Date published: 2025-9-8
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SNAI 1 Antibody (E-10) - Western Blotting - Image 292936
SNAI 1 抗体 (E-10): sc-393172. 非转染: sc-117752 (A) 和人 SNAI 1 转染: sc-113766 (B) 293T 全细胞裂解液中 SNAI 1 表达的 Western 印迹分析.
SNAI 1 Antibody (E-10) - Western Blotting - Image 292936
SNAI 1 抗体 (E-10): sc-393172. 非转染: sc-117752 (A) 和人 SNAI 1 转染: sc-113766 (B) 293T 全细胞裂解液中 SNAI 1 表达的 Western 印迹分析.
SNAI 1 抗体 (E-10): sc-393172. 非转染: sc-117752 (A) 和人 SNAI 1 转染: sc-113766 (B) 293T 全细胞裂解液中 SNAI 1 表达的 Western 印迹分析.

SNAI 1 抗体 (E-10): sc-393172

4.4(8)
寫評論提問

说明书
  • SNAI 1抗体(E-10)是小鼠单克隆IgG1 κ, 在35篇文献中引用，规格为200 µg/ml
  • 免疫human物种的SNAI 1的氨基酸21-150
  • SNAI 1 抗体 (E-10) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 SNAI 1
  • 抗SNAI 1抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SNAI 1 (E-10): sc-393172的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成SNAI 1 抗体 (E-10)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

SNAI 抗体

SNAI 1抗体（E-10）是一种IgG1 κ鼠单克隆SNAI 1抗体（也称为SNAI 1抗体或SNAI1抗体），可通过WB、IP、IF和ELISA方法检测人源SNAI 1。SNAI 1抗体（E-10）既可以是非偶联的抗SNAI 1抗体形式，也可以是多种偶联形式的抗SNAI 1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Snail家族发育调控蛋白是一组广泛保守的锌指蛋白，可调节转录，包括哺乳动物蛋白SLUG、SNAI 1、果蝇SNAIL的人类同源蛋白和Smuc。SNAI 1和SLUG在胎盘和成人心脏、肝脏和骨骼肌中表达。SNAI 1和相应的小鼠同源蛋白Sna均含有三个经典锌指和一个非典型锌指，而SLUG包含五个锌指区域和氨基末端的转录抑制结构域，这使得SLUG可以作为基因表达的负调节因子。SLUG涉及人类胚胎中神经嵴细胞的生成和迁移，并有助于肢芽的发育。此外，SLUG还构成了一种细胞抗凋亡转录因子，可有效防止剥夺IL-3的小鼠pro-B细胞凋亡。来自小鼠骨骼肌细胞的Snail相关基因Smuc在骨骼肌和胸腺中高表达，同样可以抑制基因转录。Smuc优先与DNA上的CAGGTG和CACCTG E-box基序（CANNTG）结合，并涉及Smuc羧基末端区域的五个假定DNA结合锌指结构域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

SNAI 1 抗体 (E-10)

sc-393172
200 µg/ml
RMB2377.00

SNAI 1 抗体 (E-10) AC

sc-393172 AC
500 µg/ml, 25% agarose
RMB3129.00

SNAI 1 抗体 (E-10) HRP

sc-393172 HRP
200 µg/ml
RMB2377.00

SNAI 1 抗体 (E-10) FITC

sc-393172 FITC
200 µg/ml
RMB2482.00

SNAI 1 抗体 (E-10) PE

sc-393172 PE
200 µg/ml
RMB2580.00

SNAI 1 抗体 (E-10) Alexa Fluor® 488

sc-393172 AF488
200 µg/ml
RMB2685.00

SNAI 1 抗体 (E-10) Alexa Fluor® 546

sc-393172 AF546
200 µg/ml
RMB2685.00

SNAI 1 抗体 (E-10) Alexa Fluor® 594

sc-393172 AF594
200 µg/ml
RMB2685.00

SNAI 1 抗体 (E-10) Alexa Fluor® 647

sc-393172 AF647
200 µg/ml
RMB2685.00

SNAI 1 抗体 (E-10) Alexa Fluor® 680

sc-393172 AF680
200 µg/ml
RMB2685.00

SNAI 1 抗体 (E-10) Alexa Fluor® 790

sc-393172 AF790
200 µg/ml
RMB2685.00

How much protein do you recommend loading on the gel for Western blot detection using SNAI 1 (E-10): sc-393172?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 8
Date published: 2017-02-08
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Rated 4 out of 5 by from Good antibodiesI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 4 out of 5 by from Bon anticorpsJ'ai reçu cet échantillon le mois dernier et il marche très bien. Il sort à un poids moléculaire un peu plus élevé que prévu. Utilisation au 1/1000 dans du blocking buffer, sur un gel d'acrylamide 12.5%.
Date published: 2018-03-07
Rated 5 out of 5 by from goodNice western blot data of snai 1 expression in hk2 cells and kidney injury tissue at 1:1000 dilute.
Date published: 2017-11-20
Rated 3 out of 5 by from doesn't show clear bandThis antibody doesn't show clear band around 30kd for pancreatic cancer cell samples，when we use 1:1000/1:2000(incubate at 4℃ /gentally shaking overnight ).The experimental conditions of mine maybe need optimized in next time.
Date published: 2017-08-30
Rated 4 out of 5 by from well enougha month age ,I have chance to get a sample sack .100ug protein sample of CRC cells for the westernblot ,48h for this antibody, a thin band was gat.
Date published: 2017-06-16
Rated 5 out of 5 by from Good choice for WB!This monoclonal SNAI 1 Antibody (E-10) is a good choice for Western blotting applications. However, alternative monoclonal, SNAI 1 (G-7): sc-271977 is the preferred choice for immunohistochemistry.
Date published: 2017-01-27
Rated 5 out of 5 by from Great Western blot data in sw480 cellGreat Western blot data in sw480 cell at 1:200,it's perfect.
Date published: 2016-11-07
Rated 5 out of 5 by from Nice Western blot data of SNAI 1 expression in nonNice Western blot data of SNAI 1 expression in non-transfected and human SNAI 1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-02-09
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SNAI 1 抗体 (E-10) is rated 4.4 out of 5 by 8.
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