Date published: 2025-9-7
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SMO/Smoothened Antibody (E-5) - Western Blotting - Image 138377
Smo 抗体 (E-5): sc-166685. HeLa (A), MIA PaCa-2 (B) 和 K-562 (C) 全细胞裂解液中 Smo 表达的 Western 印迹分析.
Smo 抗体 (E-5): sc-166685. HeLa (A), MIA PaCa-2 (B) 和 K-562 (C) 全细胞裂解液中 Smo 表达的 Western 印迹分析.

SMO/Smoothened 抗体 (E-5): sc-166685

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说明书
  • SMO/Smoothened 抗体 E-5 是小鼠单克隆 IgG2a κ，SMO/Smoothened抗体, 在97篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Smo的氨基酸488-787
  • SMO/Smoothened 抗体 (E-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Smo
  • 抗SMO/Smoothened抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取SMO/Smoothened (E-5): sc-166685的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是SMO/Smoothened Antibody (E-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与SMO/Smoothened Antibody (E-5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Smo抗体（E-5）是一种IgG2a κ小鼠单克隆Smo抗体（也称为Smoothened抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Smo蛋白。Smo抗体（E-5）既有非偶联的抗Smo抗体形式，也有多种偶联形式的抗Smo抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Wnt-1或GLI蛋白的过度表达会导致癌症；然而，这种转化的分子基础尚不清楚。Wnt-1和GLI蛋白现在已被置于果蝇hedgehog和patched蛋白哺乳动物同源物的下游信号级联中。果蝇体节极性基因hedgehog（hh）编码一种分泌蛋白，该蛋白似乎在胚胎和成虫盘图案形成中发挥作用。ptc基因，也被鉴定为果蝇体节极性基因，编码跨膜蛋白patched，其在胚胎发育过程中的表达受到精确调控。Hedgehog已被证明通过涉及GLI转录因子的信号级联增强Wnt蛋白家族的表达，而patched作为阻遏物对抗hedgehog的作用。Smoothened（Smo）是一种七次跨膜受体，在许多组织与patched形成复合物，被认为是Hh信号通路的重要组成部分。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SMO/Smoothened 抗体 (E-5) 参考文献:

  1. 对非 NF2 脑膜瘤的基因组分析揭示了 TRAF7、KLF4、AKT1 和 SMO 中的突变。  |  Clark, VE., et al. 2013. Science. 339: 1077-80. PMID: 23348505
  2. 成纤维细胞生长因子18可通过SMO-LATS1-YAP途径减轻肝纤维化和造血干细胞活化。  |  Tong, G., et al. 2022. Pharmacol Res. 178: 106139. PMID: 35202822
  3. 在人类胶质瘤中发现一个扩增的高表达基因。  |  Kinzler, KW., et al. 1987. Science. 236: 70-3. PMID: 3563490
  4. Smo-Shh/Gli信号激活在预防神经和衰老疾病中的作用。  |  Prajapati, A., et al. 2023. Biogerontology. 24: 493-531. PMID: 37097427
  5. 人类脐带间充质干细胞衍生的外泌体通过靶向Hedgehog/SMO信号传导改善糖尿病肾病中的肾纤维化。  |  Zhang, K., et al. 2024. FASEB J. 38: e23599. PMID: 38572590
  6. Shh/Smo的激活足以维持少突胶质细胞前体细胞处于未分化状态，而髓鞘形成和（再）髓鞘化则无需激活。  |  Nocera, S., et al. 2024. Glia. 72: 1469-1483. PMID: 38771121
  7. 影响果蝇体节数目和极性的突变  |  Nüsslein-Volhard, C. and Wieschaus, E. 1980. Nature. 287: 795-801. PMID: 6776413
  8. 不同浓度的声刺猬自体蛋白水解氨基末端裂解产物对底板和运动神经元的诱导作用  |  Roelink, H., et al. 1995. Cell. 81: 445-55. PMID: 7736596
  9. 中枢神经系统外植体中不同腹侧细胞类型的诱导需要19K形式的Sonic hedgehog。  |  Martí, E., et al. 1995. Nature. 375: 322-5. PMID: 7753196
  10. Wnt-1 作为跨膜蛋白的活性。  |  Parkin, NT., et al. 1993. Genes Dev. 7: 2181-93. PMID: 8224845
  11. 基底细胞痣综合征候选基因 patched 的人类同源物。  |  Johnson, RL., et al. 1996. Science. 272: 1668-71. PMID: 8658145
  12. 肿瘤抑制基因 patched 编码音速刺猬的候选受体。  |  Stone, DM., et al. 1996. Nature. 384: 129-34. PMID: 8906787

订购信息

产品名称产品编号规格价格数量收藏夹

SMO/Smoothened 抗体 (E-5)

