Date published: 2025-9-7
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Tau Antibody (Tau 46) - Western Blotting - Image 380253
Tau 抗体 (Tau 46): sc-32274. IMR-32 全细胞裂解液 (A) 和小鼠脑组织提取物 (B) 中 Tau 表达的 Western 印迹分析.
Tau 抗体 (Tau 46): sc-32274. IMR-32 全细胞裂解液 (A) 和小鼠脑组织提取物 (B) 中 Tau 表达的 Western 印迹分析.

Tau 抗体 (Tau 46): sc-32274

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说明书
  • Tau 抗体 Tau 46 是小鼠单克隆 IgG1 κ，Tau抗体, 在73篇文献中引用，规格为200 µg/ml
  • 培养自bovine来源的纯化的天然Tau，抗原表位为羧基酸的人Tau
  • Tau 抗体 (Tau 46) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 Tau and MAP-2 (280 kDa); 预计与所有六种Tau同种型以及牛Tau反应
  • 抗Tau抗体（Tau 46）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tau (Tau 46): sc-32274的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Tau Antibody (Tau 46) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Tau Antibody (Tau 46) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Tau抗体（Tau 46）是一种小鼠单克隆IgG1 kappa轻链抗体，用于识别纯化的天然牛源性Tau蛋白，其表位位于人类Tau的C端。抗Tau抗体（Tau 46）可检测小鼠、大鼠和人类中的Tau和MAP-2（280 kDa），可用于蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）等应用，预计可识别所有六种Tau同种型以及牛Tau。Tau，也称为MAPT（微管相关蛋白Tau）或TAU，是一种由758个氨基酸组成的蛋白质，在稳定神经细胞质、细胞骨架和细胞膜内的微管方面发挥着至关重要的作用。Tau的结构包括四个微管结合重复序列，它们对于促进微管组装和维持神经元极性至关重要，从而支持神经元的复杂结构和功能。Tau的结构灵活性使其能够适应各种细胞条件，促进轴突运输和突触功能等过程所需的细胞骨架可塑性。Tau以多种可变剪接形式存在，在神经元健康中发挥着不同的功能。Tau的翻译后修饰包括磷酸化、多泛素化和糖化，可调节其活性与代谢，确保细胞骨架动态平衡。Tau结构和功能的失调与多种神经退行性疾病有关，包括阿尔茨海默病、苍白球-桥脑-黑质变性（PPND）、皮质基底变性（CBD）和进行性核上性麻痹（PSP），这凸显了研究Tau在这些情况下的重要性。Tau 单克隆抗体（Tau 46）有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物，为研究人员提供了多种工具，用于研究 Tau 在健康和疾病中的结构和功能作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tau 抗体 (Tau 46) 参考文献:

  1. 额颞叶痴呆症和阿尔茨海默病患者脑脊液中的 tau 含量增加。  |  Green, AJ., et al. 1999. Neurosci Lett. 259: 133-5. PMID: 10025576
  2. 微管相关蛋白-tau是雌激素受体阳性乳腺癌内分泌敏感性和化疗耐药性的双功能预测因子。  |  Andre, F., et al. 2007. Clin Cancer Res. 13: 2061-7. PMID: 17404087
  3. tau 简史：神经退行性疾病中微管相关蛋白 tau 的演变。  |  Lace, GL., et al. 2007. Clin Neuropathol. 26: 43-58. PMID: 17416103
  4. Tau蛋白激酶：与阿尔茨海默病有关。  |  Martin, L., et al. 2013. Ageing Res Rev. 12: 289-309. PMID: 22742992
  5. Tau蛋白病和Tau蛋白低聚物。  |  Takashima, A. 2013. J Alzheimers Dis. 37: 565-8. PMID: 23948895
  6. 细胞外Tau及其信号的来源。  |  Avila, J., et al. 2014. J Alzheimers Dis. 40 Suppl 1: S7-S15. PMID: 24531154
  7. 神经生物学和病理学中Tau蛋白的液-液相分离。  |  Wegmann, S. 2019. Adv Exp Med Biol. 1184: 341-357. PMID: 32096048
  8. 微管相关蛋白 tau 表位存在于各种肌肉疾病的纤维病变中。  |  Lübke, U., et al. 1994. Am J Pathol. 145: 175-88. PMID: 7518193
  9. 在人类和啮齿类动物的培养细胞系中，一种类似 tau 的蛋白质与应力纤维和微管相互作用。  |  Cross, D., et al. 1993. J Cell Sci. 105 (Pt 1): 51-60. PMID: 7689576
  10. 脑内 tau 蛋白激酶 I/ 糖原合成酶激酶 3beta 对线粒体丙酮酸脱氢酶活性的调控  |  Hoshi, M., et al. 1996. Proc Natl Acad Sci U S A. 93: 2719-23. PMID: 8610107
  11. 几种蛋白激酶对含有三个重复序列的人类 tau 异构体的不同磷酸化作用。  |  Singh, TJ., et al. 1996. Arch Biochem Biophys. 328: 43-50. PMID: 8638936
  12. 蛋白磷酸酶抑制剂会导致培养的大鼠海马神经元的突触结构改变和 tau 过度磷酸化。  |  Malchiodi-Albedi, F., et al. 1997. J Neurosci Res. 48: 425-38. PMID: 9185666

