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Smad7 Antibody (B-8) - Western Blotting - Image 145526
Smad7 抗体 (B-8): sc-365846. Western 印迹分析 A549 全细胞裂解液中 Smad7 的表达.
Smad7 Antibody (B-8) - Western Blotting - Image 145526
Smad7 抗体 (B-8): sc-365846. Western 印迹分析 A549 全细胞裂解液中 Smad7 的表达.
Smad7 Antibody (B-8) - Western Blotting - Image 396185
Smad7 抗体 (B-8) Alexa Fluor® 647: sc-365846 AF647. 直接荧光西部印迹分析 A549 全细胞裂解液中 Smad7 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.
Smad7 抗体 (B-8): sc-365846. Western 印迹分析 A549 全细胞裂解液中 Smad7 的表达.

Smad7 抗体 (B-8): sc-365846

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寫評論提問

说明书
  • Smad7 抗体 B-8 是小鼠单克隆 IgG1 κ，Smad7抗体, 在87篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Smad7的氨基酸319-397
  • Smad7 抗体 (B-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Smad7
  • 抗Smad7抗体（B-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-365846 X, 200 µg/0.1 ml)
  • 关于如何获取Smad7 (B-8): sc-365846的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Smad7 Antibody (B-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Smad7 Antibody (B-8) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Smad抗体，包括Smad1, Smad2, Smad3, Smad4, Smad5, Smad6, Smad7和Smad8

Smad7抗体（B-8）是一种IgG1 κ小鼠单克隆Smad7抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Smad7蛋白。Smad7抗体（B-8）既可作为非偶联抗Smad7抗体形式提供，也可作为多种偶联形式的抗Smad7抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Smad蛋白是果蝇抗dpp（Mad）的哺乳动物同源物，已被认为是TGFβ/BMP信号通路的下游效应物。Smad1（也称为Madr1或JV4-1）、Smad5和哺乳动物Smad8（也称为Smad9或MADH6）是BMP2和BMP4功能的效应物，而Smad2（也称为Madr2或JV18-1）和Smad3则参与TGFβ和激活素介导的生长调节。已证明Smad4（也称为DPC4）通过与各种Smad家族成员相互作用来介导上述所有活动。Smad6和Smad7通过干扰TGFβ介导的其他Smad家族成员的磷酸化来调节对激活素/TGFβ信号的反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Smad7 抗体 (B-8) 参考文献:

  1. 对活体小鼠中 Smad2/3 依赖性 TGF-beta 信号传导的全面分析表明，组织对损伤有明显的特异性反应。  |  Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
  2. Smads 和染色质调节  |  van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
  3. 通过 Smad 途径传递 TGF-beta 信号。  |  Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
  4. 一种作为 BMP 调节的转录激活因子的人类 Mad 蛋白。  |  Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
  5. MADR2 位于 18q21，编码一种受 TGFbeta 调节的 MAD 相关蛋白，在结直肠癌中发生功能突变。  |  Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
  6. 与受体相关的 Mad 同源物协同作用，成为 TGF-beta 反应的效应因子。  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
  7. DPC4 和 SMAD 蛋白在 TGF-beta 信号通路中的合作关系。  |  Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
  8. Smad6 可抑制 TGF-beta 超家族的信号传导。  |  Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
  9. Smad8介导ALK-2[校正]受体丝氨酸激酶的信号传导。  |  Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
  10. TGF-beta 信号通过 SMAD 蛋白从细胞膜传递到细胞核。  |  Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997

订购信息

产品名称产品编号规格价格数量收藏夹

Smad7 抗体 (B-8)

