Date published: 2025-9-6
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Sox10 Antibody (A-2) - Western Blotting - Image 391493
Sox-10 抗体 (A-2) Alexa Fluor® 488: sc-365692 AF488. SK-MEL-24 (A), A-375 (B) 和 C6 (C) 全细胞裂解液及人前列腺组织提取物 (D) 中 Sox-10 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Sox-10 抗体 (A-2) Alexa Fluor® 488: sc-365692 AF488. SK-MEL-24 (A), A-375 (B) 和 C6 (C) 全细胞裂解液及人前列腺组织提取物 (D) 中 Sox-10 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Sox10 抗体 (A-2): sc-365692

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说明书
  • Sox10抗体(A-2)是小鼠单克隆IgG1 κ, 在96篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Sox-10的N-terminus的氨基酸2-29之间
  • 抗-Sox10 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Sox-10; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 抗Sox10抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-365692 X, 200 µg/0.1 ml)
  • 关于如何获取Sox10 (A-2): sc-365692的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Sox10 抗体 (A-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Sox10 抗体 (A-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Sox 抗体 包括 Sox-10, Sox-13, Sox-15, Sox-17, Sox-18, Sox-2, Sox-21, Sox-22, Sox-3和Sox-30

Sox-10抗体（A-2）是一种IgG1 κ小鼠单克隆Sox-10抗体（也指定为Sox-10抗体或SOX10抗体），可通过WB（蛋白质印迹）、IP（免疫沉淀）、IF（免疫荧光）、IHC(P)（免疫组织化学，石蜡切片）和ELISA（酶联免疫吸附测定）等方法检测来自小鼠、大鼠和人类的Sox-10。Sox-10抗体（A-2）可以以非偶联的抗Sox-10抗体形式提供，也有多种偶联形式的抗Sox-10抗体，包括琼脂糖、HRP（辣根过氧化物酶）、PE（藻红蛋白）、FITC（异硫氰酸荧光素）和多种Alexa Fluor®偶联物。Sox基因是与哺乳动物性别决定基因SRY相关的一组基因。这些基因同样包含编码HMG-box结构域的序列，该结构域负责序列特异性的DNA结合活性。Sox基因编码推定的转录调节因子，这些调节因子在发育过程中决定细胞命运并控制各种发育过程。高度复杂的Sox基因群至少聚集了40个不同的位点，这些位点在各种动物谱系中迅速分化。目前，已经确定了30个Sox基因。这个家族的成员在进化过程中是保守的，并在动物发育过程中发挥着关键作用。其中一些与人类疾病有关，包括性反转。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Sox10 抗体 (A-2) 参考文献:

  1. 鉴定新型 Sry 相关基因及其生殖细胞特异性表达。  |  Osaki, E., et al. 1999. Nucleic Acids Res. 27: 2503-10. PMID: 10359848
  2. Sox 因子在神经决定过程中的作用：进化中的保守信号传递？  |  Sasai, Y. 2001. Int J Dev Biol. 45: 321-6. PMID: 11291862
  3. HMG 盒超家族的起源和多样性。  |  Laudet, V., et al. 1993. Nucleic Acids Res. 21: 2493-501. PMID: 8506143
  4. 神经胶质细胞中的新型转录调节器 Sox10  |  Kuhlbrodt, K., et al. 1998. J Neurosci. 18: 237-50. PMID: 9412504
  5. 人类 SOX14 基因的特征和图谱。  |  Arsic, N., et al. 1998. Cytogenet Cell Genet. 83: 139-46. PMID: 9925951

订购信息

产品名称产品编号规格价格数量收藏夹

Sox10 抗体 (A-2)

