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Shh Antibody (G-5) - Western Blotting - Image 147815
Shh 抗体 (G-5): sc-373779. 非转染: sc-117752 (A) 和小鼠 Shh 转染: sc-123543 (B) 293T 全细胞裂解液中 Shh 表达的 Western 印迹分析.
Shh Antibody (G-5) - Western Blotting - Image 147815
Shh 抗体 (G-5): sc-373779. 非转染: sc-117752 (A) 和小鼠 Shh 转染: sc-123543 (B) 293T 全细胞裂解液中 Shh 表达的 Western 印迹分析.
Shh 抗体 (G-5): sc-373779. 非转染: sc-117752 (A) 和小鼠 Shh 转染: sc-123543 (B) 293T 全细胞裂解液中 Shh 表达的 Western 印迹分析.

Shh 抗体 (G-5): sc-373779

5.0(1)
寫評論提問
说明书
  • Shh抗体(G-5)是小鼠单克隆IgG1 κ, 在7篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Shh的氨基酸41-200
  • 推荐用于 mouse, rat 和 human 来源的a broad range of hedgehog family proteins WB, IP, IF 和 ELISA检测; 也和以下物种反应，包括： 和 equine and canine
  • 看 Shh (E-1): sc-365112，可查看有标记的 Shh 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是Shh Antibody (G-5) for WB applications. 的首选辅助检测试剂，这些试剂现与Shh Antibody (G-5) 打包提供（请参阅下面的订购信息）。
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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

果蝇段极性基因hedgehog（hh）编码一种前体蛋白，该蛋白通过自切割产生氨基端和羧基端肽。这两种蛋白质都被分泌出来，并在胚胎和成虫盘图案形成中发挥作用。已经鉴定出几种果蝇hh的脊椎动物同源物，包括Sonic hedgehog（Shh）（也称为Vhh-1）、Desert hedgehog（Dhh）和Indian hedgehog（Ihh）。它们都含有氨基端信号肽，并显然作为分泌蛋白发挥作用，参与介导各种细胞-细胞相互作用。Shh与果蝇hh相似，因为它被加工以产生保留在细胞表面或附近的氨基端分泌肽和羧基端糖基化更易扩散的肽。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Shh 抗体 (G-5) 参考文献:

  1. 声波刺猬（SHH）通路与胃癌中癌干细胞基因的相互作用。  |  Samadani, AA. and Akhavan-Niaki, H. 2015. Med Oncol. 32: 48. PMID: 25636508
  2. 声波刺猬（Shh）信号在膀胱癌干细胞和肿瘤发生中的作用。  |  Syed, IS., et al. 2016. Curr Urol Rep. 17: 11. PMID: 26757905
  3. 音速刺猬信号与海马神经可塑性  |  Yao, PJ., et al. 2016. Trends Neurosci. 39: 840-850. PMID: 27865563
  4. 靶向神经疾病中的Sonic Hedgehog信号传导。  |  Patel, SS., et al. 2017. Neurosci Biobehav Rev. 74: 76-97. PMID: 28088536
  5. 四肢发育中的Sonic Hedgehog信号传导  |  Tickle, C. and Towers, M. 2017. Front Cell Dev Biol. 5: 14. PMID: 28293554
  6. 哮喘中的Sonic hedgehog (Shh)和CC趋化因子配体2信号通路。  |  Wang, XZ., et al. 2019. J Chin Med Assoc. 82: 343-350. PMID: 31058710
  7. 星形胶质细胞中的声波刺猬信号传导。  |  Hill, SA., et al. 2021. Cell Mol Life Sci. 78: 1393-1403. PMID: 33079226
  8. SHH髓母细胞瘤异质性的发育基础。  |  Gold, MP., et al. 2024. Nat Commun. 15: 270. PMID: 38191555
  9. 正确进行靶向治疗：直接抑制Sonic Hedgehog治疗Sonic Hedgehog髓母细胞瘤。  |  Nellan, A. and Jackson, S. 2024. Neuro Oncol. 26: 623-624. PMID: 38290484
  10. 调节Sonic Hedgehog（SHH）通路以创建快速CNS-TB模型：促进药物发现。  |  Mubarak, MM., et al. 2024. J Neuroimmunol. 397: 578471. PMID: 39536643

订购信息

产品名称产品编号规格价格数量收藏夹

Shh 抗体 (G-5)

sc-373779
200 µg/ml
CNY2422.00

Shh (G-5): m-IgG Fc BP-HRP 套装

sc-540399
200 µg Ab; 10 µg BP
CNY2715.00

Shh (G-5): m-IgG1 BP-HRP 套装

sc-542095
200 µg Ab; 20 µg BP
CNY2715.00

Hi, can we use sc-373779 for IF and WB to detect SHH in rat hippocampus (using either brain slices or brain tissue)? If yes, are there any published papers on this using this antibody? Thanks!

Asked by: Jenn12
Thank you for your question. sc-373779 is recommended for mouse , rat and human. Here is a publication for western blot in rat samples . https://pubmed.ncbi.nlm.nih.gov/30344694/
Answered by: BlakeJ
Date published: 2023-03-14

Is there using sc-373779:Shh(G-5) antibody in mouse skin sample?

Asked by: qqdada1234
Thank you for your question. This antibody is recommended for detection of a broad range of hedgehog family proteins in mouse samples by Western blotting. We do not have any data or references specifically in skin tissue.
Answered by: Technical Support
Date published: 2019-05-25

Are there any issues with using sc-373779: Shh (G-5) mouse monoclonal primary antibody on mouse samples?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-373779 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by JM from Nice Western blot data of Shh expression in nonNice Western blot data of Shh expression in non-transfected and mouse Shh transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-11-18
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Shh 抗体 (G-5) is rated 5.0 out of 5 by 1.
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