下面的订购信息)。

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tuberin 抗体(B-5)是一种 IgG1 κ 型鼠单克隆 tuberin 抗体(也称为结节性硬化症复合物(TSC)亚基2抗体、TSC复合物亚基2抗体或胞质hamartin伴侣抗体),可通过WB、IP、IF和ELISA方法检测人源tuberin蛋白。tuberin 抗体(B-5)既可以是非偶联的抗tuberin抗体形式,也可以是多种偶联形式的抗tuberin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。结节性硬化症复合物(TSC)是一种常染色体显性遗传性疾病,其特征是智力迟钝和称为错构瘤的独特肿瘤的广泛发展。已有两个不同的基因位点与TSC相关联;其中之一的位点,即结节性硬化症-2基因(TSC2),编码一种称为tuberin的蛋白质,另一个位点,即结节性硬化症-1基因(TSC1),编码一种称为hamartin的蛋白质。Tuberin和hamartin相互作用形成胞质复合物。Hamartin与ezrin-radixin-moesin(ERM)肌动蛋白结合蛋白家族相互作用,hamartin活性的抑制会导致细胞粘附的丧失。Hamartin存在于大多数成人组织中,在脑、心脏和肾脏中有强表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

tuberin 抗体 (B-5) 参考文献:

  1. 反义抑制 TSC1 基因产物 hamartin 可增强经 NGF 处理的 PC12h 细胞的神经元生长。  |  Floricel, F., et al. 2007. Brain Dev. 29: 502-9. PMID: 17376623
  2. Tuberin:一种受多种受体酪氨酸激酶和G蛋白偶联受体控制的、受刺激调节的肿瘤抑制蛋白。  |  Wu, EH., et al. Neurosignals. 15: 217-27. PMID: 17389815
  3. Krev-1 基因产物抑制 GTPase 活化蛋白对 Ras-p21 GTPase 的刺激。  |  Frech, M., et al. 1990. Science. 249: 169-71. PMID: 2164710
  4. 肾细胞肥大中的Tuberin。  |  Habib, SL. 2014. Cell Cycle. 13: 869-70. PMID: 24552810
  5. 与果蝇基因 Dras3 同源的人类 cDNA rap1 和 rap2 在 '效应区 '编码与 ras 密切相关的蛋白质。  |  Pizon, V., et al. 1988. Oncogene. 3: 201-4. PMID: 3045729
  6. 编码 ras 相关蛋白(rap1B)的人类 cDNA 的核苷酸序列。  |  Pizon, V., et al. 1988. Nucleic Acids Res. 16: 7719. PMID: 3137530
  7. 脑发育中细胞分化过程中的Tuberin水平。  |  Fidalgo da Silva, E., et al. 2023. Differentiation. 130: 43-50. PMID: 36608575
  8. 鉴定结节性硬化症-2 的产物 tuberin。Tuberin具有特异的Rap1GAP活性。  |  Wienecke, R., et al. 1995. J Biol Chem. 270: 16409-14. PMID: 7608212
  9. 小鼠的 tuberin 基因同源物(TSC2)映射到小鼠 17 号染色体和人类 16p13.3 之间的保守同源组。  |  Olsson, PG., et al. 1995. Genomics. 25: 339-40. PMID: 7774953
  10. 结节性硬化症的分子遗传学。  |  Sampson, JR. and Harris, PC. 1994. Hum Mol Genet. 3 Spec No: 1477-80. PMID: 7849741
  11. 调节 Ras 及其近亲的蛋白质。  |  Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
  12. 16 号染色体上结节性硬化症基因的鉴定和特征描述。  |  ,. 1993. Cell. 75: 1305-15. PMID: 8269512

订购信息

产品名称产品编号规格价格数量收藏夹

tuberin 抗体 (B-5)

sc-271314
200 µg/ml
RMB2377.00

tuberin (B-5): m-IgG Fc BP-HRP 套装

sc-527231
200 µg Ab; 10 µg BP
RMB2662.00

tuberin (B-5): m-IgG1 BP-HRP 套装

sc-532604
200 µg Ab; 20 µg BP
RMB2662.00

tuberin 抗体 (B-5) AC

sc-271314 AC
500 µg/ml, 25% agarose
RMB3129.00

tuberin 抗体 (B-5) HRP

sc-271314 HRP
200 µg/ml
RMB2377.00

tuberin 抗体 (B-5) FITC

sc-271314 FITC
200 µg/ml
RMB2482.00

tuberin 抗体 (B-5) PE

sc-271314 PE
200 µg/ml
RMB2580.00

tuberin 抗体 (B-5) Alexa Fluor® 488

sc-271314 AF488
200 µg/ml
RMB2685.00

tuberin 抗体 (B-5) Alexa Fluor® 546

sc-271314 AF546
200 µg/ml
RMB2685.00

tuberin 抗体 (B-5) Alexa Fluor® 594

sc-271314 AF594
200 µg/ml
RMB2685.00

tuberin 抗体 (B-5) Alexa Fluor® 647

sc-271314 AF647
200 µg/ml
RMB2685.00

tuberin 抗体 (B-5) Alexa Fluor® 680

sc-271314 AF680
200 µg/ml
RMB2685.00

tuberin 抗体 (B-5) Alexa Fluor® 790

sc-271314 AF790
200 µg/ml
RMB2685.00

What is the total protein amount should we use to detect by tuberin Antibody (B-5): sc-271314?

Asked by: THUY ANH
Thank you for your question. We recommend using 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane on an SDS gel.
Answered by: Tech Support Europe
Date published: 2024-04-10

Can tuberin (B-5): sc-271314 monoclonal antibody be used in Western blots with mouse tissue or cells? 

Asked by: IT416
SC-271314 is only validated for Human. We do not recommend this for mouse tissue or Cells.
Answered by: Technical Service
Date published: 2023-07-25

Can tuberin (B-5): sc-271314 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, tuberin (B-5): sc-271314 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 条带很清晰之前申请了试用装,抗体很好用,打算购买正式装,效果很好,抗体特异性良好,在目标蛋白区域无干扰杂带,棒!
Date published: 2018-06-27
Rated 5 out of 5 by from wonderfulI got this several monthes ago.unfortunately ,I used it a week ago,but I was happy that I got great results.thanks!
Date published: 2017-09-08
Rated 4 out of 5 by from usefulI tested this Ab to detect tsc2 expression in ovarian cancer cells from 4 different donors. There are some non-specific band around 200 kDa.
Date published: 2017-03-21
Rated 5 out of 5 by from robust bandsvery happy result , strong robust bands even at 1:1000 dilution for WB
Date published: 2017-02-11
Rated 5 out of 5 by from Our lab uses this antibody routinely andOur lab uses this antibody routinely and it works well each time in WB, strong band seen.
Date published: 2015-10-07
Rated 4 out of 5 by from Bands seen in WB using PCBands seen in WB using PC-3 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-06-05
Rated 5 out of 5 by from WB publishable data with human glioblastomaWB publishable data with human glioblastoma cell lines, T98G and LN-229 - SCBT Publication Review
Date published: 2013-01-17
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tuberin 抗体 (B-5) is rated 4.7 out of 5 by 7.
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