Date published: 2025-9-6
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TFEB Antibody (C-6) - Western Blotting - Image 317970
TFEB 抗体 (C-6): sc-166736. Western 印迹分析 HeLa 全细胞裂解液中 TFEB 的表达.
TFEB 抗体 (C-6): sc-166736. Western 印迹分析 HeLa 全细胞裂解液中 TFEB 的表达.

TFEB 抗体 (C-6): sc-166736

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说明书
  • TFEB 抗体 C-6 是小鼠单克隆 IgG1 κ，TFEB抗体, 在28篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的TFEB的内部区域内的氨基酸455-470之间
  • TFEB 抗体 (C-6) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 TFEB
  • 抗TFEB抗体（C-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-166736 X, 200 µg/0.1 ml)
  • 关于如何获取TFEB (C-6): sc-166736的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是TFEB Antibody (C-6) for WB applications. 的首选辅助检测试剂，这些试剂现与TFEB Antibody (C-6) 打包提供（请参阅下面的订购信息）。

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基因信息
说明书与实验方案
研究信息

関連項目

额外TFE抗体，包括TFEC

TFEB抗体（C-6）是一种IgG1 κ小鼠单克隆TFEB抗体（也被称为E类基础螺旋-环-螺旋蛋白35抗体、BHLHE35抗体、TCFEB抗体、T细胞转录因子EB抗体或αTFEB抗体），它可以通过WB、IP、IF和ELISA等方法检测来自人类的TFEB蛋白。TFEB抗体（C-6）既有非偶联的抗TFEB抗体形式，也有多种偶联形式的抗TFEB抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。DNA结合因子TFE3包含相邻的螺旋-环-螺旋（HLH）和亮氨酸拉链（LZ）结构域，这些结构域被上游的基本区域所包围。这些蛋白质模体在其他转录因子中经常观察到，并且在Myc家族成员中特别常见。TFE3普遍表达，并且可以作为同二聚体或与两个相关蛋白质TFEB或TFEC形成的异二聚体直接与DNA结合。TFE3结合并激活免疫球蛋白重链增强子的microE3模体，以诱导B细胞特异性基因转录和DNA重组。TFEB与腺病毒的主要晚期启动子结合，并以同二聚体和与TFE3形成的异二聚体的形式特异性地与DNA结合。TFEB在胚胎中的表达水平较低，但在胎盘的滋养层细胞中表达水平较高，在胎盘的正常血管化过程中起着关键作用。TFEC与TFE3和密切相关蛋白小眼相关转录因子（MITF）共享bHLH/LZ结构，后者对黑色素细胞的分化至关重要。与TFE3不同，TFEC的表达在很大程度上仅限于成纤维细胞、肌细胞、软骨肉瘤细胞和骨髓瘤细胞。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TFEB 抗体 (C-6) 参考文献:

  1. 小鼠 bHLH-ZIP 转录因子 TFEC 和 TFEB 基因的克隆和表征揭示了所有 MiT 亚家族成员的共同基因组织。  |  Rehli, M., et al. 1999. Genomics. 56: 111-20. PMID: 10036191
  2. TFEB 具有独特的螺旋-环-螺旋/亮氨酸-拉链家族的 DNA 结合和寡聚特性。  |  Fisher, DE., et al. 1991. Genes Dev. 5: 2342-52. PMID: 1748288
  3. Myc 蛋白的 DNA 序列特异性结合。  |  Kerkhoff, E., et al. 1991. Proc Natl Acad Sci U S A. 88: 4323-7. PMID: 1827916
  4. TFE3：一种通过免疫球蛋白增强子 muE3 motif 激活转录的螺旋环螺旋蛋白。  |  Beckmann, H., et al. 1990. Genes Dev. 4: 167-79. PMID: 2338243
  5. TFEB 和 TFE3 的转录激活需要 AMPK 依赖性磷酸化。  |  Paquette, M., et al. 2021. Autophagy. 17: 3957-3975. PMID: 33734022
  6. TFEB依赖性溶酶体生物发生是衰老所必需的。  |  Curnock, R., et al. 2023. EMBO J. 42: e111241. PMID: 36970883
  7. TFEB是衰老过程和与年龄相关疾病的核心调节因子。  |  Abokyi, S., et al. 2023. Ageing Res Rev. 89: 101985. PMID: 37321382
  8. 转录因子EB（TFEB）在健康和疾病中的翻译后调控。  |  Takla, M., et al. 2023. EMBO Rep. 24: e57574. PMID: 37728021
  9. TFE3 的基本螺旋-环-螺旋-拉链结构域介导增强子与启动子之间的相互作用。  |  Artandi, SE., et al. 1994. Mol Cell Biol. 14: 7704-16. PMID: 7969114
  10. 编码人类 TFEC 同工型（一种新发现的转录调节因子）cDNA 的分子克隆。  |  Yasumoto, K. and Shibahara, S. 1997. Biochim Biophys Acta. 1353: 23-31. PMID: 9256061
  11. bHLH-Zip 转录因子 Tfeb 对胎盘血管化至关重要。  |  Steingrímsson, E., et al. 1998. Development. 125: 4607-16. PMID: 9806910
  12. TFEC 是基本螺旋-环-螺旋亮氨酸拉链转录因子小眼球-TFE 亚家族中一个受巨噬细胞限制的成员。  |  Rehli, M., et al. 1999. J Immunol. 162: 1559-65. PMID: 9973413

