Date published: 2025-9-6
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Smad3 Antibody (38-Q) - Western Blotting - Image 34515
Smad3 抗体 (38-Q): sc-101154. 人结肠组织提取物中 Smad3 表达的 Western 印迹分析.
Smad3 Antibody (38-Q) - Western Blotting - Image 34515
Smad3 抗体 (38-Q): sc-101154. 人结肠组织提取物中 Smad3 表达的 Western 印迹分析.
Smad3 Antibody (38-Q) - Immunohistochemistry - Image 36318
Smad3 抗体 (38-Q): sc-101154. 福尔马林固定, 石蜡包埋的人扁桃体组织的免疫过氧化物酶染色, 显示胞质定位.
Smad3 Antibody (38-Q) - Western Blotting - Image 37019
Smad3 抗体 (38-Q): sc-101154. 未转染: sc-117752 (A) 和转染: sc-116400 (B) 293T 全细胞裂解液中 Smad3 表达的 Western 印迹分析.
Smad3 抗体 (38-Q): sc-101154. 人结肠组织提取物中 Smad3 表达的 Western 印迹分析.

Smad3 抗体 (38-Q): sc-101154

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说明书
  • Smad3抗体(38-Q)是小鼠单克隆IgG2a κ, 在129篇文献中引用，规格为50 µg/0.5 ml
  • 免疫human物种的重组Smad3
  • 推荐用于 mouse, rat 和 human 来源的Smad3 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 Smad2/3 (C-8): sc-133098，可查看有标记的 Smad3 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取Smad3 (38-Q): sc-101154的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Smad3 抗体 (38-Q) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Smad3 抗体 (38-Q) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

额外Smad抗体，包括Smad1, Smad2, Smad3, Smad4, Smad5, Smad6, Smad7和Smad8

Smad3抗体（38-Q）是一种IgG2a κ小鼠单克隆Smad3抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Smad3。Smad3抗体（38-Q）为非偶联的抗Smad3抗体形式。Smad蛋白是果蝇Mothers against dpp（Mad）的哺乳动物同源物，已被证实为TGFβ/BMP信号的下游效应器。Smad1（也称为Madr1或JV4-1）、Smad5和哺乳动物Smad8（也称为Smad9或MADH6）是BMP2和BMP4功能的效应器，而Smad2（也称为Madr2或JV18-1）和Smad3则参与TGFβ和激活素介导的生长调节。已证实Smad4（也称为DPC4）通过与各种Smad家族成员相互作用来介导上述所有活动。Smad6和Smad7通过干扰TGFβ介导的其他Smad家族成员的磷酸化来调节对激活素/TGFβ信号的应答。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Smad3 抗体 (38-Q) 参考文献:

  1. 通过 PCR 克隆方法揭示 Rab 和 Rho GTP 结合蛋白亚家族的复杂性。  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
  2. 对活体小鼠中 Smad2/3 依赖性 TGF-beta 信号传导的全面分析表明，组织对损伤有明显的特异性反应。  |  Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
  3. Smads 和染色质调节  |  van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
  4. 通过 Smad 途径传递 TGF-beta 信号。  |  Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
  5. Smad3在磷酸盐诱导的血管平滑肌细胞钙化中起抑制作用。  |  Shimokado, A., et al. 2014. Exp Mol Pathol. 97: 458-64. PMID: 25303897
  6. Smad3促进多柔比星治疗心脏的不良心血管重塑和功能障碍。  |  Cobb, MS., et al. 2022. Am J Physiol Heart Circ Physiol. 323: H1091-H1107. PMID: 36269647
  7. MADR2 位于 18q21，编码一种受 TGFbeta 调节的 MAD 相关蛋白，在结直肠癌中发生功能突变。  |  Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
  8. 与受体相关的 Mad 同源物协同作用，成为 TGF-beta 反应的效应因子。  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
  9. DPC4 和 SMAD 蛋白在 TGF-beta 信号通路中的合作关系。  |  Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
  10. Smad6 可抑制 TGF-beta 超家族的信号传导。  |  Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
  11. Smad8介导ALK-2[校正]受体丝氨酸激酶的信号传导。  |  Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
  12. TGF-beta 信号通过 SMAD 蛋白从细胞膜传递到细胞核。  |  Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997

订购信息

产品名称产品编号规格价格数量收藏夹

Smad3 抗体 (38-Q)

sc-101154
50 µg/0.5 ml
$333.00

Smad3 (38-Q): m-IgGκ BP-HRP 套装

sc-534535
50 µg Ab; 40 µg BP
$354.00

Smad3 (38-Q): m-IgG2a BP-HRP 套装

sc-546192
50 µg Ab; 10 µg BP
$354.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 5 out of 5 by from works well for WBWorks well for neonatal mouse lungs at the concentration of 30ug of protein. Ab dilution will be 1:250 for overnight at 4oC
Date published: 2024-12-13
Rated 5 out of 5 by from good WB outcomespecific and clear bands on C2C12 cells using this antibody
Date published: 2024-06-20
Rated 5 out of 5 by from my review for Smad3 (38-Q, sc-101154) antibodyI tested a free antibody for Smad3 (38-Q, sc-101154) antibody using mouse fibrotic kidney samples for Western blot. We loaded 80 ug proteins in each slot in the SDS gel. The antibody was diluted in 1:500 in 3% BSA/PBST and incubated overnight with the blot. We can observe a very clear band around 55 kd. We have repeated this experiment several times and used the fresh diluted antibody on the fresh blot.
Date published: 2018-05-26
Rated 5 out of 5 by from 抗体很好之前用的好多家都不好，因为样品浓度低，这个抗体第一次就出条带了，只能说抗体特异性好，之前都打不开网页，所以到现在才评论。
Date published: 2018-04-28
Rated 4 out of 5 by from Good for WB1:100 diluted for wb of rat protein ,the band is clear and special
Date published: 2018-01-24
Rated 5 out of 5 by from works really wellThis antibody has been tested on the Infrared detection system. It works really well and I strongly recommend
Date published: 2017-02-06
Rated 5 out of 5 by from Well publishedSmad3 (38-Q) monoclonal antibody is well published for Western blot. Highly recommended!
Date published: 2016-12-20
Rated 5 out of 5 by from good results in WBI've obtained good results in WB, setting the dilution to 1:100.
Date published: 2016-11-24
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Smad3 抗体 (38-Q) is rated 4.8 out of 5 by 13.
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