Date published: 2025-9-8
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TRB-3 Antibody (D-4) - Western Blotting - Image 399421
TRB-3 抗体 (D-4) HRP: sc-365842 HRP. 直接对 Hep G2 全细胞裂解液中 TRB-3 的表达进行免疫印迹分析.
TRB-3 抗体 (D-4) HRP: sc-365842 HRP. 直接对 Hep G2 全细胞裂解液中 TRB-3 的表达进行免疫印迹分析.

TRB-3 抗体 (D-4): sc-365842

3.8(8)
寫評論提問

说明书
  • TRB-3 抗体 D-4 是小鼠单克隆 IgG1 κ，TRB-3抗体, 在12篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-165位于mouse物种的TRB-3的N-terminus
  • TRB-3 抗体 (D-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TRB-3
  • 抗TRB-3抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TRB-3 (D-4): sc-365842的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 TRB-3 抗体 (D-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TRB-3 抗体 (D-4) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 TRB 抗体 包括 TRB-1, TRB-2和TRB-3

TRB-3抗体（D-4）是一种IgG1 κ小鼠单克隆TRB-3抗体（也被称为Tribbles伪激酶3抗体、TRB3抗体、P65相互作用抑制剂NF-Kappa-B抗体、Tribbles同源物3抗体、C20orf97抗体、SKIP3抗体、NIPK抗体、SINK抗体、神经元细胞死亡诱导假定激酶抗体、P65相互作用抑制剂NF-KappaB抗体、染色体20开放阅读框97抗体或Tribbles同源物3（果蝇）抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的TRB-3蛋白。TRB-3抗体（D-4）既有非偶联抗TRB-3抗体形式，也有多种偶联形式的抗TRB-3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。TRB-3（tribbles 3），也被称为NIPK（神经元细胞死亡诱导蛋白激酶），通过直接结合Akt激酶并阻止其激活来破坏胰岛素信号传导。TRB3与ATF4结合，抑制其转录激活活性，并调节MAP激酶的激活。在肝脏中，TRB-3是PPAR-alpha的靶标。与野生型小鼠相比，糖尿病小鼠肝脏中TRB3 RNA和蛋白的含量更高。TRB3导致具有2型糖尿病易感性的个体产生胰岛素抵抗。TRB-3在肝脏、胰腺、外周血白细胞和骨髓中的表达量最高。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

TRB-3 抗体 (D-4)

sc-365842
200 µg/ml
RMB2377.00

TRB-3 (D-4): m-IgG Fc BP-HRP 套装

sc-529489
200 µg Ab; 10 µg BP
RMB2662.00

TRB-3 (D-4): m-IgGκ BP-HRP 套装

sc-522391
200 µg Ab, 40 µg BP
RMB2662.00

TRB-3 (D-4): m-IgG1 BP-HRP 套装

sc-543540
200 µg Ab; 20 µg BP
RMB2662.00

TRB-3 抗体 (D-4) AC

sc-365842 AC
500 µg/ml, 25% agarose
RMB3129.00

TRB-3 抗体 (D-4) HRP

sc-365842 HRP
200 µg/ml
RMB2377.00

TRB-3 抗体 (D-4) FITC

sc-365842 FITC
200 µg/ml
RMB2482.00

TRB-3 抗体 (D-4) PE

sc-365842 PE
200 µg/ml
RMB2580.00

TRB-3 抗体 (D-4) Alexa Fluor® 488

sc-365842 AF488
200 µg/ml
RMB2685.00

TRB-3 抗体 (D-4) Alexa Fluor® 546

sc-365842 AF546
200 µg/ml
RMB2685.00

TRB-3 抗体 (D-4) Alexa Fluor® 594

sc-365842 AF594
200 µg/ml
RMB2685.00

TRB-3 抗体 (D-4) Alexa Fluor® 647

sc-365842 AF647
200 µg/ml
RMB2685.00

TRB-3 抗体 (D-4) Alexa Fluor® 680

sc-365842 AF680
200 µg/ml
RMB2685.00

TRB-3 抗体 (D-4) Alexa Fluor® 790

sc-365842 AF790
200 µg/ml
RMB2685.00

I am using TRB-3 (D-4): sc-365842 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not workUse Tm(ER stress-inducer, 5ug/ml)-treated hepa1-6 cell samples, dilution to 1:500, not see any band in the right protein size
Date published: 2017-08-17
Rated 5 out of 5 by from perform in the wbasked the sample for the preliminary experiment，and the result is nice.
Date published: 2017-08-13
Rated 5 out of 5 by from The function of this TRB3 antibody is excellentI have bought the product a month ago, and the experiment results turned out to be very good. We were pleased to see the function of TRB3 antibody was stable and the price was very acceptable.
Date published: 2017-08-09
Rated 5 out of 5 by from Antibody performed very well in Western BlotThis is the first time experience with TRB-3 and was a sample test from Santa Cruz. This marker was used in Human muscle and adipose tissue that is part of a Diabetes study in conjunction with the TXNIP pathway. The antibody performed very well in a Western Blot application and yielded a product size of 45kDa as indicated by the company. One band was present with a fairly clean signal.
Date published: 2017-05-11
Rated 1 out of 5 by from I am sorry that the product I used did not work.S-D rat ,pulmonary artery smooth muscle cell. the product I applied for western blot test did not work.I used 1:100 and tried twice ,did not get the result.
Date published: 2016-12-27
Rated 4 out of 5 by from Good band seen in WB using Hep G2 whole cellGood band seen in WB using Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-06-28
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-29
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-03-07
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TRB-3 抗体 (D-4) is rated 3.8 out of 5 by 8.
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