sc-365846
200 µg/ml
RMB2377.00

Smad7 (B-8): m-IgG Fc BP-HRP 套装

sc-529491
200 µg Ab; 10 µg BP
RMB2662.00

Smad7 (B-8): m-IgGκ BP-HRP 套装

sc-522394
200 µg Ab, 40 µg BP
RMB2662.00

Smad7 (B-8): m-IgG1 BP-HRP 套装

sc-543541
200 µg Ab; 20 µg BP
RMB2662.00

Smad7 抗体 (B-8) X

sc-365846 X
200 µg/0.1 ml
RMB2377.00

Smad7 抗体 (B-8) AC

sc-365846 AC
500 µg/ml, 25% agarose
RMB3129.00

Smad7 抗体 (B-8) HRP

sc-365846 HRP
200 µg/ml
RMB2377.00

Smad7 抗体 (B-8) FITC

sc-365846 FITC
200 µg/ml
RMB2482.00

Smad7 抗体 (B-8) PE

sc-365846 PE
200 µg/ml
RMB2580.00

Smad7 抗体 (B-8) Alexa Fluor® 488

sc-365846 AF488
200 µg/ml
RMB2685.00

Smad7 抗体 (B-8) Alexa Fluor® 546

sc-365846 AF546
200 µg/ml
RMB2685.00

Smad7 抗体 (B-8) Alexa Fluor® 594

sc-365846 AF594
200 µg/ml
RMB2685.00

Smad7 抗体 (B-8) Alexa Fluor® 647

sc-365846 AF647
200 µg/ml
RMB2685.00

Smad7 抗体 (B-8) Alexa Fluor® 680

sc-365846 AF680
200 µg/ml
RMB2685.00

Smad7 抗体 (B-8) Alexa Fluor® 790

sc-365846 AF790
200 µg/ml
RMB2685.00

Can I ask for a free sample?

Asked by: lyue
Thank you for your inquiry. For a limited time, we are offering free samples. Please contact our Technical Service Department to request a sample, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-01

I've used the Smad7 antibody (sc-365846) and detected two bands about 30kDa and 75kDa. I've examined mouse kidney tissue that was homogenized using RIPA lysis buffer. Do you have information about the two detected bands?

Asked by: AJ27
Thank you for your inquiry and your interest in this product. We have not tested the Smad7 Antibody (B-8): sc-365846 on mouse kidney tissues. The molecular weight of murine mothers against decapentaplegic homolog 7 protein is approximately 46 kDa. We would expect to obtain bands at this size between 45 - 50 kDa on a Western Blot. We do not have any information about the band sizes you described. Please find further information about this protein under the following uniprot link: http://www.uniprot.org/uniprot/O35253 Please contact our European Support Office in Germany directly for further technical assistance. You can reach us by phone at: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com We are happy to assist you.
Answered by: Technical Support Europe
Date published: 2021-07-07

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2017-01-06
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Rated 5 out of 5 by from Works well for WBWorks well for neonatal mouse lungs at the concentration of 30ug of protein. Ab dilution will be 1:250 for overnight at 4oC
Date published: 2024-12-13
Rated 4 out of 5 by from 还行用来几天实验还未完成，等过几天完成实验看到结果再做具体感受，相信还是不错的，小鼠的抗体三塔比较齐，继续使用
Date published: 2021-12-29
Rated 1 out of 5 by from Not appropriate for tissue extractsDidn't work with extracts from skeletal muscles overexpressing human Smad7. I used the recommended titer of 1:100, which is utterly ridiculous, and still detected nothing. Antibodies from other vendors worked (see picture) and I suspect the product has been diluted to the point that it only works with cell culture extracts. This is growing problem in the field and suppliers need to stop doing this; it's better to charge more for a product that works than sell a highly diluted one that doesn't. Seriously, 1:100?!?! SC honored their guarantee though and provided a different antibody for a different protein in "exchange".
Date published: 2021-11-12
Rated 4 out of 5 by from 还不错试用了一下，跑的成纤维细胞的SMAD7，效果还不错，推荐购买。不过我可能是浓度配高了，所以有一些非特异条带出来。
Date published: 2018-02-01
Rated 2 out of 5 by from Not worked in my experimentsTried it in my Wes Protein Simple, did not get any reasonable band. Also tried Immunofluorescence in fibroblasts, not stained. Have not yet tried Western Blot.
Date published: 2017-12-22
Rated 5 out of 5 by from my review for Smad7 (B-8) antibodyI tested a free antibody for Smad7 (B-8) (sc-365846) using mouse fibrotic kidney samples for Western blot. We loaded 80 ug proteins in each slot in the SDS gel. The antibody was diluted in 1:500 in 3% BSA/PBST and incubated overnight with the blot. We can observe a very clear band around 46 kd. We have repeated this experiment several times and used the fresh diluted antibody on the fresh blot.
Date published: 2017-12-18
Rated 4 out of 5 by from niceAntibody recognizes SMAD7. This is an appropriate control for experiments examining smad7
Date published: 2017-11-03
Rated 3 out of 5 by from smad7抗体一个多月前我购买的smad7，第一次没做出来，稀释比例1:1000，第二次我稀释比例1:500，感觉不清楚，上样量40的蛋白。
Date published: 2017-08-28
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Smad7 抗体 (B-8) is rated 3.9 out of 5 by 19.
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