Date published: 2025-9-6
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Stat5 Antibody (A-9) - Western Blotting - Image 350004
Stat5 抗体 (A-9): sc-74442. HeLa (A), BJAB (B) 和 CCRF-CEM (C) 全细胞裂解液中 Stat5 表达的 Western 印迹分析.
Stat5 抗体 (A-9): sc-74442. HeLa (A), BJAB (B) 和 CCRF-CEM (C) 全细胞裂解液中 Stat5 表达的 Western 印迹分析.

Stat5 抗体 (A-9): sc-74442

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寫評論提問

说明书
  • Stat5 抗体 A-9 是小鼠单克隆 IgG1 κ，Stat5抗体, 在39篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Stat5a的氨基酸661-794
  • Stat5 抗体 (A-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Stat5a and Stat5b
  • 抗Stat5抗体（A-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74442 X, 200 µg/0.1 ml)
  • 关于如何获取Stat5 (A-9): sc-74442的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Stat5 抗体 (A-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Stat5 抗体 (A-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Stat抗体，包括Stat1, Stat2, Stat3, Stat4, Stat5和Stat6

Stat5抗体（A-9）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测STAT5，也称为信号转导和转录激活因子5A和5B（STAT5A/B）。STAT5单克隆抗体（A-9）针对人类STAT5a的氨基酸区域661-794，可有效识别小鼠、大鼠和人类物种中的STAT5a和STAT5b。抗STAT5抗体（A-9）适用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）。STAT5 (A-9) 抗体有非结合型和多种结合型，如辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种 Alexa Fluor® 结合物。STAT5蛋白通过受体酪氨酸激酶或G蛋白偶联受体发生磷酸化，在调节细胞对细胞因子和生长因子的反应中起着关键作用。激活后，STAT5a和STAT5b形成同源或异源二聚体，并转移到细胞核中，启动与细胞增殖、分化、凋亡相关的基因转录，特别是在淋巴造血细胞中。这种信号传导对于T细胞受体信号传导、乳腺发育以及与性二态性相关的肝脏基因表达调控等过程至关重要。STAT5异常活性与多种疾病有关，包括急性早幼粒细胞白血病，其中STAT5b可与视黄酸受体α基因融合。STAT5 (A-9) 单克隆抗体适用于研究STAT5介导的信号传导通路及其对健康和疾病的影响的科学家。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Stat5 抗体 (A-9) 参考文献:

  1. Stat5a和Stat5b在IL-2家族细胞因子信号传导中的作用  |  Lin, JX. and Leonard, WJ. 2000. Oncogene. 19: 2566-76. PMID: 10851055
  2. Stat5a/b有助于白细胞介素7诱导的B细胞前体扩增，但abl-和bcr/abl-诱导的转化与stat5无关。  |  Sexl, V., et al. 2000. Blood. 96: 2277-83. PMID: 10979977
  3. 转录因子 STAT5A 是 B 细胞中布鲁顿酪氨酸激酶的底物。  |  Mahajan, S., et al. 2001. J Biol Chem. 276: 31216-28. PMID: 11413148
  4. GH激活的转录信号转导子和激活子5a（STAT5a）和STAT5b的丝氨酸磷酸化：对STAT5转录活性的影响。  |  Park, SH., et al. 2001. Mol Endocrinol. 15: 2157-71. PMID: 11731617
  5. STAT5a 激活介导致癌物质 RhoA 诱导的上皮细胞向间质转化。  |  Benitah, SA., et al. 2003. Mol Biol Cell. 14: 40-53. PMID: 12529425
  6. 特异性抑制Stat5a/b可促进IL-2反应性原代淋巴细胞和肿瘤衍生淋巴细胞的凋亡。  |  Behbod, F., et al. 2003. J Immunol. 171: 3919-27. PMID: 14530308
  7. STAT5A/5B 缺陷小鼠的耐受性丧失和自身免疫性影响多个器官  |  Snow, JW., et al. 2003. J Immunol. 171: 5042-50. PMID: 14607901
  8. STAT5A 与μ-阿片受体相互作用，并在激活后磷酸化。  |  Mazarakou, G. and Georgoussi, Z. 2005. J Neurochem. 93: 918-31. PMID: 15857395
  9. ERBB4/HER4 通过调节新型 STAT5A 丝氨酸磷酸化事件来增强 STAT5A 的转录活性。  |  Clark, DE., et al. 2005. J Biol Chem. 280: 24175-80. PMID: 15863494
  10. Stat5a/b 对正常淋巴细胞的发育和分化至关重要。  |  Yao, Z., et al. 2006. Proc Natl Acad Sci U S A. 103: 1000-5. PMID: 16418296
  11. STAT5参与肿瘤发生。  |  Halim, CE., et al. 2020. Biomedicines. 8: PMID: 32872372
  12. STAT5事实。  |  Dempsey, LA. 2023. Nat Immunol. 24: 1. PMID: 36596897