订购信息

产品名称产品编号规格价格数量收藏夹

TFEB 抗体 (C-6)

sc-166736
200 µg/ml
RMB2377.00

TFEB (C-6): m-IgG Fc BP-HRP 套装

sc-527181
200 µg Ab; 10 µg BP
RMB2662.00

TFEB (C-6): m-IgG1 BP-HRP 套装

sc-532554
200 µg Ab; 20 µg BP
RMB2662.00

TFEB 抗体 (C-6) X

sc-166736 X
200 µg/0.1 ml
RMB2377.00

TFEB 抗体 (C-6) AC

sc-166736 AC
500 µg/ml, 25% agarose
RMB3129.00

TFEB 抗体 (C-6) HRP

sc-166736 HRP
200 µg/ml
RMB2377.00

TFEB 抗体 (C-6) FITC

sc-166736 FITC
200 µg/ml
RMB2482.00

TFEB 抗体 (C-6) PE

sc-166736 PE
200 µg/ml
RMB2580.00

TFEB 抗体 (C-6) Alexa Fluor® 488

sc-166736 AF488
200 µg/ml
RMB2685.00

TFEB 抗体 (C-6) Alexa Fluor® 546

sc-166736 AF546
200 µg/ml
RMB2685.00

TFEB 抗体 (C-6) Alexa Fluor® 594

sc-166736 AF594
200 µg/ml
RMB2685.00

TFEB 抗体 (C-6) Alexa Fluor® 647

sc-166736 AF647
200 µg/ml
RMB2685.00

TFEB 抗体 (C-6) Alexa Fluor® 680

sc-166736 AF680
200 µg/ml
RMB2685.00

TFEB 抗体 (C-6) Alexa Fluor® 790

sc-166736 AF790
200 µg/ml
RMB2685.00

TFEB (C-6) 中和勝肽

sc-166736 P
100 µg/0.5 ml
RMB511.00

Bonjour , Je souhaiterais savoir si cet anticorps anti human TFEB #sc-166736 reconnait la souris ? D'avance merci Mme Cabirol

Asked by: Sand
Merci pour votre question. sc-166736 n'est pas recommandé pour être utilisé avec des échantillons de souris
Answered by: Tech Support Europe
Date published: 2022-07-01

Does the body work in other species as well? (mouse brain immunofluorescence)

Asked by: MonikaS
Thank you for your question. sc-166736 is just recommended for the detection of TFEB of human origin.
Answered by: Tech Support Europe
Date published: 2022-03-17

是否可以用于大鼠的组织或细胞

Asked by: pearsun
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据，以解答您物种反应性的相关问题。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-02-19

What application is the blocking peptide sc-166736 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good product!Lysates from Jurkat T cells and human CD8+ blasts were incubated with TFEB from Santa Cruz (sc-166736) diluted 1:500 in PBS 0,02% Tween20 for 3 hours at room temperature. The antibody gives a good signal at the proper molecular weight. A long incubation (at least 3 hours + long exposition during acquisition) may be required, as the signal is weak, but the specificity seems to be acceptable.
Date published: 2021-10-12
Rated 5 out of 5 by from AntibodyThis antibody is great, works well with western blots. Highly recommend.
Date published: 2020-10-27
Rated 3 out of 5 by from TFEB C6 sampleantibody tested with 1/500 dilution and o/n 4°C. gave a signal of correct size in HeLa whole cell lysate, but did not work for HCT8 cell line.
Date published: 2018-02-16
Rated 5 out of 5 by from 效果不错我用了TFEB（C-6）试用小样，免疫组化标记的结果比较特异，t（6；11）染色体易位石蜡包埋组织细胞核阳性。
Date published: 2017-10-25
Rated 4 out of 5 by from GOOD PICTUREI get a free sample. Chicken brain tissue , 1:200, NC, after several adjustment on protein sample , get the target line and no other lines.
Date published: 2017-09-26
Rated 5 out of 5 by from specific bandGood detection for endogenous detection of TFEB by WB (1:800, 5% BSA,TBST),we got specific band, no background signal,the condition should be optimized.
Date published: 2017-09-02
Rated 4 out of 5 by from Works on overexpressed/endogenous TFEB (WB, 293A)Works on overexpressed/endogenous TFEB (WB, 293A). RIPA extracted protein post 1-6 h serum starvation, weak endogenous band at ~70 kDa. 25 ug spl, LI-COR detection
Date published: 2017-04-04
Rated 5 out of 5 by from Highly recommendThis antibody is great! Nice solid bands on Western blot, specific IF staining results, too. Highly recommend .
Date published: 2017-03-10
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TFEB 抗体 (C-6) is rated 4.6 out of 5 by 13.
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