Date published: 2025-9-6
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Stat4 Antibody (C-4) - Western Blotting - Image 378474
Stat4 抗体 (C-4): sc-398228. 对 HeLa (A), ACHN (B), NCI-H929 (C), U266 (D), MOLT-4 (E) 和 TK-1 (F) 全细胞裂解液中 Stat4 表达的 Western 印迹分析.
Stat4 抗体 (C-4): sc-398228. 对 HeLa (A), ACHN (B), NCI-H929 (C), U266 (D), MOLT-4 (E) 和 TK-1 (F) 全细胞裂解液中 Stat4 表达的 Western 印迹分析.

Stat4 抗体 (C-4): sc-398228

4.7(7)
寫評論提問

说明书
  • Stat4 抗体 C-4 是小鼠单克隆 IgG2b κ，Stat4抗体, 在8篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的Stat4的N-terminus的氨基酸10-31之间
  • Stat4 抗体 (C-4) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的Stat4; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗Stat4抗体（C-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-398228 X, 200 µg/0.1 ml)
  • 关于如何获取Stat4 (C-4): sc-398228的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是Stat4 Antibody (C-4) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Stat4 Antibody (C-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Stat抗体，包括Stat1, Stat2, Stat3, Stat4, Stat5和Stat6

Stat4抗体（C-4）是一种IgG2b κ小鼠单克隆Stat4抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Stat4蛋白。Stat4抗体（C-4）既可以是非偶联抗Stat4抗体形式提供，也可以是多种偶联形式的抗Stat4抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。各种配体（包括干扰素和生长激素，如EGF）通过膜受体信号传导诱导Jak激酶的激活，从而导致各种Stat转录因子的酪氨酸磷酸化。Stat1和Stat2由IFN-α诱导，形成异二聚体，是ISGF3转录因子复合物的一部分。虽然早期报告表明Stat3被EGF和IL-6激活，但已经证明Stat3β似乎被两者激活，而Stat3α被EGF激活，但不被IL-6激活。Stat4在睾丸和髓样细胞中表达最高。IL-12已被确定为Stat4的激活剂。Stat5已被证明被催乳素和IL-3激活。Stat6参与IL-4激活的信号通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Stat4 抗体 (C-4) 参考文献:

  1. 用反义硫代磷酸寡核苷酸靶向转录因子 STAT4 可抑制胶原蛋白诱发的关节炎。  |  Hildner, KM., et al. 2007. J Immunol. 178: 3427-36. PMID: 17339437
  2. FRS2家族对接/支架适配蛋白对生长因子信号传导的调节。  |  Korman, BD., et al. 2008. Curr Allergy Asthma Rep. 8: 398-403. PMID: 18682104
  3. 成纤维细胞生长因子受体酪氨酸激酶对SNT1的细胞和受体同型特异性磷酸化。  |  Wu, S., et al. 2018. Biosci Rep. 38: PMID: 30054428
  4. FRS2家族对接/支架适配蛋白作为癌症治疗的治疗靶点。  |  Grassmann, S., et al. 2023. bioRxiv.. PMID: 37986752
  5. 酪氨酸磷酸化的 Stat1 和 Stat2 加上一个 48 kDa 蛋白都与 DNA 接触，形成干扰素刺激基因因子 3。  |  Qureshi, SA., et al. 1995. Proc Natl Acad Sci U S A. 92: 3829-33. PMID: 7537377
  6. Jun 和 Stat3 的一种简称 Stat3 beta 的合作转录活性。  |  Schaefer, TS., et al. 1995. Proc Natl Acad Sci U S A. 92: 9097-101. PMID: 7568080
  7. 对多肽配体的转录反应：JAK-STAT 途径。  |  Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
  8. 白细胞介素-3, 促红细胞生成素和催乳素可激活淋巴细胞中的 STAT5 类因子。  |  Pallard, C., et al. 1995. J Biol Chem. 270: 15942-5. PMID: 7608147
  9. Stat4，一种在早期骨髓分化过程中表达的新型γ干扰素激活位点结合蛋白。  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
  10. 白细胞介素-4 诱导的转录因子：IL-4 Stat.  |  Hou, J., et al. 1994. Science. 265: 1701-6. PMID: 8085155
  11. Stat3：一种 STAT 家族成员，在表皮生长因子和白细胞介素-6 的作用下通过酪氨酸磷酸化激活。  |  Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
  12. Jak-STAT 通路和转录激活对 IFNs 及其他细胞外信号蛋白的反应。  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

