Date published: 2025-9-7
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Sox9 Antibody (E-9) - Western Blotting - Image 384304
Sox-9 抗体 (E-9) HRP: sc-166505 HRP. 非转染: sc-117752 (A), 人 Sox-9 转染: sc-126032 (B) 全细胞裂解液和 SW480 核提取物 (C) 中 Sox-9 表达的直接 Western 印迹分析.
Sox-9 抗体 (E-9) HRP: sc-166505 HRP. 非转染: sc-117752 (A), 人 Sox-9 转染: sc-126032 (B) 全细胞裂解液和 SW480 核提取物 (C) 中 Sox-9 表达的直接 Western 印迹分析.

Sox9 抗体 (E-9): sc-166505

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寫評論提問

说明书
  • Sox9 抗体 E-9 是小鼠单克隆 IgG2a κ，Sox9抗体, 在79篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Sox-9的N-terminus附近的氨基酸36-55之间
  • Sox9 抗体 (E-9) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 avian来源的Sox-9; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗Sox9抗体（E-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-166505 X, 200 µg/0.1 ml)
  • 关于如何获取Sox9 (E-9): sc-166505的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Sox9 Antibody (E-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Sox9 Antibody (E-9) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Sox抗体，包括Sox-10, Sox-13, Sox-15, Sox-17, Sox-18, Sox-2, Sox-21, Sox-22, Sox-3, Sox-30, Sox-4, Sox-5, Sox-6, Sox-8和Sox-9

Sox-9 抗体（E-9）是一种 IgG2a κ 小鼠单克隆 Sox-9 抗体（也称为 SOX9 抗体或 SRY 相关 HMG 盒转录因子 9 抗体），可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人类的 Sox-9 蛋白。Sox-9 抗体（E-9）既可以是非偶联的抗 Sox-9 抗体形式，也可以是多种偶联形式的抗 Sox-9 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。Sox 基因是与哺乳动物性别决定基因 SRY 相关的一组基因。这些基因同样包含编码 HMG 盒结构域的序列，该结构域负责序列特异性的 DNA 结合活性。Sox 基因编码推定的转录调节因子，这些因子参与发育过程中细胞命运的决定和多种发育过程的控制。高度复杂的 Sox 基因群至少聚集了 40 个不同的位点，这些位点在各种动物谱系中迅速分化。目前，已经鉴定出 30 个 Sox 基因。已经证明，这个家族的成员在进化过程中是保守的，并在动物发育过程中发挥关键作用。其中一些与包括性逆转在内的人类疾病有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Sox9 抗体 (E-9) 参考文献:

  1. 鉴定新型 Sry 相关基因及其生殖细胞特异性表达。  |  Osaki, E., et al. 1999. Nucleic Acids Res. 27: 2503-10. PMID: 10359848
  2. Sox 因子在神经决定过程中的作用：进化中的保守信号传递？  |  Sasai, Y. 2001. Int J Dev Biol. 45: 321-6. PMID: 11291862
  3. HMG 盒超家族的起源和多样性。  |  Laudet, V., et al. 1993. Nucleic Acids Res. 21: 2493-501. PMID: 8506143
  4. 神经胶质细胞中的新型转录调节器 Sox10  |  Kuhlbrodt, K., et al. 1998. J Neurosci. 18: 237-50. PMID: 9412504
  5. 人类 SOX14 基因的特征和图谱。  |  Arsic, N., et al. 1998. Cytogenet Cell Genet. 83: 139-46. PMID: 9925951

订购信息

产品名称产品编号规格价格数量收藏夹

Sox9 抗体 (E-9)

sc-166505
200 µg/ml
RMB2377.00

Sox9 (E-9): m-IgG Fc BP-HRP 套装

sc-528983
200 µg Ab; 10 µg BP
RMB2662.00

Sox9 (E-9): m-IgGκ BP-HRP 套装

sc-521635
200 µg Ab, 40 µg BP
RMB2662.00

Sox9 (E-9): m-IgG2a BP-HRP 套装

sc-547225
200 µg Ab; 10 µg BP
RMB2662.00

Sox9 抗体 (E-9) X

sc-166505 X
200 µg/0.1 ml
RMB2377.00

Sox9 抗体 (E-9) AC

sc-166505 AC
500 µg/ml, 25% agarose
RMB3129.00

Sox9 抗体 (E-9) HRP

sc-166505 HRP
200 µg/ml
RMB2377.00

Sox9 抗体 (E-9) FITC

sc-166505 FITC
200 µg/ml
RMB2482.00

Sox9 抗体 (E-9) PE

sc-166505 PE
200 µg/ml
RMB2580.00

Sox9 抗体 (E-9) Alexa Fluor® 488

sc-166505 AF488
200 µg/ml
RMB2685.00

Sox9 抗体 (E-9) Alexa Fluor® 546

sc-166505 AF546
200 µg/ml
RMB2685.00

Sox9 抗体 (E-9) Alexa Fluor® 594

sc-166505 AF594
200 µg/ml
RMB2685.00

Sox9 抗体 (E-9) Alexa Fluor® 647

sc-166505 AF647
200 µg/ml
RMB2685.00

Sox9 抗体 (E-9) Alexa Fluor® 680

sc-166505 AF680
200 µg/ml
RMB2685.00

Sox9 抗体 (E-9) Alexa Fluor® 790

sc-166505 AF790
200 µg/ml
RMB2685.00

Sox9 (E-9) 中和勝肽

sc-166505 P
100 µg/0.5 ml
RMB511.00

I would like to know at which dilution this antibody Sox9/647 (sc-166505) is used for immunofluorescence assays. Thanks, Jordi

