Date published: 2025-9-7
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TTK Antibody (D-8) - Western Blotting - Image 358403
TTK 抗体 (D-8): sc-376842. KNRK (A) 和 RPE-J (B) 全细胞裂解液中 TTK 表达的 Western 印迹分析.
TTK 抗体 (D-8): sc-376842. KNRK (A) 和 RPE-J (B) 全细胞裂解液中 TTK 表达的 Western 印迹分析.

TTK 抗体 (D-8): sc-376842

4.8(4)
寫評論提問

说明书
  • TTK 抗体 D-8 是小鼠单克隆 IgG1 κ，TTK抗体, 在1篇文献中引用，规格为200 µg/ml
  • 免疫human物种的TTK的氨基酸712-841
  • 推荐用于 mouse, rat 和 human 来源的TTK WB, IP, IF, IHC(P) 和 ELISA检测
  • 目前，我们还没有完成TTK 抗体 (D-8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

TTK抗体（D-8）是一种IgG1 κ小鼠单克隆TTK抗体（也称为TTK抗体），可通过WB、IP、IF、IHC(P)和ELISA检测来自小鼠、大鼠和人类的TTK蛋白。TTK抗体（D-8）既可以是非偶联的抗TTK抗体形式，也可以是多种偶联形式的抗TTK抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞周期的进行受到一系列蛋白激酶水平和活性变化以及其调节亚单位（即细胞周期蛋白）水平振荡的调节。通过用抗磷酸酪氨酸抗体筛选T细胞表达文库，克隆了具有人类起源的独特蛋白激酶TTK的全长序列。同样地，通过表达克隆从小鼠胚胎癌（EC）细胞系中分离出TTK的同源物。TTK/Esk是丝氨酸-苏氨酸/酪氨酸蛋白激酶家族的新成员，在多种增殖的人类细胞和组织中表达。在静止细胞和低增殖指数的细胞中，TTK-Esk表达降低或缺失。当细胞被诱导进入细胞周期时，TTK mRNA、蛋白质和激酶活性水平在细胞周期的G1到S阶段增加，并在G2到M阶段达到峰值，这表明TTK/Esk可能作为细胞周期调节组分发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TTK 抗体 (D-8) 参考文献:

  1. 多种编码 esk 激酶的 cDNA 预测了跨膜酶和细胞内酶的同工形式。  |  Douville, EM., et al. 1992. Mol Cell Biol. 12: 2681-9. PMID: 1375325
  2. 新型人类蛋白激酶 TTK 的表达与细胞增殖有关。  |  Mills, GB., et al. 1992. J Biol Chem. 267: 16000-6. PMID: 1639825
  3. TTK 可激活 Akt，促进肝癌细胞的增殖和迁移。  |  Liu, X., et al. 2015. Oncotarget. 6: 34309-20. PMID: 26418879
  4. TTK 是顺铂耐药卵巢癌的潜在治疗靶点。  |  Liu, Y., et al. 2021. J Ovarian Res. 14: 128. PMID: 34598710
  5. TTK 预测三阳性乳腺癌的预后，并调节肿瘤的增殖和侵袭。  |  Gao, YH., et al. 2022. Neoplasma. 69: 274-282. PMID: 34846157
  6. FOXM1在实体瘤中的预后价值：系统评价和荟萃分析。  |  Tang, Q., et al. 2023. Discov Med. 35: 208-220. PMID: 37272088
  7. 抑制 TTK 可激活 STING 信号，促进乳腺癌的抗 PD1 免疫疗法。  |  Hu, X., et al. 2024. Biochem Biophys Res Commun. 694: 149388. PMID: 38150917
  8. 为细胞周期中的 S 期和 M 期排序。  |  Nurse, P. 1994. Cell. 79: 547-50. PMID: 7954820
  9. G1 阶段的进展：根据提示循环。  |  Sherr, CJ. 1994. Cell. 79: 551-5. PMID: 7954821
  10. 过渡时期的有丝分裂  |  King, RW., et al. 1994. Cell. 79: 563-71. PMID: 7954823
  11. 细胞周期蛋白与癌症。二：细胞周期蛋白 D 和 CDK 抑制剂的时代到来。  |  Hunter, T. and Pines, J. 1994. Cell. 79: 573-82. PMID: 7954824
  12. 蛋白激酶 TTK 的细胞周期依赖性调控。  |  Hogg, D., et al. 1994. Oncogene. 9: 89-96. PMID: 8302607

订购信息

产品名称产品编号规格价格数量收藏夹

TTK 抗体 (D-8)

sc-376842
200 µg/ml
$316.00

What formulation would you recommend diluting the antibody in for Westerns and at what dilution?

Asked by: MA RA
Thank you for your question. We recommend using 5% nonfat milk + TBST (0.05% Tween-20) for blocking and incubating both primary and secondary antibodies for Western blotting. We recommend a starting dilution of 1:200 (range of 1:100-1:1,000).
Answered by: Technical Support
Date published: 2019-01-26

Would you recommend 5% BSA or milk powder to dilute the antibody for Western Blot?

Asked by: Geli Patho HD
Thank you for your question. Our recommended blocking reagent that we also use in our labs is UltraCruz® Blocking Reagent: sc-516214. If you are using dry milk powder or BSA instead, 5 % dry milk powder in TBS-T is generally better for antibodies that bind very prominently and produce a lot of unspecific binding and false positive western blot signals. Antibodies that do not bind as strongly are better incubated with 5 % BSA in TBS-T. Since BSA only contains one type of protein, its blocking effect is not as broad and is better than milk powder if the target protein is not expressed abundantly or the antibody only binds to its target with lower efficiency. It is also important to use only BSA and not milk powder when working with phospho-specific antibodies.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

Can this antibody be used for chromosome spreads (PFA)?

Asked by: mlamb
Thank you for your question. This antibody has not been validated specifically for chromosome spreads, however it did perform well in IF and IHC with PFA fixed cells and tissue sections. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-05-01

Can TTK (D-8): sc-376842 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, TTK (D-8): sc-376842 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from works well in WBIt detects the adquite bands with the right MW in WB in HCC cells lysates.
Date published: 2017-06-06
Rated 5 out of 5 by from Good monoclonal antibodystrong single band in WB in mouse Peritoneal macrophages,good specificity.
Date published: 2017-02-25
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-04-21
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TTK 抗体 (D-8) is rated 4.8 out of 5 by 4.
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