Date published: 2025-9-6
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TLR4 Antibody (HTA125) - Flow Cytometry - Image 8051
TLR4 抗体 (HTA125): sc-13593. 用生物素连接的 TLR4 抗体 (HTA125), 然后用 Avidin-FITC 染色的 U-937 细胞的间接 FCM 分析: sc-2865. 黑线直方图代表同型对照--正常小鼠 IgG: sc-2762.
TLR4 Antibody (HTA125) - Flow Cytometry - Image 8051
TLR4 抗体 (HTA125): sc-13593. 用生物素连接的 TLR4 抗体 (HTA125), 然后用 Avidin-FITC 染色的 U-937 细胞的间接 FCM 分析: sc-2865. 黑线直方图代表同型对照--正常小鼠 IgG: sc-2762.
TLR4 Antibody (HTA125) - Flow Cytometry - Image 148524
TLR4 抗体 (HTA125) FITC: sc-13593 FITC. THP-1 细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2a: sc-2856.
TLR4 Antibody (HTA125) - Immunohistochemistry - Image 156502
TLR4 抗体 (HTA125): sc-13593. 福尔马林固定, 石蜡包埋的人肾上腺组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.
TLR4 抗体 (HTA125): sc-13593. 用生物素连接的 TLR4 抗体 (HTA125), 然后用 Avidin-FITC 染色的 U-937 细胞的间接 FCM 分析: sc-2865. 黑线直方图代表同型对照--正常小鼠 IgG: sc-2762.

TLR4 抗体 (HTA125): sc-13593

4.7(11)
寫評論提問

说明书
  • TLR4抗体(HTA125)是小鼠单克隆IgG2a κ, 在80篇文献中引用，规格为200 µg/ml
  • 针对Toll样受体4（TLR4）
  • TLR4 抗体 (HTA125) 推荐用于IP, IF, IHC(P) 和 FCM，检测human 来源的 TLR4/MD-2 complex
  • 抗TLR4抗体（HTA125）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物，用于生物学研究，sc-13593 L，200 µg/0.1 ml
  • 关于如何获取TLR4 (HTA125): sc-13593的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是TLR4 Antibody (HTA125) 适用于 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与TLR4 Antibody (HTA125) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外TLR抗体，包括TLR1, TLR10, TLR2, TLR3, TLR4, TLR5, TLR7, TLR8和TLR9

TLR4抗体（HTA125）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测人源TLR4/MD-2复合物。TLR4 (HTA125) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。TLR4通过识别革兰氏阴性细菌的脂多糖（LPS）在先天免疫系统中发挥关键作用，从而触发信号级联，激活核因子κB（NFkB），并随后产生促炎细胞因子。该功能对于在感染期间启动有效的免疫反应并保持体内平衡至关重要。TLR4主要位于免疫细胞（如巨噬细胞和树突状细胞）的表面，在那里TLR4充当病原体检测的哨兵。TLR4的正常功能对于宿主应对感染的能力至关重要，TLR4信号的失调与多种炎症性疾病和病症有关，这凸显了TLR4在健康和疾病中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

TLR4 抗体 (HTA125)

sc-13593
200 µg/ml
RMB2377.00

TLR4 (HTA125): m-IgG Fc BP-HRP 套装

sc-525482
200 µg Ab; 10 µg BP
RMB2662.00

TLR4 (HTA125): m-IgG2a BP-HRP 套装

sc-546279
200 µg Ab; 10 µg BP
RMB2662.00

TLR4 抗体 (HTA125) L

sc-13593 L
200 µg/0.1 ml
RMB2377.00

TLR4 抗体 (HTA125) AC

sc-13593 AC
500 µg/ml, 25% agarose
RMB3129.00

TLR4 抗体 (HTA125) HRP

sc-13593 HRP
200 µg/ml
RMB2377.00

TLR4 抗体 (HTA125) FITC

sc-13593 FITC
200 µg/ml
RMB2482.00

TLR4 抗体 (HTA125) PE

sc-13593 PE
200 µg/ml
RMB2580.00

TLR4 抗体 (HTA125) Alexa Fluor® 488

sc-13593 AF488
200 µg/ml
RMB2685.00

TLR4 抗体 (HTA125) Alexa Fluor® 546

sc-13593 AF546
200 µg/ml
RMB2685.00

TLR4 抗体 (HTA125) Alexa Fluor® 594

sc-13593 AF594
200 µg/ml
RMB2685.00

TLR4 抗体 (HTA125) Alexa Fluor® 647

sc-13593 AF647
200 µg/ml
RMB2685.00

TLR4 抗体 (HTA125) Alexa Fluor® 680

sc-13593 AF680
200 µg/ml
RMB2685.00

TLR4 抗体 (HTA125) Alexa Fluor® 790

sc-13593 AF790
200 µg/ml
RMB2685.00

What is the possible pH for TLR4 marker in IHC?

Asked by: Thaitra
Thank you for your question. The pH is 7.
Answered by: BlakeJ
Date published: 2024-04-05

Do you recommend a positive control for this antibody?

Asked by: sarahusc
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-10-18

How can you vote for your own antibody? Where are the references for all your comments?

Asked by: PaulPandora
Thank you very much for your question. We currently list 36 product citations from peer-reviewed journals for this antibody. The product reviews and ratings on our website for this antibody are provided either by customers, screened from select product citations or provided by our laboratory staff using this product e.g. in routine QC applications. The reviews are marked accordingly i.e. by customer nick name, individual SCBT QC employees or SCBT Publication Review. We feel, that this type of information might be useful for existing or potential customers researching this product.
Answered by: Technical Support Europe
Date published: 2017-09-04

Is TLR4 (HTA125) suitable for use in Western blotting of mouse samples?

Asked by: cjMara
No. We do not recommend this antibody for use in WB or for mouse samples. We would recommend an alternative mouse monoclonal antibody, sc-293072: TLR4 (25) for this application.
Answered by: Technical Support 15
Date published: 2017-01-22
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Rated 3 out of 5 by from This antibody is a good with immunohistochemistryThis antibody works with immunohistochemistry with human tissue.
Date published: 2020-06-12
Rated 4 out of 5 by from Works well in WB applicationWe analyzed human and mice leukocytes samples by WB
Date published: 2019-03-27
Rated 5 out of 5 by from 效果很好之前买过抗体，跑出来结果很好，这次准备还要再订购几个抗体，不仅质量好，价格也相对优惠，给我的实验提供很多帮助
Date published: 2018-01-04
Rated 5 out of 5 by from Excellent TLR4 antibody!This antibody works great for detecting TLR4 by IHC and even flow cytometry. It is very specific and gives a great signal. It has been published with more than 30 times.
Date published: 2017-01-11
Rated 5 out of 5 by from Great IHC stainingIHC staining results are excellent; very pleased !!
Date published: 2017-01-05
Rated 5 out of 5 by from This antibody is very good quality in WB and IHCThis antibody is very good quality in WB and IHC.<br /> It can get very good result.I suggest WB:1:200 dilution. IHC:1:50 dilution.
Date published: 2015-11-24
Rated 5 out of 5 by from Works very well for flow cytometry with UWorks very well for flow cytometry with U-937 and THP-1 samples. -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by from Great IHC data with paraffinGreat IHC data with paraffin-embedded human adrenal gland tissue samples. -SCBT QC
Date published: 2015-03-20
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TLR4 抗体 (HTA125) is rated 4.7 out of 5 by 11.
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