Date published: 2025-9-7

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TAP 抗体 (53H8): sc-32319

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说明书
  • TAP 抗体 53H8 是小鼠单克隆 IgG1 κ,TAP抗体, 在11篇文献中引用,规格为200 µg/ml
  • 针对human TAP
  • TAP 抗体 (53H8) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 TAP
  • 抗TAP抗体(53H8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TAP (53H8): sc-32319免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 TAP 抗体 (53H8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TAP 抗体 (53H8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

TAP抗体(53H8)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠和人类来源的TAP蛋白。抗TAP抗体(53H8)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。TAP蛋白(也称为NXF1)在mRNA从细胞核向细胞质出口的过程中起着至关重要的作用,这对于基因的正确表达和细胞功能至关重要。TAP与D型逆转录病毒的组成性转运元件(CTE)相互作用,促进未拼接的病毒RNA的核出口。这种相互作用能够促进病毒的高效复制和新病毒颗粒的产生。TAP属于NXF蛋白家族,该家族具有保守的富含亮氨酸重复结构域和核转运因子2样结构域,突出了该蛋白家族在细胞转运机制中的进化意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TAP 抗体 (53H8) 参考文献:

  1. 人类Tap蛋白是一种核mRNA出口因子,含有新型的RNA结合和核质运输序列。  |  Kang, Y. and Cullen, BR. 1999. Genes Dev. 13: 1126-39. PMID: 10323864
  2. 分析人类tap蛋白的RNA结合特异性,该蛋白是一种构成性转运元件特异性核RNA出口因子。  |  Kang, Y., et al. 1999. Virology. 262: 200-9. PMID: 10489353
  3. TAP蛋白对Ped基因表达的调控。  |  Ke, X. and Warner, CM. 2000. J Reprod Immunol. 46: 1-15. PMID: 10708239
  4. TAP(NXF1)属于假定 RNA 导出因子的多基因家族,具有保守的模块化结构。  |  Herold, A., et al. 2000. Mol Cell Biol. 20: 8996-9008. PMID: 11073998
  5. 秀丽隐杆线虫的 mRNA 导出是由 Ce-NXF-1 介导的,Ce-NXF-1 是人类 TAP/NXF 和酿酒酵母 Mex67p 的同源物。  |  Tan, W., et al. 2000. RNA. 6: 1762-72. PMID: 11142376
  6. TAP/p15 异源二聚体的过表达可绕过核保留并刺激核 mRNA 的输出。  |  Braun, IC., et al. 2001. J Biol Chem. 276: 20536-43. PMID: 11259411
  7. 利用rev和RRE突变的人类免疫缺陷病毒1型DNA前病毒克隆作为分子陷阱,鉴定新型转录后调控元件。  |  Nappi, F., et al. 2001. J Virol. 75: 4558-69. PMID: 11312326
  8. 逆转录病毒组成型转运元件是从细胞 TAP(NXF1)结合序列演化而来的。  |  Zolotukhin, AS., et al. 2001. J Virol. 75: 5567-75. PMID: 11356964
  9. 在小鼠静脉注射1型单纯疱疹病毒后,ICP47通过诱导TAP蛋白介导病毒神经侵袭。  |  Burgos, JS., et al. 2006. J Neurovirol. 12: 420-7. PMID: 17162658
  10. 细胞RNA出口受体TAP/NXF1是ICP27介导的1型单纯疱疹病毒RNA出口所必需的,但TREX复合物适配蛋白Aly/REF似乎可有可无。  |  Johnson, LA., et al. 2009. J Virol. 83: 6335-46. PMID: 19369354
  11. 在天然细胞膜环境中分析转运蛋白与抗原处理(TAP)蛋白的构象状态。  |  Geng, J., et al. 2013. J Biol Chem. 288: 37039-47. PMID: 24196954
  12. TAP蛋白及相关Ly-6连接分子的结构和功能。  |  Reiser, H., et al. 1988. Year Immunol. 3: 80-8. PMID: 2831682

订购信息

产品名称产品编号规格价格数量收藏夹

TAP 抗体 (53H8)

sc-32319
200 µg/ml
RMB2377.00

TAP (53H8): m-IgG Fc BP-HRP 套装

sc-528463
200 µg Ab; 10 µg BP
RMB2662.00

TAP (53H8): m-IgGκ BP-HRP 套装

sc-520846
200 µg Ab, 40 µg BP
RMB2662.00

TAP (53H8): m-IgG1 BP-HRP 套装

sc-542943
200 µg Ab; 20 µg BP
RMB2662.00

TAP 抗体 (53H8) AC

sc-32319 AC
500 µg/ml, 25% agarose
RMB3129.00

TAP 抗体 (53H8) HRP

sc-32319 HRP
200 µg/ml
RMB2377.00

TAP 抗体 (53H8) FITC

sc-32319 FITC
200 µg/ml
RMB2482.00

TAP 抗体 (53H8) PE

sc-32319 PE
200 µg/ml
RMB2580.00

TAP 抗体 (53H8) Alexa Fluor® 488

sc-32319 AF488
200 µg/ml
RMB2685.00

TAP 抗体 (53H8) Alexa Fluor® 546

sc-32319 AF546
200 µg/ml
RMB2685.00

TAP 抗体 (53H8) Alexa Fluor® 594

sc-32319 AF594
200 µg/ml
RMB2685.00

TAP 抗体 (53H8) Alexa Fluor® 647

sc-32319 AF647
200 µg/ml
RMB2685.00

TAP 抗体 (53H8) Alexa Fluor® 680

sc-32319 AF680
200 µg/ml
RMB2685.00

TAP 抗体 (53H8) Alexa Fluor® 790

sc-32319 AF790
200 µg/ml
RMB2685.00

Will this antibody detect a protein that contain the first 240 amino acids of Nxf1 ? Can you reveal the epitope, otherwise I cannot use this antibody.

Asked by: LouH
Thank you for your question. The exact immunogen used to develop this monoclonal antibody, TAP (53H8): sc-32319, is not known, and the epitope has not been mapped. We have two alternative antibodies available that do have epitopes that are mapping within the first 240 amino acids of NFX1, or the TAP protein. Links to the information for these antibodies are provided below as a reference. TAP (G-12): sc-28377 https://www.scbt.com/scbt/product/tap-antibody-g-12 TAP (D-9): sc-365576 https://www.scbt.com/scbt/product/tap-antibody-d-9
Answered by: Technical Support
Date published: 2017-07-24

I am using TAP (53H8): sc-32319 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodyGood results when used in western blot of human cells.
Date published: 2021-05-31
Rated 2 out of 5 by from works OKsignal not so strong as compared to the one that we already are using.. makes use of too much antibody
Date published: 2017-11-07
Rated 4 out of 5 by from Good for WBit works well for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 4 out of 5 by from Good quality for IF analysisThis antibody gave a positive staining when use it detect TAP in mouse lung tissue, I obtained specific staining.
Date published: 2016-12-21
Rated 5 out of 5 by from Produced superb immunoperoxidase nuclearProduced superb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human fallopian tube tissue. -SCBT QC
Date published: 2015-05-21
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TAP 抗体 (53H8) is rated 4.0 out of 5 by 6.
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