Date published: 2025-9-8

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Tau 抗体 (E-4): sc-515539

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说明书
  • Tau抗体(E-4)是小鼠单克隆IgG2b κ, 在2篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Tau (CNS isoform)的C-terminus的氨基酸358-379之间
  • 抗-Tau 抗体 (E-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 multiple Tau isoforms
  • 抗Tau抗体(E-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tau (E-4): sc-515539免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Tau 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Tau 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Tau抗体(E-4)是一种小鼠单克隆IgG2b kappa轻链抗体,用于检测Tau蛋白,也称为MAPT(微管相关蛋白Tau)或TAU。抗Tau抗体(E-4)可识别人类Tau的C末端氨基酸358-379之间的表位,确保通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)有效检测小鼠、大鼠和人类来源的Tau同种型。Tau单克隆抗体(E-4)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®变体,为各种实验应用提供了多功能性。Tau蛋白是一种长758个氨基酸的蛋白质,主要位于神经细胞的细胞质、细胞骨架和细胞膜中,在促进微管组装和稳定方面发挥着重要作用。这种定位对于维持神经元结构、促进细胞内运输和支持神经元极性至关重要,而神经元极性对于神经元的正常功能和通信至关重要。Tau与GSK-3β等激酶和PP2A等磷酸酶等关键蛋白相互作用,这些蛋白调节Tau的磷酸化状态和功能活性。Tau的正常功能对于细胞骨架的完整性和神经元的健康至关重要,而Tau的失调和聚集与神经退行性疾病的发展有关。异常的Tau积累和过度磷酸化是阿尔茨海默氏病、皮质基底变性(CBD)、进行性核上性麻痹(PSP)和其他tau蛋白病的主要特征,这使得Tau(E-4)抗体成为研究人员研究这些病症的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Tau 抗体 (E-4)

sc-515539
200 µg/ml
RMB2377.00

Tau (E-4): m-IgG Fc BP-HRP 套装

sc-531403
200 µg Ab; 10 µg BP
RMB2662.00

Tau (E-4): m-IgGκ BP-HRP 套装

sc-525086
200 µg Ab, 40 µg BP
RMB2662.00

Tau (E-4): m-IgG2b BP-HRP 套装

sc-550284
200 µg Ab; 10 µg BP
RMB2662.00

Tau 抗体 (E-4) AC

sc-515539 AC
500 µg/ml, 25% agarose
RMB3129.00

Tau 抗体 (E-4) HRP

sc-515539 HRP
200 µg/ml
RMB2377.00

Tau 抗体 (E-4) FITC

sc-515539 FITC
200 µg/ml
RMB2482.00

Tau 抗体 (E-4) PE

sc-515539 PE
200 µg/ml
RMB2580.00

Tau 抗体 (E-4) Alexa Fluor® 488

sc-515539 AF488
200 µg/ml
RMB2685.00

Tau 抗体 (E-4) Alexa Fluor® 546

sc-515539 AF546
200 µg/ml
RMB2685.00

Tau 抗体 (E-4) Alexa Fluor® 594

sc-515539 AF594
200 µg/ml
RMB2685.00

Tau 抗体 (E-4) Alexa Fluor® 647

sc-515539 AF647
200 µg/ml
RMB2685.00

Tau 抗体 (E-4) Alexa Fluor® 680

sc-515539 AF680
200 µg/ml
RMB2685.00

Tau 抗体 (E-4) Alexa Fluor® 790

sc-515539 AF790
200 µg/ml
RMB2685.00

Tau (E-4) 中和勝肽

sc-515539 P
100 µg/0.5 ml
RMB511.00

Hi, I am trying to figure out the epitope of Tau Antibody (E-4): sc-515539, the description says the epitope is between amino acids 358-379, but there are differnet isoforms. Can you send me the sequence of 358-379 (or the sc-515539 P seq)

Asked by: stefan stamm
Thank you for your question. The exact peptide sequence used as an antigen is considered proprietary information. However, we do publish that this is specific for an epitope mapping between amino acids 358-379 at the C-terminus of Tau (CNS isoform) of human origin. As a reference for the exact amino acids for this sequence, please refer to protein accession number NP_058518.1 on NCBI.
Answered by: Technical Support
Date published: 2019-01-26

What application is the blocking peptide sc-515539 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent!It beautifully works very well in IF of mouse primary neurons!
Date published: 2025-01-27
Rated 3 out of 5 by from Good Ab but not for Elisa coatingI got this Ab trial size for my Sandwich Elisa, this is good Ab but not for the Elisa coating AB at 1:500 dilution. Maybe I need less dilution, fortunately other Ab from Santa Cruz works well. I think this Ab is good for Western blot but not for the Elisa.
Date published: 2018-03-08
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Tau 抗体 (E-4) is rated 4.0 out of 5 by 2.
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