Date published: 2025-9-7
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Sulf-2 Antibody (G-4) - Western Blotting - Image 360077
Sulf-2 抗体 (G-4): sc-271772. Sulf-2在 MCF7 (A), Y79 (B), c4 (C), NIH/3T3 (D), RPE-J (E) 和 RAT2 (F) 全细胞裂解液中表达的 Western 印迹分析.
Sulf-2 抗体 (G-4): sc-271772. Sulf-2在 MCF7 (A), Y79 (B), c4 (C), NIH/3T3 (D), RPE-J (E) 和 RAT2 (F) 全细胞裂解液中表达的 Western 印迹分析.

Sulf-2 抗体 (G-4): sc-271772

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说明书
  • Sulf-2 抗体 G-4 是小鼠单克隆 IgG1 κ，Sulf-2抗体, 在4篇文献中引用，规格为200 µg/ml
  • 与human的Sulf-2的细胞外结构域内的氨基酸481-560杂交
  • Sulf-2 抗体 (G-4) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Sulf-2
  • 抗Sulf-2抗体（G-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Sulf-2 (G-4): sc-271772的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Sulf-2 Antibody (G-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Sulf-2 Antibody (G-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Sulf抗体

Sulf-2抗体（G-4）是一种IgG1 κ小鼠单克隆Sulf-2抗体（也被指定为Sulf-2抗体），能够通过WB、IP、IF和ELISA检测到小鼠、大鼠和人类来源的Sulf-2蛋白。Sulf-2抗体（G-4）既有非偶联型抗Sulf-2抗体形式，也有多种偶联型抗Sulf-2抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Sulf-2（硫酸酯酶2），也被称为HSULF-2，是一种属于硫酸酯酶家族的细胞外内硫酸酯酶。硫酸酯酶家族的成员各自含有一个保守的活性位点，该活性位点具有翻译后生成的α-甲酰甘氨酸，这对它们的催化活性至关重要。这些酶负责水解硫酸酯键。Sulf-1（硫酸酯酶1）和Sulf-2特异性地与肝素硫酸盐蛋白聚糖（HSPGs）相互作用，并水解葡糖胺-6-硫酸盐修饰，从而调节HSPGs与各种信号分子的相互作用。作为细胞表面和细胞外基质的关键组成部分，HSPGs调节生长因子活性，从而影响细胞生长和分化。此外，HSPGs通过介导细胞粘附和生长因子及血管生成因子的活性，在调节肿瘤细胞转移中发挥关键作用。这表明Sulf-1和Sulf-2在肿瘤进展中发挥重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Sulf-2 抗体 (G-4) 参考文献:

  1. 小鼠和人类两种细胞外肝素降解内硫酸酯酶的克隆和特征。  |  Morimoto-Tomita, M., et al. 2002. J Biol Chem. 277: 49175-85. PMID: 12368295
  2. 利用质谱分析肝素/硫酸肝素的成分剖析：新型细胞外人类内硫酸酯酶特异性的检测。  |  Saad, OM., et al. 2005. Glycobiology. 15: 818-26. PMID: 15843596
  3. HSulf-1 和 HSulf-2 是骨髓瘤肿瘤体内生长的有效抑制剂。  |  Dai, Y., et al. 2005. J Biol Chem. 280: 40066-73. PMID: 16192265
  4. Sulf-2是一种促血管生成的硫酸肝素内硫酸化酶，在乳腺癌中上调。  |  Morimoto-Tomita, M., et al. 2005. Neoplasia. 7: 1001-10. PMID: 16331886
  5. 细胞外葡萄糖胺-6-硫酸化酶 HSulf-2 可选择性地调动肝素结合的生长因子和趋化因子：对血管内皮生长因子, 成纤维细胞生长因子-1 和成纤维细胞生长因子-1 的影响。  |  Uchimura, K., et al. 2006. BMC Biochem. 7: 2. PMID: 16417632
  6. 人类乳腺癌和结直肠癌的共识编码序列。  |  Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
  7. 测量 Sulfs 的活性：两种新型肝素/硫酸肝素内硫酸酯酶。  |  Uchimura, K., et al. 2006. Methods Enzymol. 416: 243-53. PMID: 17113870
  8. 胞外硫酸酯酶是 Wnt 信号通路的组成部分，对人类胰腺癌细胞的生长和致瘤性有积极的调节作用。  |  Nawroth, R., et al. 2007. PLoS One. 2: e392. PMID: 17460759
  9. 人类硫酸酯酶：从结构角度看催化作用。  |  Ghosh, D. 2007. Cell Mol Life Sci. 64: 2013-22. PMID: 17558559
  10. Sulf-2：细胞信号传导的细胞外调节剂和癌症候选靶标。  |  Rosen, SD. and Lemjabbar-Alaoui, H. 2010. Expert Opin Ther Targets. 14: 935-49. PMID: 20629619
  11. 胞外硫磺酯酶Sulf-2含有软骨素/皮肤素硫酸链，可调节其酶活性。  |  El Masri, R., et al. 2022. Cell Rep. 38: 110516. PMID: 35294879
  12. Galectin-3结合蛋白抑制胞外肝素6-O-硫酯酶Sulf-2。  |  Panigrahi, A., et al. 2024. Mol Cell Proteomics. 23: 100793. PMID: 38825040

