Date published: 2025-9-7
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TREX-1 Antibody (E-6) - Western Blotting - Image 371521
TREX-1 抗体 (E-6): sc-271870. HeLa (A) 和 Y79 (B) 全细胞裂解液中 TREX-1 表达的 Western 印迹分析.
TREX-1 抗体 (E-6): sc-271870. HeLa (A) 和 Y79 (B) 全细胞裂解液中 TREX-1 表达的 Western 印迹分析.

TREX-1 抗体 (E-6): sc-271870

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说明书
  • TREX-1 抗体 E-6 是小鼠单克隆 IgG2a κ，TREX-1抗体, 在11篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列290-369位于human物种的TREX-1的C-terminus
  • TREX-1 抗体 (E-6) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 three prime repair exonuclease 1
  • 抗TREX-1抗体（E-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-271870 X, 200 µg/0.1 ml)
  • 关于如何获取TREX-1 (E-6): sc-271870的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是TREX-1 Antibody (E-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与TREX-1 Antibody (E-6) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外TREX抗体，包括TREX-2

TREX-1抗体（E-6）是一种IgG2a κ型小鼠单克隆TREX-1抗体（也称为TREX1抗体），可通过WB、IP、IF和ELISA检测人源TREX-1蛋白。TREX-1抗体（E-6）既可以是非偶联的抗TREX-1抗体形式，也可以是多种偶联形式的抗TREX-1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。TREX-1（三引物修复核酸外切酶1），也称为滋养层表达1、CRV、AGS1、AGS5、DRN3、HERNS或DNase III，是核酸外切酶超家族的一员，属于TREX家族。TREX家族的成员参与DNA代谢和修复。TREX-1在脾、肝、胸腺、结肠、心脏、脑和小肠中表达。它定位于细胞核，并以同源二聚体的形式存在。TREX-1是一种核酸外切酶，其活性需要镁作为辅因子。TREX-1对双链DNA有偏好，并在3′-5′方向上起作用，产生核苷5′-磷酸。TREX-1还与SET复合物（一种内质网（ER）相关复合物）相互作用，并参与颗粒酶A介导的细胞凋亡。编码TREX-1的基因的突变或缺陷已与多种疾病相关，包括系统性红斑狼疮、冻疮性狼疮（CHBL）、Aicardi-Goutieres综合征1型（AGS1）和5型（AGS5）以及常染色体显性遗传性视网膜血管病变伴脑白质营养不良（CRV）。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TREX-1 抗体 (E-6) 参考文献:

  1. 人类 TREX 复合物在剪接过程中对 mRNA 的招募。  |  Masuda, S., et al. 2005. Genes Dev. 19: 1512-7. PMID: 15998806
  2. ATRIP 通过多种相互作用与复制蛋白 A 包覆的 ssDNA 结合。  |  Namiki, Y. and Zou, L. 2006. Proc Natl Acad Sci U S A. 103: 580-5. PMID: 16407120
  3. 错配修复蛋白是烷基化 DNA 损伤的传感器。  |  Wang, JY. and Edelmann, W. 2006. Cancer Cell. 9: 417-8. PMID: 16766259
  4. 外切酶 TREX1 是 SET 复合物中的一种，它与 NM23-H1 协同作用，在颗粒酶 A 介导的细胞死亡过程中降解 DNA。  |  Chowdhury, D., et al. 2006. Mol Cell. 23: 133-42. PMID: 16818237
  5. 编码 3'-5' DNA 外切酶 TREX1 的基因突变导致 AGS1 基因座上的艾卡迪-古蒂耶尔综合征。  |  Crow, YJ., et al. 2006. Nat Genet. 38: 917-20. PMID: 16845398
  6. 编码 3'-5' DNA 外切酶 TREX1 的基因突变与系统性红斑狼疮有关。  |  Lee-Kirsch, MA., et al. 2007. Nat Genet. 39: 1065-7. PMID: 17660818
  7. DNA 管理不善会导致免疫系统失灵。  |  Coscoy, L. and Raulet, DH. 2007. Cell. 131: 836-8. PMID: 18045527
  8. Trex1 外切酶降解 ssDNA，防止慢性检查点激活和自身免疫性疾病。  |  Yang, YG., et al. 2007. Cell. 131: 873-86. PMID: 18045533
  9. 与系统性红斑狼疮患者自身抗体相关的 TREX1 多态性。  |  Hur, JW., et al. 2008. Rheumatol Int. 28: 783-9. PMID: 18092167
  10. Trex1 可调控溶酶体生物生成和干扰素无关的抗病毒基因激活。  |  Hasan, M., et al. 2013. Nat Immunol. 14: 61-71. PMID: 23160154
  11. TREX1 在正常人脑中由小胶质细胞表达，在受缺血影响的区域则会增加。  |  Kothari, PH., et al. 2018. Brain Pathol. 28: 806-821. PMID: 30062819
  12. TREX1 细胞膜 DNA 降解与自身免疫性疾病和癌症免疫相关。  |  Fang, L., et al. 2023. Clin Exp Immunol. 211: 193-207. PMID: 36745566