订购信息

产品名称产品编号规格价格数量收藏夹

Stat4 抗体 (C-4)

sc-398228
200 µg/ml
RMB2377.00

Stat4 (C-4): m-IgG Fc BP-HRP 套装

sc-526299
200 µg Ab; 10 µg BP
RMB2662.00

Stat4 (C-4): m-IgG2b BP-HRP 套装

sc-549710
200 µg Ab; 10 µg BP
RMB2662.00

Stat4 抗体 (C-4) X

sc-398228 X
200 µg/0.1 ml
RMB2377.00

Stat4 抗体 (C-4) AC

sc-398228 AC
500 µg/ml, 25% agarose
RMB3129.00

Stat4 抗体 (C-4) HRP

sc-398228 HRP
200 µg/ml
RMB2377.00

Stat4 抗体 (C-4) FITC

sc-398228 FITC
200 µg/ml
RMB2482.00

Stat4 抗体 (C-4) PE

sc-398228 PE
200 µg/ml
RMB2580.00

Stat4 抗体 (C-4) Alexa Fluor® 488

sc-398228 AF488
200 µg/ml
RMB2685.00

Stat4 抗体 (C-4) Alexa Fluor® 546

sc-398228 AF546
200 µg/ml
RMB2685.00

Stat4 抗体 (C-4) Alexa Fluor® 594

sc-398228 AF594
200 µg/ml
RMB2685.00

Stat4 抗体 (C-4) Alexa Fluor® 647

sc-398228 AF647
200 µg/ml
RMB2685.00

Stat4 抗体 (C-4) Alexa Fluor® 680

sc-398228 AF680
200 µg/ml
RMB2685.00

Stat4 抗体 (C-4) Alexa Fluor® 790

sc-398228 AF790
200 µg/ml
RMB2685.00

Stat4 (C-4) 中和勝肽

sc-398228 P
100 µg/0.5 ml
RMB511.00

Does this antibody recognize only unphosphorylated STAT4, or does it recognize all STAT4 regardless of phosphorylation status?

Asked by: trash3
Thank you for your question. This antibody recognizes total Stat4 regardless of phosphorylation status.
Answered by: Technical Support
Date published: 2019-08-17

What application is the blocking peptide sc-398228 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good Ab!I tried this monoclonal Ab in Jurkat and MEC-1 cytoplasmic cell lysates. After overnight incubation with a 1:500 dilution, I detected a single band at ~75 kDa.
Date published: 2023-09-18
Rated 5 out of 5 by from Stat4效果杠杠滴一个月前我申购了这支抗体，才跑了2次WB条带就出来了，虽然有非特异性条带，总体来说还是很不错的，到货速度也是相当快
Date published: 2017-10-23
Rated 5 out of 5 by from Great featureThis antibody was diluted in milk (1:500). One specific band of stat4 was detected. I didn't observe multiple bands as catalog photos.
Date published: 2017-08-10
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of Stat4 expression in Jurkat and HeLa whole cell lysates.
Date published: 2017-02-06
Rated 4 out of 5 by from Very good antibody for Western blottingThis product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and possibly highly specific antibody for Western blotting with lysates from diverse human cancer cell lines. In immunoblots with a mix of HeLa and Jurkat cell lysates, this antibody strongly detected a ~70 kDa protein, which is smaller that the expected size of human Stat4, which is predicted to be ~86 kDa. Other commercial STAT4 antibodies tend to support a ~90 kDa size for STAT4 on Western blots.
Date published: 2016-10-13
Rated 5 out of 5 by from Works very nicely for IF on mouse NIH/3T3 samplesWorks very nicely for IF on mouse NIH/3T3 samples. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody, worked well with CTLL-2 whole cell lysates.
Date published: 2015-02-26
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Stat4 抗体 (C-4) is rated 4.7 out of 5 by 7.
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