sc-166685
200 µg/ml
RMB2377.00

SMO/Smoothened (E-5): m-IgG2a BP-HRP 套装

sc-545928
200 µg Ab; 10 µg BP
RMB2662.00

SMO/Smoothened 抗体 (E-5) AC

sc-166685 AC
500 µg/ml, 25% agarose
RMB3129.00

SMO/Smoothened 抗体 (E-5) HRP

sc-166685 HRP
200 µg/ml
RMB2377.00

SMO/Smoothened 抗体 (E-5) FITC

sc-166685 FITC
200 µg/ml
RMB2482.00

SMO/Smoothened 抗体 (E-5) PE

sc-166685 PE
200 µg/ml
RMB2580.00

SMO/Smoothened 抗体 (E-5) Alexa Fluor® 488

sc-166685 AF488
200 µg/ml
RMB2685.00

SMO/Smoothened 抗体 (E-5) Alexa Fluor® 546

sc-166685 AF546
200 µg/ml
RMB2685.00

SMO/Smoothened 抗体 (E-5) Alexa Fluor® 594

sc-166685 AF594
200 µg/ml
RMB2685.00

SMO/Smoothened 抗体 (E-5) Alexa Fluor® 647

sc-166685 AF647
200 µg/ml
RMB2685.00

SMO/Smoothened 抗体 (E-5) Alexa Fluor® 680

sc-166685 AF680
200 µg/ml
RMB2685.00

SMO/Smoothened 抗体 (E-5) Alexa Fluor® 790

sc-166685 AF790
200 µg/ml
RMB2685.00

SMO/Smoothened 抗体 (E-5) B

sc-166685 B
200 µg/ml
RMB2407.00

When I use this antibody in Western blot experiments, I always observe multiple bands. I wonder if this could be related to my denaturation method. I usually add 5× loading buffer to the protein lysate and incubate it at 37°C for 30 minutes. Do you have 

Asked by: pengyu
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Yanru Tao
Date published: 2025-08-08

Is this anti-Smo antibody also availaablve biotinylated ?

Asked by: AMH1
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-06-09

Hello, my lab works with S. purpuratus sea urchins. Could you please check if the antibody will be compatible with our species? This is the NCBI reference sequence for the Smo protein in urchins: XP_030830155.1

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-11

mokey유래의 SMO와 반응할 수 있나요?

Asked by: haetnim
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Has this antibody been tested in Xenopus, either WB or IF?

Asked by: AHamilton
Thank you for your question. Anti-Smo Antibody (E-5) has only been tested for detection of Smo of mouse, rat and human origin by WB, IP, IF and ELISA. It has not been tested in samples from Xenopus.
Answered by: Tech Support Europe
Date published: 2020-09-22

Hello,could you let me know which region of Smo this antibody was raised agianst, extracellular region, intracellular region or...? Thanks.

Asked by: Yin2019
Thank you for your question. This antibody was raised against amino acids 488-787 of Smo of human origin, which spans both cytoplasmic and extracellular regions. The exact epitope has not been epitope mapped. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-03-25

Hi, I actually want to know when this antibody became available on the market. Could you tell me which archived SCBio catalog this product first appeared in and if possible, send me the pdf of that catalog? Thanks!

Asked by: Kay Gee
Thank you for your question. This is an in house monoclonal antibody that we released in 2009. All the information we offer for this antibody is on our website: https://www.scbt.com/scbt/product/smo-antibody-e-5?requestFrom=search Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-06-06

Hello, Could you tell me when the Smo (E-5) product was published in a catalogue and whether you could provide the document with the publication date mentioned on it? Thanks so much!

Asked by: Kay Gee
Thank you for your question. You can find citations using this antibody in the scientific literature at the very bottom of the product page on our website. Follow this link, and scroll to the bottom: https://www.scbt.com/scbt/product/smo-antibody-e-5?requestFrom=search This antibody currently has 8 citations. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-06
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Rated 4 out of 5 by from 量大好用前几个月买了这个抗体，用了一下抗体染色效果不错，而且量大，价格还实惠，值得推荐！一只抗体能发好几篇文章
Date published: 2023-12-29
Rated 5 out of 5 by from 产品很出色！我们不久前购买了此产品，送货速度很快，拿到抗体后立刻做了WB，这个抗体很好用，条带清晰，位置正确，推荐。
Date published: 2023-11-28
Rated 4 out of 5 by from Good for wbWe used Smo (E-5) antibody in western blot to verify Smoothened genetic silencing. This product worked very well. We used a 1:1000 diluition in whole lysate from cholangiocarcinoma cell lines.
Date published: 2023-03-27
Rated 4 out of 5 by from good for WM-IFThis antibody worked good for whole-mount immunofluorescence at 5.0 μg/ml in PLP-fixed mouse embryonic telencephalons.
Date published: 2023-02-21
Rated 4 out of 5 by from Works on Western and IFEndogenous Smoothened expression detected on western using this antibody at 1:100 in ATDC5 and hTERT-RPE1 cells. Overexpression of Smoothened in same cell lines detected on western using 1:1000. Endogenous Smoothened expression detected in IF using this antibody at 1:100 in MEF, NIH3T3, BJ fibroblasts and hTERT-RPE1 (slight background signal).
Date published: 2020-10-30
Rated 3 out of 5 by from Not really goodI tested using 1:500 dilution for western blot in MEF cells. But there has non-specific bands.
Date published: 2019-05-23
Rated 5 out of 5 by from good antibodyit worked well at 1:400 in WB with a very low background
Date published: 2018-11-29
Rated 3 out of 5 by from good for western blot analysisWe used this antibody to detect Smo and got a good result. Fresh liver samples were homogenized in RIPA buffer, then 40ug of total protein were subjected to 10% gel for SDS-PAGE and transferred onto a PVDF membrane. After blocking with 5% skim milk in 1×TBS-T, membrane were incubated overnight at 4℃ with Smo antibodies(1:1000).And then, it were incubated with anti- mouse IgG(1:2000) at room temperature and detected with ECL kit.
Date published: 2017-06-07
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SMO/Smoothened 抗体 (E-5) is rated 4.4 out of 5 by 13.
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