订购信息

产品名称产品编号规格价格数量收藏夹

Stat5 抗体 (A-9)

sc-74442
200 µg/ml
RMB2377.00

Stat5 (A-9): m-IgG Fc BP-HRP 套装

sc-527020
200 µg Ab; 10 µg BP
RMB2662.00

Stat5 (A-9): m-IgG1 BP-HRP 套装

sc-532393
200 µg Ab; 20 µg BP
RMB2662.00

Stat5 抗体 (A-9) X

sc-74442 X
200 µg/0.1 ml
RMB2377.00

Stat5 抗体 (A-9) AC

sc-74442 AC
500 µg/ml, 25% agarose
RMB3129.00

Stat5 抗体 (A-9) HRP

sc-74442 HRP
200 µg/ml
RMB2377.00

Stat5 抗体 (A-9) FITC

sc-74442 FITC
200 µg/ml
RMB2482.00

Stat5 抗体 (A-9) PE

sc-74442 PE
200 µg/ml
RMB2580.00

Stat5 抗体 (A-9) Alexa Fluor® 488

sc-74442 AF488
200 µg/ml
RMB2685.00

Stat5 抗体 (A-9) Alexa Fluor® 546

sc-74442 AF546
200 µg/ml
RMB2685.00

Stat5 抗体 (A-9) Alexa Fluor® 594

sc-74442 AF594
200 µg/ml
RMB2685.00

Stat5 抗体 (A-9) Alexa Fluor® 647

sc-74442 AF647
200 µg/ml
RMB2685.00

Stat5 抗体 (A-9) Alexa Fluor® 680

sc-74442 AF680
200 µg/ml
RMB2685.00

Stat5 抗体 (A-9) Alexa Fluor® 790

sc-74442 AF790
200 µg/ml
RMB2685.00

I am going to perform some IHC on my mouse tissues. How can I do this with a mouse monoclonal primary?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. For immunostaining, consider using sc-271542 which is available conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 13
Date published: 2017-01-11

I would like to perform a double stain. How can I do this with only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. For a double stain, consider using sc-271542 which is available conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 13
Date published: 2017-01-11
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Rated 5 out of 5 by from Great antibody!Great antibody for probing STAT5 via WB. Used in mouse lysates with great results. Gave nice strong bands.
Date published: 2018-03-23
Rated 5 out of 5 by from Normal workI brought STAT6（A9）last month and it worked normal.
Date published: 2018-01-21
Rated 5 out of 5 by from Stat5 reviewI used the Stat5 (A-9) antibody (1:250 dilution) for Western blotting and was able to detect a good signal.
Date published: 2017-08-25
Rated 5 out of 5 by from worked wellI used it for western blot on HeLa whole cell lysates, bands came out very clear.
Date published: 2017-08-17
Rated 5 out of 5 by from good antibody for WBWe used this antibody in WB in human tissue and clean, recommend to buy this product.
Date published: 2017-05-09
Rated 5 out of 5 by from Outstanding IHC and WBGreat product for multiple applications; better than polyclonal alternative
Date published: 2017-01-13
Rated 5 out of 5 by from Awesome for mouse, rat, or human detectionAntibody has produced excellent results in Western blot with mouse, rat, and human samples.
Date published: 2016-12-20
Rated 5 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody, good specificity and strong signal strength in K-562 whole cell lysate and K-562 nuclear extract. -SCBT QC
Date published: 2015-03-04
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Stat5 抗体 (A-9) is rated 5.0 out of 5 by 10.
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