Date published: 2025-9-8
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TRB-3 Antibody (B-2) - Western Blotting - Image 287591
TRB-3 抗体 (B-2): sc-390242. L8 (A) 和 KNRK (B) 全细胞裂解液中 TRB-3 表达的 Western 印迹分析.
TRB-3 抗体 (B-2): sc-390242. L8 (A) 和 KNRK (B) 全细胞裂解液中 TRB-3 表达的 Western 印迹分析.

TRB-3 抗体 (B-2): sc-390242

4.3(6)
寫評論提問

说明书
  • TRB-3抗体(B-2)是小鼠单克隆IgG2a λ, 在18篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的TRB-3的内部区域内的氨基酸51-89之间
  • 抗-TRB-3 抗体 (B-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 TRB-3
  • 抗TRB-3抗体（B-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TRB-3 (B-2): sc-390242的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是TRB-3 Antibody (B-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与TRB-3 Antibody (B-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外TRB抗体，包括TRB-1, TRB-2和TRB-3

TRB-3抗体（B-2）是一种IgG2a λ小鼠单克隆TRB-3抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的TRB-3。TRB-3抗体（B-2）既有非偶联抗TRB-3抗体形式，也有多种偶联形式的抗TRB-3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。TRB-3（也被称为tribbles 3或NIPK，即神经元细胞死亡诱导蛋白激酶）通过直接结合Akt激酶并阻断其激活来破坏胰岛素信号传导。TRB3与ATF4结合，抑制其转录激活活性，并调节MAP激酶的激活。在肝脏中，TRB-3是PPAR-alpha的靶标。与野生型小鼠相比，糖尿病小鼠肝脏中的TRB3 RNA和蛋白质含量更高。TRB3会导致具有2型糖尿病易感性的个体产生胰岛素抵抗。TRB-3在肝脏、胰腺、外周血白细胞和骨髓中的表达量最高。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TRB-3 抗体 (B-2) 参考文献:

  1. TRB3：一种抑制肝脏中胰岛素激活 Akt/PKB 的 tribbles 同源物。  |  Du, K., et al. 2003. Science. 300: 1574-7. PMID: 12791994
  2. PGC-1 通过 PPAR-α 依赖性诱导 TRB-3 促进肝脏的胰岛素抵抗。  |  Koo, SH., et al. 2004. Nat Med. 10: 530-4. PMID: 15107844
  3. 控制有丝分裂原活化蛋白激酶级联的蛋白质家族--人类 Tribbles。  |  Kiss-Toth, E., et al. 2004. J Biol Chem. 279: 42703-8. PMID: 15299019
  4. 缺乏证据证明 TRB3/NIPK 在原代肝细胞中作为 PKB 介导的胰岛素信号的阻抑剂所起的作用。  |  Iynedjian, PB. 2005. Biochem J. 386: 113-8. PMID: 15469416
  5. 法尼类固醇 X 受体对碳水化合物代谢的调节。  |  Stayrook, KR., et al. 2005. Endocrinology. 146: 984-91. PMID: 15564327
  6. 丙戊酸钠诱导的人类乳头细胞基因表达改变：使用丙戊酸钠与代谢副作用缔合作用的线索。  |  Wood, JR., et al. 2005. Physiol Genomics. 20: 233-43. PMID: 15598877
  7. TRB3是一种新型ER应激诱导基因，通过ATF4-CHOP途径诱导并参与细胞死亡。  |  Ohoka, N., et al. 2005. EMBO J. 24: 1243-55. PMID: 15775988
  8. 人类 NIPK（TRB3, SKIP3）基因在应激条件下的活化特征。  |  Ord, D. and Ord, T. 2005. Biochem Biophys Res Commun. 330: 210-8. PMID: 15781252
  9. 哺乳动物 Tribbles 同源物 TRB3 的功能性 Q84R 多态性与意大利高加索人的胰岛素抵抗及相关心血管风险有关。  |  Prudente, S., et al. 2005. Diabetes. 54: 2807-11. PMID: 16123373

订购信息

产品名称产品编号规格价格数量收藏夹

TRB-3 抗体 (B-2)

sc-390242
200 µg/ml
RMB2377.00

TRB-3 (B-2): m-IgG Fc BP-HRP 套装

sc-526136
200 µg Ab; 10 µg BP
RMB2662.00

TRB-3 (B-2): m-IgG2a BP-HRP 套装

sc-546511
200 µg Ab; 10 µg BP
RMB2662.00

TRB-3 抗体 (B-2) AC

sc-390242 AC
500 µg/ml, 25% agarose
RMB3129.00

TRB-3 抗体 (B-2) HRP

sc-390242 HRP
200 µg/ml
RMB2377.00

TRB-3 抗体 (B-2) FITC

sc-390242 FITC
200 µg/ml
RMB2482.00

TRB-3 抗体 (B-2) PE

sc-390242 PE
200 µg/ml
RMB2580.00

TRB-3 抗体 (B-2) Alexa Fluor® 488

sc-390242 AF488
200 µg/ml
RMB2685.00

TRB-3 抗体 (B-2) Alexa Fluor® 546

sc-390242 AF546
200 µg/ml
RMB2685.00

TRB-3 抗体 (B-2) Alexa Fluor® 594

sc-390242 AF594
200 µg/ml
RMB2685.00

TRB-3 抗体 (B-2) Alexa Fluor® 647

sc-390242 AF647
200 µg/ml
RMB2685.00

TRB-3 抗体 (B-2) Alexa Fluor® 680

sc-390242 AF680
200 µg/ml
RMB2685.00

TRB-3 抗体 (B-2) Alexa Fluor® 790

sc-390242 AF790
200 µg/ml
RMB2685.00

TRB-3 (B-2) 中和勝肽

sc-390242 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-390242 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good antibodyUsed 1:2000 on SK-N-AS cell lysates. A strong and clear band just below 50kDa marker. Some unspecific bands observed between 37kDa and 25kDa markers.
Date published: 2023-06-08
Rated 1 out of 5 by from It wasn't good in WBWe tried the antibody in WB at a concentration of 1:1000 in lysates of HCC cell lines but we couldn't see any band at the predicted molecular weight. It didn't work also using it as the first antibody tested in a new membrane.
Date published: 2022-10-12
Rated 5 out of 5 by from Very nice antibody with human tissue!This antibody worked very well with Human muscle and adipose tissue in Western Blot at 1:200 primary dilution and produced a nice clean band at 45kDa. It results in a more robust signal with human tissue than does TRB-3 sc-365842, although both antibodies performed very well with this application.
Date published: 2017-05-11
Rated 5 out of 5 by from Excellent!This antibody is great in Western blot analysis of TRB-3 expression in L8 and KNRK whole cell lysates.
Date published: 2017-01-24
Rated 5 out of 5 by from Nice Western Blot data of TRBNice Western Blot data of TRB-3 expression in L8 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-02-06
Rated 5 out of 5 by from Nice Western Blot data of TRBNice Western Blot data of TRB-3 expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2013-11-11
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TRB-3 抗体 (B-2) is rated 4.3 out of 5 by 6.
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