Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
SLUG Antibody (A-7) - Western Blotting - Image 355450
SLUG 抗体 (A-7): sc-166476. HeLa (A), PC-3 (B), MDA-MB-231 (C) 和 Hep G2 (D) 全细胞裂解液及小鼠胎盘组织提取物 (E) 中 SLUG 表达的 Western 印迹分析.
SLUG 抗体 (A-7): sc-166476. HeLa (A), PC-3 (B), MDA-MB-231 (C) 和 Hep G2 (D) 全细胞裂解液及小鼠胎盘组织提取物 (E) 中 SLUG 表达的 Western 印迹分析.

SLUG 抗体 (A-7): sc-166476

3.8(31)
寫評論提問

说明书
  • SLUG 抗体 A-7 是小鼠单克隆 IgG1 κ，SLUG抗体, 在173篇文献中引用，规格为200 µg/ml
  • 免疫human物种的SLUG的氨基酸21-160
  • SLUG 抗体 (A-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SLUG
  • 抗SLUG抗体（A-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-166476 X, 200 µg/0.1 ml)
  • 关于如何获取SLUG (A-7): sc-166476的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 SLUG 抗体 (A-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SLUG 抗体 (A-7) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

SLUG抗体（A-7）是一种IgG1 κ小鼠单克隆SLUG抗体（也称为SNAI2抗体、snail家族转录抑制因子2抗体或SNAIL2抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的SLUG蛋白。SLUG抗体（A-7）既可以是非偶联的抗SLUG抗体形式，也可以是多种偶联形式的抗SLUG抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SNAIL发育调控蛋白家族是一组广泛保守的锌指蛋白，它们调控转录并包括哺乳动物蛋白SLUG、SNAI 1（果蝇SNAIL的人类同源物）和Smuc。SNAI 1和SLUG在胎盘以及成人心脏、肝脏和骨骼肌中表达。SNAI 1和相应的小鼠同源物Sna均包含三个经典锌指和一个非典型锌指，而SLUG包含五个锌指区域和位于氨基末端的转录抑制结构域，这使得SLUG能够作为基因表达的负调节因子。SLUG涉及人类胚胎中神经嵴细胞的产生和迁移，并有助于肢芽的发育。此外，SLUG还构成了一种细胞抗凋亡转录因子，可有效防止剥夺IL-3的小鼠pro-B细胞凋亡。来自小鼠骨骼肌细胞的SNAIL相关基因Smuc在骨骼肌和胸腺中高表达，并且同样可以抑制基因转录。Smuc优先与DNA上的CAGGTG和CACCTG E-box基序（CANNTG）结合，并涉及Smuc羧基末端区域的五个假定DNA结合锌指结构域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SLUG 抗体 (A-7) 参考文献:

  1. 人类转录因子 SLUG：神经管畸形患者的突变分析和 Slug 亚家族定义区域的错义突变 (D119E) 鉴定。  |  Stegmann, K., et al. 1999. Mutat Res. 406: 63-9. PMID: 10479723
  2. SLUG是一种具有抗细胞凋亡活性的ces-1相关锌指转录因子基因，是E2A-HLF肿瘤蛋白的下游靶标。  |  Inukai, T., et al. 1999. Mol Cell. 4: 343-52. PMID: 10518215
  3. 人类蜗牛（SNAI1）基因的特征及作为颅骨发育不良症主要疾病基因的排除。  |  Twigg, SR. and Wilkie, AO. 1999. Hum Genet. 105: 320-6. PMID: 10543399
  4. 人类 SNAIL 基因（SNAI1）及相关加工假基因（SNAI1P）的基因组结构, 表达和染色体位置。  |  Paznekas, WA., et al. 1999. Genomics. 62: 42-9. PMID: 10585766
  5. 一种新型蜗牛相关转录因子 Smuc 通过特定的 E-box 底座调节碱性螺旋-环-螺旋转录因子的活性。  |  Kataoka, H., et al. 2000. Nucleic Acids Res. 28: 626-33. PMID: 10606664
  6. 果蝇蜗牛基因鼠同源物 Sna 的克隆和发育表达。  |  Nieto, MA., et al. 1992. Development. 116: 227-37. PMID: 1483390
  7. USP5通过稳定肝细胞癌中的SLUG来促进上皮-间质转化。  |  Meng, J., et al. 2019. Theranostics. 9: 573-587. PMID: 30809294
  8. 谷氨酰胺耗竭调节Slug以促进胰腺癌的EMT和转移。  |  Recouvreux, MV., et al. 2020. J Exp Med. 217: PMID: 32510550
  9. NELF-E-SLUG-KAT2B表观遗传轴在乳腺癌发生中的依赖性。  |  Zhang, J., et al. 2023. Nat Commun. 14: 2439. PMID: 37117180
  10. TRIB3通过与MDM2的物理相互作用抑制SLUG降解，从而促进肺纤维化。  |  Lv, X., et al. 2023. Acta Pharm Sin B. 13: 1631-1647. PMID: 37139431
  11. 人类 SLUG 基因的组织, 表达和在 8q 上的染色体图谱位置。  |  Cohen, ME., et al. 1998. Genomics. 51: 468-71. PMID: 9721220
  12. 小鼠 Slug（Slugh）基因的基因组结构, 表达和染色体定位。  |  Jiang, R., et al. 1998. Biochim Biophys Acta. 1443: 251-4. PMID: 9838149

