Date published: 2025-9-6

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Stat5b 抗体 (G-2): sc-1656

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说明书
  • Stat5b抗体(G-2)是小鼠单克隆IgG1 κ, 在141篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的Stat5b的C-terminus的氨基酸750-779之间
  • 抗-Stat5b 抗体 (G-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Stat5b; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗Stat5b抗体(G-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-1656 X, 200 µg/0.1 ml)
  • 关于如何获取Stat5b (G-2): sc-1656免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Stat5b 抗体 (G-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Stat5b 抗体 (G-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Stat5b抗体(G-2)是一种小鼠单克隆IgG1 kappa轻链抗体,可特异性识别小鼠源Stat5b C末端750-779氨基酸之间的表位。Stat5b单克隆抗体(G-2)可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHC)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的Stat5b,还可与马、犬、牛和猪等其他物种发生反应。Stat5b,即信号转导和转录激活因子5b,也称为STAT5和GHISID2,是一种重要的转录因子,在细胞因子和生长因子信号传导通路中发挥着关键作用。Stat5b在受到IL-2、IL-4、CSF-1和生长激素等刺激时会被激活,通常是通过受体酪氨酸激酶或G蛋白偶联受体激活的。Stat5b经历磷酸化、二聚化和核转移,以调节基因表达,影响T细胞受体信号传导、细胞凋亡、成人乳腺发育和肝脏基因表达的性二态性。Stat5b的重要性在于它是一种主要在肝脏表达的Stat5形式,通过与视黄酸受体α基因融合而与急性早幼粒细胞白血病(APLL)有关。对Stat5b缺失小鼠的研究表明,淋巴发育和分化严重受损,这凸显了它在造血和免疫功能中的重要性。Stat5b 单克隆抗体(G-2)有非结合型和多种结合型,包括琼脂糖、HRP、PE、FITC 和各种 Alexa Fluor® 结合物,为各种研究应用提供了多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Stat5b 抗体 (G-2) 参考文献:

  1. Stat5a和Stat5b在IL-2家族细胞因子信号传导中的作用  |  Lin, JX. and Leonard, WJ. 2000. Oncogene. 19: 2566-76. PMID: 10851055
  2. Stat5a/b有助于白细胞介素7诱导的B细胞前体扩增,但abl-和bcr/abl-诱导的转化与stat5无关。  |  Sexl, V., et al. 2000. Blood. 96: 2277-83. PMID: 10979977
  3. 转录因子 STAT5A 是 B 细胞中布鲁顿酪氨酸激酶的底物。  |  Mahajan, S., et al. 2001. J Biol Chem. 276: 31216-28. PMID: 11413148
  4. GH激活的转录信号转导子和激活子5a(STAT5a)和STAT5b的丝氨酸磷酸化:对STAT5转录活性的影响。  |  Park, SH., et al. 2001. Mol Endocrinol. 15: 2157-71. PMID: 11731617
  5. STAT5a 激活介导致癌物质 RhoA 诱导的上皮细胞向间质转化。  |  Benitah, SA., et al. 2003. Mol Biol Cell. 14: 40-53. PMID: 12529425
  6. 特异性抑制Stat5a/b可促进IL-2反应性原代淋巴细胞和肿瘤衍生淋巴细胞的凋亡。  |  Behbod, F., et al. 2003. J Immunol. 171: 3919-27. PMID: 14530308
  7. STAT5A/5B 缺陷小鼠的耐受性丧失和自身免疫性影响多个器官  |  Snow, JW., et al. 2003. J Immunol. 171: 5042-50. PMID: 14607901
  8. STAT5A 与μ-阿片受体相互作用,并在激活后磷酸化。  |  Mazarakou, G. and Georgoussi, Z. 2005. J Neurochem. 93: 918-31. PMID: 15857395
  9. ERBB4/HER4 通过调节新型 STAT5A 丝氨酸磷酸化事件来增强 STAT5A 的转录活性。  |  Clark, DE., et al. 2005. J Biol Chem. 280: 24175-80. PMID: 15863494
  10. Stat5a/b 对正常淋巴细胞的发育和分化至关重要。  |  Yao, Z., et al. 2006. Proc Natl Acad Sci U S A. 103: 1000-5. PMID: 16418296
  11. 中心体相关CDC25B是一种新的致病基因,可导致白内障、扩张型心肌病和多种内分泌疾病。  |  Hwa, V. 2016. Growth Horm IGF Res. 28: 16-20. PMID: 26703237
  12. STAT5b 是 NRG-1/ERBB4 介导的心肌生长的关键效应器。  |  Vaparanta, K., et al. 2023. EMBO Rep. 24: e56689. PMID: 37009825

