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Smad7 Antibody (Z8-B) - Western Blotting - Image 34512
Smad7 抗体 (Z8-B): sc-101152. 对 HeLa 全细胞裂解液中 Smad7 的表达进行 Western 印迹分析.
Smad7 Antibody (Z8-B) - Western Blotting - Image 34512
Smad7 抗体 (Z8-B): sc-101152. 对 HeLa 全细胞裂解液中 Smad7 的表达进行 Western 印迹分析.
Smad7 Antibody (Z8-B) - Immunohistochemistry - Image 36316
Smad7 抗体 (Z8-B): sc-101152. 福尔马林固定, 石蜡包埋的人胎盘组织的免疫过氧化物酶染色, 显示核定位.
Smad7 抗体 (Z8-B): sc-101152. 对 HeLa 全细胞裂解液中 Smad7 的表达进行 Western 印迹分析.

Smad7 抗体 (Z8-B): sc-101152

4.1(7)
寫評論提問

说明书
  • Smad7抗体(Z8-B)是小鼠单克隆IgG2a κ, 在30篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组Smad7
  • 推荐用于 mouse, rat 和 human 来源的Smad7 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 Smad7 (B-8): sc-365846，可查看有标记的 Smad7 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取Smad7 (Z8-B): sc-101152的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Smad7 抗体 (Z8-B) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Smad7 抗体 (Z8-B) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Smad 抗体 包括 Smad1, Smad2, Smad3, Smad4, Smad5, Smad6, Smad7和Smad8

Smad7抗体（Z8-B）是一种IgG2a κ小鼠单克隆Smad7抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Smad7。Smad7抗体（Z8-B）以非偶联抗Smad7抗体形式提供。Smad蛋白是果蝇抗dpp（Mad）的哺乳动物同源物，已被认为是TGFβ/BMP信号通路的下游效应物。Smad1（也称为Madr1或JV4-1）、Smad5和哺乳动物Smad8（也称为Smad9或MADH6）是BMP2和BMP4功能的效应物，而Smad2（也称为Madr2或JV18-1）和Smad3则参与TGFβ和激活素介导的生长调节。已证明Smad4（也称为DPC4）通过与各种Smad家族成员相互作用来介导上述所有活动。Smad6和Smad7通过干扰TGFβ介导的其他Smad家族成员的磷酸化来调节对激活素/TGFβ信号的反应。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Smad7 抗体 (Z8-B)

sc-101152
100 µg/ml
RMB2504.00

Smad7 (Z8-B): m-IgG Fc BP-HRP 套装

sc-537301
100 µg Ab; 10 µg BP
RMB2662.00

Smad7 (Z8-B): m-IgGκ BP-HRP 套装

sc-534533
100 µg Ab; 40 µg BP
RMB2662.00

Smad7 (Z8-B): m-IgG2a BP-HRP 套装

sc-547971
100 µg Ab; 10 µg BP
RMB2662.00

您好！smad7 (B-8):sc-365846 smad7(28-B):sc-101152 可以领两个免费小样吗？

Asked by: hf123q
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I would like to know what the second blot line mean in the picture of WB(Smad7 抗体 (Z8-B): sc-101152). My result have two blot lines, I can't find the answer in the introduction. thank you a lot.

Asked by: house
Thank you for your question. We have not characterized both bands which were observed in HeLa whole cell lysate when Western blotting with this antibody. The predicted molecular weight of Smad7 is 46 kDa, and other isoforms of similar size have been reported according to Uniprot. https://www.uniprot.org/uniprot/O15105#sequences
Answered by: Technical Support
Date published: 2018-12-14

I would like to use Smad7 Antibody (Z8-B): sc-101152 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-101152 is not available directly conjugated, but we have an alternative Smad7 antibody (sc-365846) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 6
Date published: 2017-01-11

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Smad7 Antibody (Z8-B): sc-101152?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-11
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Rated 4 out of 5 by from Works good in primary hnscc cellsshowed satisfactory immunofluorescence signal in PFA-fixed primary spheroids of HNSCC cell cultures
Date published: 2025-08-19
Rated 3 out of 5 by from Works in chickenThis antibody works by Western blot on chicken embryo lysates at a dilution of 1:200.
Date published: 2018-03-26
Rated 5 out of 5 by from Works well in my ImmunofluorescenceI tried 1:50 in my fibroblasts IF, got strong/reasonable staining. I have not yet tried Western Blot.
Date published: 2017-12-22
Rated 5 out of 5 by from Strong WB and IHC !Very nice product for IHC studies; also clean western
Date published: 2017-01-15
Rated 4 out of 5 by from Works in IHC(P)Positive staining seen in IHC with paraffin-embedded human tissue samples.
Date published: 2016-12-23
Rated 3 out of 5 by from Showed nuclear staining of varying strengthShowed nuclear staining of varying strength in human placenta tissue (FFPE) -SCBT QC
Date published: 2015-02-10
Rated 5 out of 5 by from Excellent detection of Smad7 in human cornealExcellent detection of Smad7 in human corneal fibroblasts -SCBT Publication Review
Date published: 2013-07-02
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Smad7 抗体 (Z8-B) is rated 4.1 out of 5 by 8.
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