订购信息

产品名称产品编号规格价格数量收藏夹

SLUG 抗体 (A-7)

sc-166476
200 µg/ml
RMB2377.00

SLUG (A-7): m-IgG Fc BP-HRP 套装

sc-528976
200 µg Ab; 10 µg BP
RMB2662.00

SLUG (A-7): m-IgGκ BP-HRP 套装

sc-521624
200 µg Ab, 40 µg BP
RMB2662.00

SLUG (A-7): m-IgG1 BP-HRP 套装

sc-543249
200 µg Ab; 20 µg BP
RMB2662.00

SLUG 抗体 (A-7) X

sc-166476 X
200 µg/0.1 ml
RMB2377.00

SLUG 抗体 (A-7) AC

sc-166476 AC
500 µg/ml, 25% agarose
RMB3129.00

SLUG 抗体 (A-7) HRP

sc-166476 HRP
200 µg/ml
RMB2377.00

SLUG 抗体 (A-7) FITC

sc-166476 FITC
200 µg/ml
RMB2482.00

SLUG 抗体 (A-7) PE

sc-166476 PE
200 µg/ml
RMB2580.00

SLUG 抗体 (A-7) Alexa Fluor® 488

sc-166476 AF488
200 µg/ml
RMB2685.00

SLUG 抗体 (A-7) Alexa Fluor® 546

sc-166476 AF546
200 µg/ml
RMB2685.00

SLUG 抗体 (A-7) Alexa Fluor® 594

sc-166476 AF594
200 µg/ml
RMB2685.00

SLUG 抗体 (A-7) Alexa Fluor® 647

sc-166476 AF647
200 µg/ml
RMB2685.00

SLUG 抗体 (A-7) Alexa Fluor® 680

sc-166476 AF680
200 µg/ml
RMB2685.00

SLUG 抗体 (A-7) Alexa Fluor® 790

sc-166476 AF790
200 µg/ml
RMB2685.00

Could you send the protocol of IHC for using this antibody? I can never get the positive result when using this antibody to do IHC in my protocol

Asked by: zhuyyyyy
Thank you for your question. The specific IHC protocol for this antibody is not available, but please refer to the general IHC protocol by visiting this link: https://www.scbt.com/zh/resources/protocols/immunoperoxidase-staining Please contact our Asian Technical Service team if you have additional questions, we are available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 12
Date published: 2021-09-26

Can you please tell about the antigen retrieval solution to be used for IHC using this antibody?

Asked by: Neeti Swarup
Thank you for your question. We recommend heat treatment with citrate buffer. You can find the protocol for antigen retrieval with IHC in our recommended protocol: https://www.scbt.com/scbt/resources/protocols
Answered by: Tech Support Europe
Date published: 2020-08-05

Does this antibody (sc-166476) have cross-reactivity to Snail protein?

Asked by: nobu
Thank you for your question. This mouse monoclonal antibody is specific for SLUG and does not cross-react with the closely related family protein, Snail, also designated SNAI 1.
Answered by: Technical Support
Date published: 2019-01-25

I would like to request a small free sample of SLUG Antibody (A-7): sc-166476. How do I proceed to order? Thank you very much in advance!

Asked by: Ragnhild
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

I am interested in performing a double stain, but how can I do this if I am only using mouse monoclonals?

Asked by: Cweed
To perform a double stain, we recommend using directly conjugated primary antibodies. This product, sc-166476, is available conjugated to AF488, AF647, and FITC.
Answered by: TechService7
Date published: 2016-12-21

Conventional secondary antibodies are no longer available, what can I use as my detection reagent?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-21

Will this cross react with mouse?

Asked by: Cathy193
Thank you for your question. sc-166476 does not cross react with mouse samples. However, we do have another mouse monoclonal antibody against SLUG that does react with mouse, sc-166902: https://www.scbt.com/scbt/product/slug-antibody-c-7.
Answered by: Tech Service
Date published: 2016-09-26
  • y_2025, m_8, d_31, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_7
  • loc_zh_CN, sid_166476, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 110ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from No bands in Western blottingIt is disappointing that no bands were visible in EMT-induced cells.
Date published: 2024-08-18
Rated 4 out of 5 by from This antibody works well for western blot.This antibody works well for western blot. I'd recommend it.
Date published: 2024-03-19
Rated 5 out of 5 by from Good antibody for Western blotIt works great - I'd recommend this SLUG antibody.
Date published: 2022-05-05
Rated 1 out of 5 by from No specific bandsWe used the mouse monoclonal SLUG Antibody (A-7) for Western Blot at a dilution 1:500 in milk and its expression was detected in MDA-MB-231 cell line as suggest by data sheet. Nevertheless, we got high background with not-specific bands and low signal at the expected size.
Date published: 2021-10-22
Rated 5 out of 5 by from sc-166476This antibody works very good for western blotting.
Date published: 2021-02-25
Rated 2 out of 5 by from まぁまぁウエスタンブロットで検出可能でした。目的タンパク質以外のバンドも検出されてしまいましたが、なんとか使えました。
Date published: 2020-08-06
Rated 4 out of 5 by from Best antibody we've found for slugTypically westerns for the EMT transcription factors are very dim - this is one of the better antibodies for Slug and is the only one we've found to reliably work.
Date published: 2019-05-06
Rated 4 out of 5 by from Great productIt is good antibodies in WB. It works in dilution 1:1000.
Date published: 2018-07-14
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_31
  • loc_zh_CN, sid_166476, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
SLUG 抗体 (A-7) is rated 3.8 out of 5 by 31.
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_31
  • loc_zh_CN, sid_166476, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 134ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。