订购信息

产品名称产品编号规格价格数量收藏夹

Stat5b 抗体 (G-2)

sc-1656
200 µg/ml
RMB2377.00

Stat5b (G-2): m-IgG Fc BP-HRP 套装

sc-528130
200 µg Ab; 10 µg BP
RMB2662.00

Stat5b (G-2): m-IgGκ BP-HRP 套装

sc-520433
200 µg Ab, 40 µg BP
RMB2662.00

Stat5b (G-2): m-IgG1 BP-HRP 套装

sc-542779
200 µg Ab; 20 µg BP
RMB2662.00

Stat5b 抗体 (G-2) X

sc-1656 X
200 µg/0.1 ml
RMB2377.00

Stat5b 抗体 (G-2) AC

sc-1656 AC
500 µg/ml, 25% agarose
RMB3129.00

Stat5b 抗体 (G-2) HRP

sc-1656 HRP
200 µg/ml
RMB2377.00

Stat5b 抗体 (G-2) FITC

sc-1656 FITC
200 µg/ml
RMB2482.00

Stat5b 抗体 (G-2) PE

sc-1656 PE
200 µg/ml
RMB2580.00

Stat5b 抗体 (G-2) Alexa Fluor® 488

sc-1656 AF488
200 µg/ml
RMB2685.00

Stat5b 抗体 (G-2) Alexa Fluor® 546

sc-1656 AF546
200 µg/ml
RMB2685.00

Stat5b 抗体 (G-2) Alexa Fluor® 594

sc-1656 AF594
200 µg/ml
RMB2685.00

Stat5b 抗体 (G-2) Alexa Fluor® 647

sc-1656 AF647
200 µg/ml
RMB2685.00

Stat5b 抗体 (G-2) Alexa Fluor® 680

sc-1656 AF680
200 µg/ml
RMB2685.00

Stat5b 抗体 (G-2) Alexa Fluor® 790

sc-1656 AF790
200 µg/ml
RMB2685.00

Stat5b (G-2) 中和勝肽

sc-1656 P
100 µg/0.5 ml
RMB511.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Germaine
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-09

What is the difference between sc-1656 and sc-1656 P?

Asked by: AbPolly
Thank you for your inquiry. sc-1656 is our standard unconjugated Stat5b (G-2) monoclonal antibody. sc-1656 P is the blocking peptide matching this antibody that can be used as a negative control in combination with the antibody. For the protocol on how to use the blocking peptide, please see here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Service
Date published: 2016-12-23
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Rated 4 out of 5 by from Stat5b抗体之前申购过一次uPA的抗体,效果还不错,这次又申购了一批抗体,跑出来的效果还可以,考虑买1支继续使用
Date published: 2017-10-23
Rated 2 out of 5 by from Unspecific bands with longer exposure timeThe antibody shows unespecific bands if used longer exposure times (such as 1 min). Using shorter exposure times you will have your band at the correct size.
Date published: 2017-05-14
Rated 5 out of 5 by from Strong WB bandTarget bands easily observed in Human cancer cells
Date published: 2016-12-23
Rated 5 out of 5 by from Absolutely publishable data obtainedmy bands were very nice and crisp, way better than the ones shown in the figure above. absolutely recommended! used on whole cell lysate for WB
Date published: 2016-11-10
Rated 5 out of 5 by from Nice quality IF image obtained using HepG2 cellsNice quality IF image obtained using HepG2 cells. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Good staining patternGood staining pattern, IHC(P) human adrenal gland. -SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by from Great antibody for WesternGreat antibody for Western, clean band detected in Jurkat and BJAB cells. -SCBT QC
Date published: 2014-09-03
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Stat5b 抗体 (G-2) is rated 4.4 out of 5 by 7.
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