订购信息

产品名称产品编号规格价格数量收藏夹

TREX-1 抗体 (E-6)

sc-271870
200 µg/ml
RMB2377.00

TREX-1 (E-6): m-IgG Fc BP-HRP 套装

sc-529277
200 µg Ab; 10 µg BP
RMB2662.00

TREX-1 (E-6): m-IgGκ BP-HRP 套装

sc-522086
200 µg Ab, 40 µg BP
RMB2662.00

TREX-1 (E-6): m-IgG2a BP-HRP 套装

sc-547289
200 µg Ab; 10 µg BP
RMB2662.00

TREX-1 抗体 (E-6) X

sc-271870 X
200 µg/0.1 ml
RMB2377.00

TREX-1 抗体 (E-6) AC

sc-271870 AC
500 µg/ml, 25% agarose
RMB3129.00

TREX-1 抗体 (E-6) HRP

sc-271870 HRP
200 µg/ml
RMB2377.00

TREX-1 抗体 (E-6) FITC

sc-271870 FITC
200 µg/ml
RMB2482.00

TREX-1 抗体 (E-6) PE

sc-271870 PE
200 µg/ml
RMB2580.00

TREX-1 抗体 (E-6) Alexa Fluor® 488

sc-271870 AF488
200 µg/ml
RMB2685.00

TREX-1 抗体 (E-6) Alexa Fluor® 546

sc-271870 AF546
200 µg/ml
RMB2685.00

TREX-1 抗体 (E-6) Alexa Fluor® 594

sc-271870 AF594
200 µg/ml
RMB2685.00

TREX-1 抗体 (E-6) Alexa Fluor® 647

sc-271870 AF647
200 µg/ml
RMB2685.00

TREX-1 抗体 (E-6) Alexa Fluor® 680

sc-271870 AF680
200 µg/ml
RMB2685.00

TREX-1 抗体 (E-6) Alexa Fluor® 790

sc-271870 AF790
200 µg/ml
RMB2685.00

我想申请免费小样

Asked by: Mary2019
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。如何获取免费试用装，请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-12-30

We detect an additional band at approximately 25kDa in 293T cells. Is there any information about what this band could be?

Asked by: julia1
Thank you for your question. We do not have any similar data using our TREX-1 Antibody (E-6): sc-271870 in 293T cells. According to Uniprot, there are 3 potential isoforms of TREX-1 predicted at 39, 33 and 32 kDa (http://www.uniprot.org/uniprot/Q9NSU2#sequences). We would recommend performing a secondary antibody control (omit primary antibody) to determine if the band you are observing is a result of non-specific binding of the secondary antibody alone.
Answered by: Technical Support
Date published: 2017-03-09

When should the TransCruz reagent sc-271870 X be used instead of the standard form sc-271870?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 14
Date published: 2017-02-07
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Rated 5 out of 5 by from Very good antibody for IFI used TREX-1 for IF, the antibody diluted at 1:100 and incubated 3h at room temperature. I used the secondary Goat anti mouse IgG (H+L) Alexa Fluor 568 at dilution 1:500 for 1h. I obtained a specific staining with little background noise.
Date published: 2021-04-30
Rated 5 out of 5 by from Excellent antibody for human TREX1Have used in 5% BSA and 5% milk at concentrations of 1:2000 with secondary concentrations of 1:4000 with excellent results. This antibody is specific for human TREX1 and will not cross react with mouse Trex1.
Date published: 2017-08-04
Rated 5 out of 5 by from Great for detection of mouse and rat TREX-1The (E-6) clone is the preferred choice for Western blot detection of mouse and rat TREX-1 (three prime repair exonuclease 1) as demonstrated by the extensive Western blot data in various cell lines.
Date published: 2017-01-23
Rated 5 out of 5 by from Produced nice Western blot data of TREXProduced nice Western blot data of TREX-1 expression in HCT-8 and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2014-04-22
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TREX-1 抗体 (E-6) is rated 5.0 out of 5 by 4.
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