Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
TFIID/TATA Binding Protein/TBP Antibody (58C9) - Western Blotting - Image 374816
TFIID/TATA 结合蛋白/TBP 抗体 (58C9): sc-421. NIH/3T3 (A), Sol8 (B) 和 C2C12 (C) 全细胞裂解液中 TFIID 表达的 Western 印迹分析.
TFIID/TATA 结合蛋白/TBP 抗体 (58C9): sc-421. NIH/3T3 (A), Sol8 (B) 和 C2C12 (C) 全细胞裂解液中 TFIID 表达的 Western 印迹分析.

TFIID/TATA Binding Protein/TBP 抗体 (58C9): sc-421

4.9(14)
寫評論提問

说明书
  • TFIID/TATA Binding Protein/TBP 抗体 58C9 是小鼠单克隆 IgG2b，TFIID/TATA Binding Protein/TBP抗体, 在82篇文献中引用，规格为200 µg/ml
  • 针对细菌产生的dTBP
  • TFIID/TATA Binding Protein/TBP 抗体 (58C9) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 TATA binding protein (TBP)
  • 抗TFIID/TATA Binding Protein/TBP抗体（58C9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-421 X, 200 µg/0.1 ml)
  • 关于如何获取TFIID/TATA Binding Protein/TBP (58C9): sc-421的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成TFIID/TATA Binding Protein/TBP 抗体 (58C9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 TFII 抗体 包括 TFII-I, TFIIA-α, TFIIA-β, TFIIA-γ, TFIIB, TFIID, TFIIE-α, TFIIE-β和TFIIF RAP 30

TFIID [TBP] 抗体（58C9）是一种小鼠单克隆 IgG2b 抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠和人源 TFIID 蛋白。TFIID [TBP] (58C9) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。TFIID 蛋白在真核细胞转录的启动中起着至关重要的作用，是组装在核心启动子区域的转录机制的关键组成部分。TFIID识别并结合TATA盒，TATA盒是位于转录起始位点上游约25-30个碱基对处的保守DNA序列，对于RNA聚合酶II和其他转录因子的招募至关重要。TATA结合蛋白（TBP）是TFIID的一个亚基，包含一个高度保守的C端结构域，该结构域对于功能至关重要，因为TBP有助于DNA弯曲，从而允许转录前启动复合物的正确组装。这一过程对于蛋白质编码基因的精确表达至关重要，使TFIID成为基因调控和细胞功能中不可或缺的角色。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TFIID/TATA Binding Protein/TBP 抗体 (58C9) 参考文献:

  1. TFIIA 通过与基本重复序列相互作用，诱导 TFIID 发生构象变化。  |  Lee, DK., et al. 1992. Mol Cell Biol. 12: 5189-96. PMID: 1406690
  2. 鉴定人类 TFIID 成分以及 250 kDa 多肽与 TATA 盒结合蛋白（TFIID tau）之间的直接相互作用。  |  Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
  3. 转录因子在控制人脑内皮转铁蛋白受体表达中的作用。  |  Holloway, K., et al. 2007. J Mol Biol. 365: 1271-84. PMID: 17125792
  4. TFIID 的人类 TAF5 亚基的结构分析和二聚化潜力。  |  Bhattacharya, S., et al. 2007. Proc Natl Acad Sci U S A. 104: 1189-94. PMID: 17227857
  5. 鉴定小型 TAF 复合物及其在组装含 TAF 复合物中的作用。  |  Demény, MA., et al. 2007. PLoS One. 2: e316. PMID: 17375202
  6. 酵母和阴沟肠杆菌 TAF(II)5 N 端结构域的晶体结构, 生化和遗传特征：对 TFIID 组装的影响。  |  Romier, C., et al. 2007. J Mol Biol. 368: 1292-306. PMID: 17397863
  7. 管理转录复合体组装的 TATA 结合蛋白调控网络。  |  Huisinga, KL. and Pugh, BF. 2007. Genome Biol. 8: R46. PMID: 17407552
  8. 人类通用转录因子 IIE 的结构和功能特性。  |  Peterson, MG., et al. 1991. Nature. 354: 369-73. PMID: 1956398
  9. 哺乳动物 RNA 聚合酶 II 参与特异性转录的因子：转录因子 IIA, IID 和 IIB 在转录功能复合体形成过程中的作用。  |  Maldonado, E., et al. 1990. Mol Cell Biol. 10: 6335-47. PMID: 2247058
  10. 组蛋白羟化是一种可增强TFIID与H3K4me3结合的易化修饰。  |  Farrelly, LA., et al. 2019. Nature. 567: 535-539. PMID: 30867594
  11. RNA聚合酶II转录与部分组装的TFIID复合物。  |  Hisler, V., et al. 2023. bioRxiv.. PMID: 38076793
  12. TFIID复合物中的TAF2可调节部分蛋白编码基因的表达。  |  Cheng, IH., et al. 2024. Cell Death Discov. 10: 244. PMID: 38773077

