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Superoxide Dismutase 1/SOD1 Antibody (24) - Western Blotting - Image 387971
SOD-1 抗体 (24) HRP: sc-101523 HRP. HEK293T (A), Hep G2 (B), DU 145 (C), MCF7 (D), HeLa (E) 和 U266 (F) 全细胞裂解液中 SOD-1 表达的直接 Western 印迹分析.
SOD-1 抗体 (24) HRP: sc-101523 HRP. HEK293T (A), Hep G2 (B), DU 145 (C), MCF7 (D), HeLa (E) 和 U266 (F) 全细胞裂解液中 SOD-1 表达的直接 Western 印迹分析.

Superoxide Dismutase 1/SOD1 抗体 (24): sc-101523

4.3(13)
寫評論提問

说明书
  • Superoxide Dismutase 1/SOD1抗体(24)是小鼠单克隆IgG1 κ, 在105篇文献中引用，规格为200 µg/ml
  • 与全长重组蛋白SOD-1（human起源）杂交
  • 抗-Superoxide Dismutase 1/SOD1 抗体 (24) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SOD-1
  • 抗Superoxide Dismutase 1/SOD1抗体（24）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Superoxide Dismutase 1/SOD1 (24): sc-101523的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Superoxide Dismutase 1/SOD1 抗体 (24) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Superoxide Dismutase 1/SOD1 抗体 (24) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加SOD抗体，包括SOD-1, SOD-2和SOD-3

Superoxide Dismutase 1/SOD1 抗体（24）是一种 IgG1 κ 小鼠单克隆 SOD-1 抗体（也称为 SOD-1 抗体），可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人类的 SOD-1。Superoxide Dismutase 1/SOD1 抗体（24）既可以是非偶联的抗 SOD-1 抗体形式，也可以是多种偶联形式的抗 SOD-1 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。Cu-Zn 超氧化物歧化酶-1（SOD-1）是一种特征明确的胞质内氧自由基清除剂，需要铜和锌的结合以增强其酶活性。在酶促反应中，SOD-1 促进氧自由基歧化为过氧化氢，并催化促氧化反应，这包括过氧化物酶活性和羟基自由基生成活性。SOD-1 在体细胞中普遍表达，并以同源二聚体的形式发挥作用。编码 SOD-1 的基因缺陷与神经系统疾病的进展有关，包括肌萎缩侧索硬化症（ALS，一种以脊髓运动神经元丧失为特征的神经退行性疾病）、唐氏综合征和阿尔茨海默病。在家族性 ALS 中，SOD-1 的几种突变占主导地位，导致锌结合能力丧失、SOD-1 清除活性下降，并与神经毒性和运动神经元死亡增加相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Superoxide Dismutase 1/SOD1 抗体 (24)

sc-101523
200 µg/ml
RMB2377.00

Superoxide Dismutase 1/SOD1 (24): m-IgGκ BP-HRP 套装

sc-521199
200 µg Ab, 40 µg BP
RMB2662.00

Superoxide Dismutase 1/SOD1 (24): m-IgG1 BP-HRP 套装

sc-533481
200 µg Ab; 20 µg BP
RMB2662.00

Superoxide Dismutase 1/SOD1 抗体 (24) AC

sc-101523 AC
500 µg/ml, 25% agarose
RMB3129.00

Superoxide Dismutase 1/SOD1 抗体 (24) HRP

sc-101523 HRP
200 µg/ml
RMB2377.00

Superoxide Dismutase 1/SOD1 抗体 (24) FITC

sc-101523 FITC
200 µg/ml
RMB2482.00

Superoxide Dismutase 1/SOD1 抗体 (24) PE

sc-101523 PE
200 µg/ml
RMB2580.00

Superoxide Dismutase 1/SOD1 抗体 (24) Alexa Fluor® 488

sc-101523 AF488
200 µg/ml
RMB2685.00

Superoxide Dismutase 1/SOD1 抗体 (24) Alexa Fluor® 546

sc-101523 AF546
200 µg/ml
RMB2685.00

Superoxide Dismutase 1/SOD1 抗体 (24) Alexa Fluor® 594

sc-101523 AF594
200 µg/ml
RMB2685.00

Superoxide Dismutase 1/SOD1 抗体 (24) Alexa Fluor® 647

sc-101523 AF647
200 µg/ml
RMB2685.00

Superoxide Dismutase 1/SOD1 抗体 (24) Alexa Fluor® 680

sc-101523 AF680
200 µg/ml
RMB2685.00

Superoxide Dismutase 1/SOD1 抗体 (24) Alexa Fluor® 790

sc-101523 AF790
200 µg/ml
RMB2685.00

I am using SOD-1 (24): sc-101523 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2018-11-15
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Rated 5 out of 5 by from Works well for me at 1:2,000Pretty consistent immunoblotting below my 25kDa marker. Works well. I used 1:2,000. It could be optimized to use less antibody.
Date published: 2025-06-20
Rated 4 out of 5 by from It works wellWe used this antibody in WB, good signal. 1:500 concentration
Date published: 2023-10-23
Rated 5 out of 5 by from Really nice!Very good antibody, great results! Primary: 1:200, Secondary: 1 :4000
Date published: 2022-06-14
Rated 5 out of 5 by from super strongAntibody detects single extremely strong band at around 17kDa in U2OS lysates in WB. Dilution 1:2000. I would recommend higher dilution, like 1:3000-4000
Date published: 2019-10-22
Rated 1 out of 5 by from No nos ha funcionado.Probado en lisados de celulas AR42J, no hemosd etectado ninguna banda.
Date published: 2019-05-09
Rated 5 out of 5 by from Good resultsUsed in PMS from primary neuronal culture lysate, reallly good and reproducible results.
Date published: 2019-03-12
Rated 5 out of 5 by from Very good in WBApplication: Western blotting Dilution 1:1000 Cell lysate of C2C12 Very strong and specific band
Date published: 2018-10-05
Rated 4 out of 5 by from good results in WBGood results in Western Blot, we used it 1:500 in milk
Date published: 2018-05-16
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Superoxide Dismutase 1/SOD1 抗体 (24) is rated 4.4 out of 5 by 14.
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