Date published: 2025-9-6

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Smad1/2/3 抗体 (H-2): sc-7960

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说明书
  • Smad1/2/3 抗体 H-2 是小鼠单克隆 IgG2a κ,Smad1/2/3抗体, 在78篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-465的抗原,代表全长Smad1(human起源
  • Smad1/2/3 抗体 (H-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat, human 和mink 来源的 Smad1, Smad2, Smad3
  • 抗Smad1/2/3抗体(H-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-7960 X, 200 µg/0.1 ml)
  • 关于如何获取Smad1/2/3 (H-2): sc-7960免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 Smad1/2/3 抗体 (H-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Smad1/2/3 抗体 (H-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Smad1/2/3抗体(H-2)是一种小鼠单克隆IgG2a kappa轻链抗体,用于检测多种物种(包括小鼠、大鼠、人类和水貂)的Smad1、Smad2和Smad3蛋白。Smad1/2/3小鼠单克隆抗体(H-2)靶向全长人类Smad1蛋白的氨基酸1-465,确保全面识别这些关键的信号分子。Smad蛋白在果蝇中也被称为Mothers against dpp(Mad),在转化生长因子-β(TGFβ)和骨形态发生蛋白(BMP)信号通路中作为下游效应因子发挥关键作用。Smad1(Mad1或JV4-1)和Smad5是BMP2和BMP4功能的组成部分,而Smad2(Mad2或JV18-1)和Smad3则是介导TGFβ和激活素诱导的生长调节的关键。这些途径是细胞增殖、分化、凋亡等许多细胞过程的基础,因此Smad蛋白对于维持细胞稳态和发育至关重要。Smad1/2/3单克隆抗体(H-2)用途广泛,在蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学(IHC)和酶联免疫吸附测定(ELISA)中均表现出色。Smad1/2/3抗体(H-2)有助于Smad介导的信号传导研究,支持对各种生理和病理状况的研究,包括癌症、纤维化和发育障碍。了解Smad蛋白的调节机制,例如Smad6和Smad7的抑制作用,有助于更深入地了解TGFβ/BMP途径的调节。Smad1/2/3单克隆抗体(H-2)有非结合型和多种结合型,包括HRP、PE、FITC和Alexa Fluor®变体,可用于研究人员探索复杂的细胞信号传导和基因表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Smad1/2/3 抗体 (H-2)

sc-7960
200 µg/ml
RMB2377.00

Smad1/2/3 (H-2): m-IgG Fc BP-HRP 套装

sc-525443
200 µg Ab; 10 µg BP
RMB2662.00

Smad1/2/3 (H-2): m-IgG2a BP-HRP 套装

sc-546258
200 µg Ab; 10 µg BP
RMB2662.00

Smad1/2/3 抗体 (H-2) X

sc-7960 X
200 µg/0.1 ml
RMB2377.00

Smad1/2/3 抗体 (H-2) AC

sc-7960 AC
500 µg/ml, 25% agarose
RMB3129.00

Smad1/2/3 抗体 (H-2) HRP

sc-7960 HRP
200 µg/ml
RMB2377.00

Smad1/2/3 抗体 (H-2) FITC

sc-7960 FITC
200 µg/ml
RMB2482.00

Smad1/2/3 抗体 (H-2) PE

sc-7960 PE
200 µg/ml
RMB2580.00

Smad1/2/3 抗体 (H-2) Alexa Fluor® 488

sc-7960 AF488
200 µg/ml
RMB2685.00

Smad1/2/3 抗体 (H-2) Alexa Fluor® 546

sc-7960 AF546
200 µg/ml
RMB2685.00

Smad1/2/3 抗体 (H-2) Alexa Fluor® 594

sc-7960 AF594
200 µg/ml
RMB2685.00

Smad1/2/3 抗体 (H-2) Alexa Fluor® 647

sc-7960 AF647
200 µg/ml
RMB2685.00

Smad1/2/3 抗体 (H-2) Alexa Fluor® 680

sc-7960 AF680
200 µg/ml
RMB2685.00

Smad1/2/3 抗体 (H-2) Alexa Fluor® 790

sc-7960 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-7960 X be used instead of the standard form sc-7960?

Asked by: cjMara
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from greati use this antibody with 1:1000 dilution in MEF cells. It works very well.
Date published: 2018-01-02
Rated 5 out of 5 by from very goodThe results obtained by Western blot analysis were very good, on the process MV 1 expression (A) and (B) NIH/3T3 cell lysate.
Date published: 2017-01-15
Rated 5 out of 5 by from The antibody is very good to combine withThe antibody is very good to combine with antigen and the background is clear
Date published: 2015-10-26
Rated 5 out of 5 by from Good Western blot analysis of Smad2/3 phosphorylationGood Western blot analysis of Smad2/3 phosphorylation in untreated and TGF -treated Mv 1 Lu whole cell lysates. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Good nuclear and cytoplasmic staining withGood nuclear and cytoplasmic staining with paraffin-embedded human esophagus samples. -SCBT QC
Date published: 2015-02-10
Rated 5 out of 5 by from Strong band at correct mw Mv 1 Lu and NIH/3T3Strong band at correct mw Mv 1 Lu and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2013-11-05
Rated 5 out of 5 by from Very good for WB and IPVery good for WB and IP.
Date published: 2009-04-15
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Smad1/2/3 抗体 (H-2) is rated 5.0 out of 5 by 7.
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