订购信息

产品名称产品编号规格价格数量收藏夹

Sulf-2 抗体 (G-4)

sc-271772
200 µg/ml
RMB2377.00

Sulf-2 (G-4): m-IgG Fc BP-HRP 套装

sc-529259
200 µg Ab; 10 µg BP
RMB2662.00

Sulf-2 (G-4): m-IgGκ BP-HRP 套装

sc-522057
200 µg Ab, 40 µg BP
RMB2662.00

Sulf-2 (G-4): m-IgG1 BP-HRP 套装

sc-543419
200 µg Ab; 20 µg BP
RMB2662.00

Sulf-2 抗体 (G-4) AC

sc-271772 AC
500 µg/ml, 25% agarose
RMB3129.00

Sulf-2 抗体 (G-4) HRP

sc-271772 HRP
200 µg/ml
RMB2377.00

Sulf-2 抗体 (G-4) FITC

sc-271772 FITC
200 µg/ml
RMB2482.00

Sulf-2 抗体 (G-4) PE

sc-271772 PE
200 µg/ml
RMB2580.00

Sulf-2 抗体 (G-4) Alexa Fluor® 488

sc-271772 AF488
200 µg/ml
RMB2685.00

Sulf-2 抗体 (G-4) Alexa Fluor® 546

sc-271772 AF546
200 µg/ml
RMB2685.00

Sulf-2 抗体 (G-4) Alexa Fluor® 594

sc-271772 AF594
200 µg/ml
RMB2685.00

Sulf-2 抗体 (G-4) Alexa Fluor® 647

sc-271772 AF647
200 µg/ml
RMB2685.00

Sulf-2 抗体 (G-4) Alexa Fluor® 680

sc-271772 AF680
200 µg/ml
RMB2685.00

Sulf-2 抗体 (G-4) Alexa Fluor® 790

sc-271772 AF790
200 µg/ml
RMB2685.00

Perchè l'anticorpo anti sulf2 (G-4): sc-271772 in WB riconosce (come da immagini di esempio mostrate nel datasheet) proteine con MW differente a seconda delle linee cellulari analizzate? Grazie.

Asked by: Pasquale78
La ringraziamo per la sua domanda, la proteina umana Sulf-2 consiste in 870 amminoacidi con un peso molecolare predetto di circa 100 kDa ed un peso molecolare osservato di circa 68 kDa. La migrazione proteica nel gel può essere influenzata da diverse modificazioni post-traduzionali, così l'altezza delle bande osservate può differire da quelle predette. Doppietti o bande multiple possono evidenziarsi a seconda del materiale campione e delle condizioni di corsa utilizzate per il gel. L'immagine nel datasheet mostra segnali specifici per il materiale trasfettato, ma non è rappresentativo per la proteina endogena. Per favore contattare il servizio tecnico per ulteriori informazioni. Thank you very much for your inquiry. The human Sulf-2 full-length protein consists of 870 amino acids with a predicted molecular weight around 100kDa and an observed molecular weight around 68 kDa in a Western Blot. The protein migration in the gel will be affected by different post-translational modifications, so that the actual band sizes observed may differ from the predicted size. Also a doublet or multiple bands might be observed occasionally depending on the Sample material and conditions used to run the actual gel. The Lane B in the Datasheet show specific signal for transfected date, but won't be representative of the endogenous protein. Please contact our technical support for further information.
Answered by: Technical Support Europe
Date published: 2017-03-13

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-271772: Sulf-2 (G-4) mouse monoclonal antibody will work?

Asked by: TinTin
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Great antibodyThis antibody works extremely well for WB on mouse cells.
Date published: 2019-08-19
Rated 5 out of 5 by from Great Western Blot data of SulfGreat Western Blot data of Sulf-2 expression in non-transfected and human Sulf-2 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Produced positive Western Blot data of SulfProduced positive Western Blot data of Sulf-2 expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2014-12-18
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Sulf-2 抗体 (G-4) is rated 5.0 out of 5 by 3.
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