Date published: 2025-9-6

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T2R3 抗体 (E-12): sc-398489

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说明书
  • T2R3抗体(E-12)是小鼠单克隆IgG1 λ T2R3抗体, 在1篇文献中引用,规格为200 µg/ml
  • 抗原表位位于human来源T2R3胞外域的72-89氨基酸之间
  • T2R3 抗体 (E-12) 推荐用于 WB, IP, IF 和 ELISA,检测 T2R3 of human origin, T2R37 of mouse origin and the corresponding rat homolog
  • 抗T2R3抗体(E-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取T2R3 (E-12): sc-398489免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP(小鼠 IgGλ 结合蛋白-HRP)是T2R3 Antibody (E-12) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与T2R3 Antibody (E-12) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGλ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

T2R3抗体(E-12)是一种IgG1 λ小鼠单克隆T2R3抗体(也称为T2R3抗体),可通过WB、IP、IF和ELISA检测到来源于小鼠、大鼠和人类的T2R3蛋白。T2R3抗体(E-12)既有无偶联的抗T2R3抗体形式,也有多种偶联形式的抗T2R3抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。无内含子的味觉受体基因编码一类七次跨膜受体蛋白家族,这些蛋白作为苦味受体发挥作用。其中一个成员是T2R3(味觉受体类型2成员3),也称为TAS2R3,它是一种316个氨基酸的多次跨膜蛋白,属于G蛋白偶联受体T2R家族。T2R3在口腔和胃肠道中发挥作用,是一种与味导素偶联的受体,参与苦味化合物的感知。T2R3通过PLC β2(一种选择性表达于味觉组织中的磷脂酶C)和钙调节阳离子通道TRPM5介导对某些味觉的反应。T2R3在舌头和腭上皮的味觉受体细胞亚群以及味导素阳性细胞中表达,同时在胃的胃窦和胃底(胃的一部分)、十二指肠和胃内分泌细胞中表达。编码T2R3的基因包含约1101个碱基,定位于人类7号染色体q34区域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

T2R3 抗体 (E-12) 参考文献:

  1. 哺乳动物味觉受体新家族  |  Adler, E., et al. 2000. Cell. 100: 693-702. PMID: 10761934
  2. T2R 具有苦味受体的功能。  |  Chandrashekar, J., et al. 2000. Cell. 100: 703-11. PMID: 10761935
  3. 大量的味觉感受器  |  Kinnamon, SC. 2000. Neuron. 25: 507-10. PMID: 10774719
  4. 人类味觉受体基因中涉及苦味的编码单核苷酸多态性的鉴定。  |  Ueda, T., et al. 2001. Biochem Biophys Res Commun. 285: 147-51. PMID: 11437385
  5. 苦味和甜味转导的分子机制  |  Margolskee, RF. 2002. J Biol Chem. 277: 1-4. PMID: 11696554
  6. 苦味和甜味受体  |  Montmayeur, JP. and Matsunami, H. 2002. Curr Opin Neurobiol. 12: 366-71. PMID: 12139982
  7. 甜味, 苦味和鲜味的编码:不同的受体细胞共享相似的信号通路。  |  Zhang, Y., et al. 2003. Cell. 112: 293-301. PMID: 12581520
  8. 人类和猿类苦味受体的进化  |  Fischer, A., et al. 2005. Mol Biol Evol. 22: 432-6. PMID: 15496549

订购信息

产品名称产品编号规格价格数量收藏夹

T2R3 抗体 (E-12)

sc-398489
200 µg/ml
RMB2377.00

T2R3 (E-12): m-IgGλ BP-HRP 套装

sc-524364
200 µg Ab, 40 µg BP
RMB2662.00

T2R3 抗体 (E-12) AC

sc-398489 AC
500 µg/ml, 25% agarose
RMB3129.00

T2R3 抗体 (E-12) HRP

sc-398489 HRP
200 µg/ml
RMB2377.00

T2R3 抗体 (E-12) FITC

sc-398489 FITC
200 µg/ml
RMB2482.00

T2R3 抗体 (E-12) PE

sc-398489 PE
200 µg/ml
RMB2580.00

T2R3 抗体 (E-12) Alexa Fluor® 488

sc-398489 AF488
200 µg/ml
RMB2685.00

T2R3 抗体 (E-12) Alexa Fluor® 546

sc-398489 AF546
200 µg/ml
RMB2685.00

T2R3 抗体 (E-12) Alexa Fluor® 594

sc-398489 AF594
200 µg/ml
RMB2685.00

T2R3 抗体 (E-12) Alexa Fluor® 647

sc-398489 AF647
200 µg/ml
RMB2685.00

T2R3 抗体 (E-12) Alexa Fluor® 680

sc-398489 AF680
200 µg/ml
RMB2685.00

T2R3 抗体 (E-12) Alexa Fluor® 790

sc-398489 AF790
200 µg/ml
RMB2685.00

T2R3 (E-12) 中和勝肽

sc-398489 P
100 µg/0.5 ml
RMB511.00

Which secondary antibody can I use if i am using the Sc-398489 FITC antibody for T2R3 as my primary? Also, is FITCA for ELISA ?

Asked by: Brayan
Thank you for your question. You do not need to use any secondary antibody for detection if you are using the directly conjugated primary antibody, sc-398489 FITC. This catalog number refers to this primary antibody directly conjugated to the fluorescent dye, fluoroscein (FITC). We do not have any protocol for using a FITC onjugated antibody for ELISA. Please contact us for further technical support.
Answered by: Technical Support
Date published: 2019-04-13

What application is the blocking peptide sc-398489 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2018-06-07
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Rated 5 out of 5 by from Positive results in WB using human stomachPositive results in WB using human stomach tissue extract. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by from Excellent immunofluorescence membrane stainingExcellent immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Great antibody for western blot; strong bandsGreat antibody for western blot; strong bands in HeLa, A549, Jurkat, A-431 and SCC-25 whole cell lysates. -SCBT QC
Date published: 2015-01-30
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T2R3 抗体 (E-12) is rated 5.0 out of 5 by 3.
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