sc-365692
200 µg/ml
RMB2377.00

Sox10 (A-2): m-IgG Fc BP-HRP 套装

sc-527392
200 µg Ab; 10 µg BP
RMB2662.00

Sox10 (A-2): m-IgG1 BP-HRP 套装

sc-532765
200 µg Ab; 20 µg BP
RMB2662.00

Sox10 抗体 (A-2) X

sc-365692 X
200 µg/0.1 ml
RMB2377.00

Sox10 抗体 (A-2) AC

sc-365692 AC
500 µg/ml, 25% agarose
RMB3129.00

Sox10 抗体 (A-2) HRP

sc-365692 HRP
200 µg/ml
RMB2377.00

Sox10 抗体 (A-2) FITC

sc-365692 FITC
200 µg/ml
RMB2482.00

Sox10 抗体 (A-2) PE

sc-365692 PE
200 µg/ml
RMB2580.00

Sox10 抗体 (A-2) Alexa Fluor® 488

sc-365692 AF488
200 µg/ml
RMB2685.00

Sox10 抗体 (A-2) Alexa Fluor® 546

sc-365692 AF546
200 µg/ml
RMB2685.00

Sox10 抗体 (A-2) Alexa Fluor® 594

sc-365692 AF594
200 µg/ml
RMB2685.00

Sox10 抗体 (A-2) Alexa Fluor® 647

sc-365692 AF647
200 µg/ml
RMB2685.00

Sox10 抗体 (A-2) Alexa Fluor® 680

sc-365692 AF680
200 µg/ml
RMB2685.00

Sox10 抗体 (A-2) Alexa Fluor® 790

sc-365692 AF790
200 µg/ml
RMB2685.00

Sox10 (A-2) 中和勝肽

sc-365692 P
100 µg/0.5 ml
RMB511.00

good afternoon, I am currently working in Dublin, Ireland; I wanted to try your sox10 488 conjugated antibody that think it might suit my exp.s. Are there any small samples that I can acquire to check if it will goes well? sc365692.

Asked by: CrisC
Thank you for your question. Please contact technical service directly to arrange for free antibody samples.
Answered by: Tech Support Europe
Date published: 2021-04-28

Buenos días, Hemos visto en su web que ofrecer la posibilidad de enviar GRATIS 10 µg de muestra de Sox-10 (A-2): sc-365692. Para solicitar esa oferta ¿qué habría que hacer? Muchas gracias, un saludo

Asked by: Anonymous
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-01-21

Please what is the molecular weight of gelatin used in the buffer (0.1%)?

Asked by: Sara94
Thank you for your question. The molecular weight of gelatin is 150-160 kDa.
Answered by: Tech Service
Date published: 2019-10-08

What is the best secondary to use with this antibody in Western Blot?

Asked by: jenni
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-04
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Rated 1 out of 5 by from SOX10 790 abPurchased SOX10 790 for a flow panel. Antibody gives inconsistent results. Purchased from another company with success. I suspect there are issues with this antibody binding to native protein. Did not try on western blot.
Date published: 2023-10-11
Rated 4 out of 5 by from buon funzionamentoAbbiamo usato questo anticorpo al FACS, marcando cellule di melanoma, e abbiamo ottenuto uno staining chiaro e definito.
Date published: 2022-10-07
Rated 5 out of 5 by from sc-365692Worked well with staining. Highly recommend this antibody.
Date published: 2020-10-27
Rated 5 out of 5 by from Great for western blotIt gives one specific band in western blot at the expected molecular size in melanoma cells
Date published: 2020-09-18
Rated 5 out of 5 by from Excellent for immunohistochemistryUsed it 1:100 on canine FFPE tissues. Antigen retrieval with Citrate buffer 6.0 pH. Nice nuclear reaction with no aspecific staining.
Date published: 2020-01-24
Rated 5 out of 5 by from IHC(P) in mouseExcellent immunoperoxidase staining of formalin-fixed paraffin-embedded mouse tissue
Date published: 2019-02-07
Rated 1 out of 5 by from No real staining in tissue, nor in culturesI bought this Ab at the end of the last year. The polyclonal discontinued one (A20) was successfully used for staining in mouse enteric nervous system using PFA fixed whole mounts of intestine or colon and in enteric neuronal/glial cultures - as reported throughout literature. However, in my hands, I have not got a single stained cell in tissue and only autofluorescent cells are positive in culture. I have not tried in brain tissue, it might be that this improved one is not working for enteric nervous system.
Date published: 2019-01-06
Rated 4 out of 5 by from Buon funzionamentoAbbiamo utilizzato su tumore cerebrale umano fissato in Carnoy (fissativo alcolico) e ci siamo trovati bene. Necessita smascheramento antigenico in tampone ad alto pH.
Date published: 2018-12-10
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Sox10 抗体 (A-2) is rated 4.0 out of 5 by 24.
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