订购信息

产品名称产品编号规格价格数量收藏夹

Tau 抗体 (Tau 46)

sc-32274
200 µg/ml
RMB2377.00

Tau (Tau 46): m-IgGκ BP-HRP 套装

sc-520832
200 µg Ab, 40 µg BP
RMB2662.00

Tau (Tau 46): m-IgG1 BP-HRP 套装

sc-533471
200 µg Ab; 20 µg BP
RMB2662.00

Tau 抗体 (Tau 46) AC

sc-32274 AC
500 µg/ml, 25% agarose
RMB3129.00

Tau 抗体 (Tau 46) HRP

sc-32274 HRP
200 µg/ml
RMB2377.00

Tau 抗体 (Tau 46) FITC

sc-32274 FITC
200 µg/ml
RMB2482.00

Tau 抗体 (Tau 46) PE

sc-32274 PE
200 µg/ml
RMB2580.00

Tau 抗体 (Tau 46) Alexa Fluor® 488

sc-32274 AF488
200 µg/ml
RMB2685.00

Tau 抗体 (Tau 46) Alexa Fluor® 546

sc-32274 AF546
200 µg/ml
RMB2685.00

Tau 抗体 (Tau 46) Alexa Fluor® 594

sc-32274 AF594
200 µg/ml
RMB2685.00

Tau 抗体 (Tau 46) Alexa Fluor® 647

sc-32274 AF647
200 µg/ml
RMB2685.00

Tau 抗体 (Tau 46) Alexa Fluor® 680

sc-32274 AF680
200 µg/ml
RMB2685.00

Tau 抗体 (Tau 46) Alexa Fluor® 790

sc-32274 AF790
200 µg/ml
RMB2685.00

Hi how can I order Anti-Tau Antibody (Tau 46) conjugated to agarose for IP?

Asked by: Emily
Thank you for your question. sc-32274 AC is recommended for IP. Here is our protocol : https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: BlakeJ
Date published: 2025-09-01

这个抗体原液以及用奶粉配好的稀释后的抗体可以放-20吗？会影响抗体作用吗

Asked by: tau46
感谢您的提问。Tau 抗体 (Tau 46): sc-32274抗体原液及稀释液不可以放在-20°C冷冻，冷冻后的抗体无法提供产品质保。 Thank you for your question. Tau antibody (Tau 46): sc-32274 and its dilution can't be stored at -20°C, there is no product warranty once it is frozen.
Answered by: Technical Service SH
Date published: 2025-08-11

Does this antibody also recognize/cross-react MAP2? Can you provide the epitope sequence or information? Thank you very much!

Asked by: CSYang
Thank you for your question. Yes, this antibody also cross reacts with MAP-2 (280 kDa). The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-03-27

Is there a possibility to order this from rabbit host? Thanks

Asked by: Keagan
Thank you for your question. Tau Antibody (Tau 46): sc-32274 and all other antibodies we offer that are specific against Tau are only produced in mouse. Please contact our technical service for assistance. We can offer a variety of options that may suit your particular need in this case.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the recommended secondary reagent to use with sc-32274: Tau (Tau 46) mouse monoclonal primary?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with sc-32274: Tau (Tau 46), we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-10
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Rated 5 out of 5 by from Good antibodyVery good antibody for ICC and IHC, detects neurons of mouse brain sections or primary mouse neuron cultures with a dilution of 1:500.
Date published: 2023-02-01
Rated 4 out of 5 by from bande a basso PML'anticorpo TAU 46 è stato saggiato su estratto cellulare totale derivante dalla linea cellulare SH-SY5Y. Brevemente, le cellule sono state seminate alla densità di 4x105 cells/plate su piastra da 6 pozzetti (35mm) in DMEM high glucose fino alla confluenza del 70-80% in atmosfera umidificata al 5% CO2 a 37°C. Successivamente sono state recuperate, lisate con lysis buffer in presenza di inibitori delle proteasi e dosate spettrofotometricamente. 30 ug di estratto proteico, dopo precipitazione in acetone glaciale per 1h a -20°C, è stato ripreso con 15 ul di Laemmli buffer in DTE e caricati su 10% Tris-glicina SDS-PAGE. Dopo corsa elettroforetica, si è proceduto con trasferimento su PVDF attivata. La membrana, dopo lavaggi in TBS 1X, è stata bloccata con BSA 5% in TBS 1X t.a. per 1h e successivamente incubata con sample SC32274 over night in agitazione a 4°C dil 1:1000 in TBS 1X+BSA 3%.Dopo 3 lavaggi con TBS 1X, la membrana è stata incubata con anticorpo secondario dil 1:10000 t.a per 1h. Quindi dopo 3 lavaggi si è proceduto con sviluppo ECL e acquisizione al Chemidoc. In base al risultato ottenuto vorrei chiedere se le bande individuate (PM circa 55-60 kDA la più abbondante e più alta e circa 20-30 kDA la più bassa) sono compatibili con TAU totale. Se affermativo come si spiega la forma a più basso peso molecolare? può essere una forma clivata? avete dati in merito? Sarebbe auspicabile, qualora fosse disponibile, ricevere un controllo positivo.
Date published: 2021-09-09
Rated 4 out of 5 by from 1: 250 dilution works for Western blot,I use this one to do Western blot using 20-30ug mouse brain lysates. It worked well, clean and clear band in my case. Recommended.
Date published: 2020-02-05
Rated 5 out of 5 by from Marker for AxonsThis antibody was used to look at Axons in differentiated neurons
Date published: 2019-10-05
Rated 4 out of 5 by from Good antibodyAntibody works quite well on tissue sections (IF and IHC).
Date published: 2018-06-07
Rated 4 out of 5 by from Good for IFThis Tau Antibody (Tau 46), sc-32274, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 4 out of 5 by from Tau我使用的是该抗体的小样试用装，以1:200稀释用作Western Blot实验，首次孵育时条带较清晰，第二第三次再使用时效果没那么好
Date published: 2017-11-23
Rated 5 out of 5 by from Good Ab for WBWorked in Western Blot using mouse brain. Nice specific band.
Date published: 2017-10-30
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Tau 抗体 (Tau 46) is rated 4.5 out of 5 by 24.
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