订购信息

产品名称产品编号规格价格数量收藏夹

TFIID/TATA Binding Protein/TBP 抗体 (58C9)

sc-421
200 µg/ml
RMB2377.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) X

sc-421 X
200 µg/0.1 ml
RMB2377.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) AC

sc-421 AC
500 µg/ml, 25% agarose
RMB3129.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) HRP

sc-421 HRP
200 µg/ml
RMB2377.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) FITC

sc-421 FITC
200 µg/ml
RMB2482.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) PE

sc-421 PE
200 µg/ml
RMB2580.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) Alexa Fluor® 488

sc-421 AF488
200 µg/ml
RMB2685.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) Alexa Fluor® 546

sc-421 AF546
200 µg/ml
RMB2685.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) Alexa Fluor® 594

sc-421 AF594
200 µg/ml
RMB2685.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) Alexa Fluor® 647

sc-421 AF647
200 µg/ml
RMB2685.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) Alexa Fluor® 680

sc-421 AF680
200 µg/ml
RMB2685.00

TFIID/TATA Binding Protein/TBP 抗体 (58C9) Alexa Fluor® 790

sc-421 AF790
200 µg/ml
RMB2685.00

I would like to use this antibody for EMSA (Gelshift), would you recommend using the regular form or the x-form of the antibody (TransCruz reagent)?

Asked by: TinTin
We recommend using the TransCruz reagent form, sc-421 X, when performing ChIP or EMSA assays. This more concetrated form lets you use a smaller volume, which is desired when doing EMSA experiments. The x-form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 7
Date published: 2017-01-22
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_421, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Recommended for western blotting!It detected endogenous TBP in lysates from HEK 293T cells (1:500 dilution in 5% milk in TBS-0.1% Tween-20, overnight at 4C).
Date published: 2018-06-18
Rated 4 out of 5 by from Good for WB but weak for ChIPThis antibody is good for Western blot but only produces weak enrichment for ChIP.
Date published: 2018-05-19
Rated 5 out of 5 by from Works for ChIP!The 58C9 clone is published for ChIP. See PMID: 12612078 & 22441827 as examples.
Date published: 2016-12-22
Rated 5 out of 5 by from It has been reported that this antibody isIt has been reported that this antibody is suitable for use in EMSA applications using human samples. -SCBT Publication Review
Date published: 2015-06-22
Rated 5 out of 5 by from Publishable data obtained by ChIP using humanPublishable data obtained by ChIP using human HeLa cells. -SCBT Publication Review
Date published: 2015-05-25
Rated 5 out of 5 by from Positive detection of endogenously expressedPositive detection of endogenously expressed TFIID protein in NIH/3T3, HeLa and F9 cells through analysis by Western Blot. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from An increase in signal was observed via WesternAn increase in signal was observed via Western Blot when comparing mouse TFIID transfected 293T cells with those that have not been transfected. -SCBT QC
Date published: 2015-05-22
Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human TFIID protein via Western Blot analysis. -SCBT QC
Date published: 2015-05-18
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_zh_CN, sid_421, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 12ms
  • REVIEWS, PRODUCT
TFIID/TATA Binding Protein/TBP 抗体 (58C9) is rated 4.9 out of 5 by 14.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_zh_CN, sid_421, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 105ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。