Asked by: Jordi
Santa Cruz Biotechnology tests at a 1:50 dilution for Immunofluorescence.
Answered by: Technical Service
Date published: 2024-05-07

Is this product appropriate for flow cytometry? Have you got citations of people successfully using it?

Asked by: ailsaw
Thank you for your question. Fluorescent conjugates of this antibody can be used for flow cytometry. As of yet, we have not received any published data on flow cytometry use.
Answered by: Tech Support Europe
Date published: 2023-09-22

where is the band area， why mine is at 40~，

Asked by: minch
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Jiajun Z
Date published: 2023-07-27

Can the conjugated version of this antibody (546) be used in combination with other secondary antibodies for double-staining? I haven't used many mouse-on-mouse stains and I know that can be problematic but mouse is listed under reactive species.

Asked by: Anonymous
Thank you for your question. We do not recommend using a secondary antibody or binding protein with a primary antibody that is already conjugated. Steric hindrance may negatively influence your results. Most samples and tissues are unproblematic when using a mouse primary antibody and anti-mouse secondary detection. When working with tissues make sure to remove all blood from the sample to prevent the secondary from picking up endogenous IgG. Using a direct conjugate (without a secondary) will circumvent this issue entirely.
Answered by: Technical Support Europe
Date published: 2020-10-28

Has anyone tried with IHC before? I have got mice testes samples which are paraffin fixed. I tried doing it but there isn't any signal.

Asked by: Evaa
Thank you for your question. We have not yet tested this antibody in formalin-fixed paraffin-embedded tissue sections, and so IHC(P) is not a recommended application at this time. We have successfully used the antibody on formalin-fixed cells for IF. In addition, there are several publications citing use of this antibody for IHC staining. For example, PMID's: 25917841 and 30244259.
Answered by: Technical Support
Date published: 2019-02-26

Is this product acceptable for ChIP/ChIP-seq applications?

Asked by: che183
This antibody has not yet been tested in the ChIP or ChIP-seq applications.
Answered by: Technical Support
Date published: 2018-12-21

I used this antibody for IF in rat cartilage cryosections but no staining was observed. Could you please recommend a protocol for this? Which is the best fixative and permeabilization agent? Is antigen retrieval necessary? Thanks.

Asked by: sox9
Thank you for your question. I have provided a link to our recommended protocol for immunofluorescent staining of frozen tissue sections. We recommend fixing slides in cold acetone for 10 minutes. A permeabilization agent should not be necessary if fixing in acetone. If you are using a formalin-based fixative, then permeabilization with Triton X-100 may be needed. I would also recommend reviewing the methods in the following publication on PubMed (PMID: 25917841) which cites use of this Sox-9 Antibody (E-9): sc-166505 for IHC in rat tissue. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-01-13

What is the recommended fixation method for IF with this Sox-9 (E-9): sc-166505?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 1 out of 5 by from Poor astrocyte specificityUsed this antibody for astrocyte-specific labeling and it labeled both neurons and astrocytes. Very bad specificity. IHC 1:200 and 1:1000 concentrations, both had very bad astrocyte labeling.
Date published: 2025-03-13
Rated 1 out of 5 by from Did not work well for WBThis Ab did not work well for neonatal mouse lungs for WB.
Date published: 2024-12-10
Rated 5 out of 5 by from First class service.Express delivery, proper documentation, perfect quality.
Date published: 2022-07-28
Rated 5 out of 5 by from 功能很出色，发货速度快，技术支持很给力！客服答复很快，供货稳定，发货速度快，说明很详细，使用效果很好，技术支持也很给力！会推荐给同事，后期需要还会购买！
Date published: 2022-07-04
Rated 5 out of 5 by from CorrectoFunciona correctamente, dentro de los estandares establecidos
Date published: 2021-08-23
Rated 4 out of 5 by from 不错产品不错, 值得下一次购买哦.....................................
Date published: 2021-08-09
Rated 5 out of 5 by from SOX9 antibodyExcellent antibody for immunofluorescence and immunohistochemistry. Dilution: 1 to 1000. Tested in intestinal tissue
Date published: 2021-03-18
Rated 3 out of 5 by from IF(free sample) used for IF for characterization of tumor cells, No problem for signal detection
Date published: 2020-10-06
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Sox9 抗体 (E-9) is rated 4.1 out of 5 by 34.
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