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TFIID/TATA Binding Protein/TBP Antibody (1TBP22) - Western Blotting - Image 378515
TFIID 抗体 (1TBP22): sc-101550. Jurkat (A), K-562 (B) 和 SK-N-MC (C) 细胞核提取物及 AN3 CA 全细胞裂解液 (D) 中 TFIID 表达的 Western 印迹分析.
TFIID 抗体 (1TBP22): sc-101550. Jurkat (A), K-562 (B) 和 SK-N-MC (C) 细胞核提取物及 AN3 CA 全细胞裂解液 (D) 中 TFIID 表达的 Western 印迹分析.

TFIID/TATA Binding Protein/TBP 抗体 (1TBP22): sc-101550

4.3(3)
寫評論提問

说明书
  • TFIID/TATA Binding Protein/TBP抗体(1TBP22)是小鼠单克隆IgG1 (kappa轻链)，规格为200 µg/ml
  • 免疫human物种的TFIID的氨基酸55-99
  • 推荐用于 mouse, rat 和 human 来源的TFIID WB 和 IP检测
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是TFIID/TATA Binding Protein/TBP Antibody (1TBP22) for WB applications. 的首选辅助检测试剂，这些试剂现与TFIID/TATA Binding Protein/TBP Antibody (1TBP22) 打包提供（请参阅下面的订购信息）。

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    研究信息

    関連項目

    额外TFII抗体，包括TFII-I, TFIIA-α, TFIIA-β, TFIIA-γ, TFIIB, TFIID, TFIIE-α, TFIIE-β和TFIIF RAP 30

    在真核生物系统中，蛋白质编码基因的转录启动是一个复杂的过程，需要RNA聚合酶II和广泛的辅助转录因子家族。这些因子可以分为两大类：一类是所有Pol II基因转录所需的基础因子，包括TFIIA、TFIIB、TFIID、TFIIE、TFIIF和TFIIH；另一类是调控基因表达的序列特异性因子。基础转录因子和Pol II通过与核心启动子元件（如通常位于转录起始位点上游25-30个碱基对的TATA盒）相互作用，在转录起始位点附近形成一个特定的多蛋白复合物。TFIID与TATA元件的结合会引发其他因子组装成一个启动前复合物。来自高等真核生物的TFIID的TATA结合亚基（被指定为TFIIDt或TBP）包含一个高度保守的180个氨基酸的C端结构域。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    TFIID/TATA Binding Protein/TBP 抗体 (1TBP22)

    sc-101550
    200 µg/ml
    		RMB2377.00

    TFIID/TATA Binding Protein/TBP (1TBP22): m-IgG Fc BP-HRP 套装

    sc-539782
    200 µg Ab; 10 µg BP
    		RMB2662.00

    TFIID/TATA Binding Protein/TBP (1TBP22): m-IgG1 BP-HRP 套装

    sc-541704
    200 µg Ab; 20 µg BP
    		RMB2662.00

    What is the recommended starting dilution for Western blot for TFIID (1TBP22): sc-101550 monoclonal antibody?

    Asked by: Cweed
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-101550.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 4 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human TFIID protein via Western Blot analysis. -SCBT QC
    Date published: 2015-07-03
    Rated 4 out of 5 by from An increase in signal was observed via WesternAn increase in signal was observed via Western Blot when comparing mouse TFIID transfected 293T cells with those that have not been transfected. -SCBT QC
    Date published: 2015-05-30
    Rated 5 out of 5 by from Clean blot with single band at expected sizeClean blot with single band at expected size in F9 and HeLa whole cell lysate. -SCBT QC
    Date published: 2014-11-15
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    TFIID/TATA Binding Protein/TBP 抗体 (1TBP22) is rated 4.3 out of 5 by 3.
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