Date published: 2025-9-9
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Shh Antibody (E-1) - Western Blotting - Image 137799
Shh 抗体 (E-1): sc-365112. IMR-32 全细胞裂解液 (A) 和大鼠脑组织提取物 (B) 中 Shh 表达的 Western 印迹分析.
Shh 抗体 (E-1): sc-365112. IMR-32 全细胞裂解液 (A) 和大鼠脑组织提取物 (B) 中 Shh 表达的 Western 印迹分析.

Shh 抗体 (E-1): sc-365112

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说明书
  • Shh 抗体 E-1 是小鼠单克隆 IgG1 κ，Shh抗体, 在53篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Shh的N-terminus附近的氨基酸25-57之间
  • Shh 抗体 (E-1) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 avian来源的Ihh and Shh; 也和以下物种反应，包括： 和 equine, canine, porcine and avian
  • 抗Shh抗体（E-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Shh (E-1): sc-365112的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Shh Antibody (E-1) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Shh Antibody (E-1) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Shh抗体（E-1）是一种IgG1 κ型鼠单克隆Shh抗体（也称为sonic hedgehog抗体），它通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类来源的Shh蛋白。Shh抗体（E-1）既可以是非偶联的抗Shh抗体形式，也可以是多种偶联形式的抗Shh抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。果蝇的段极性基因hedgehog (hh) 编码一种前体蛋白，该蛋白经过自切割产生氨基端和羧基端肽。这两种蛋白质都被分泌出来，似乎在胚胎和成虫盘图案形成中发挥作用。已经鉴定出几种果蝇hh的脊椎动物同源物。这些包括Sonic hedgehog (Shh)（也称为Vhh-1）、Desert hedgehog (Dhh) 和Indian hedgehog (Ihh)。它们都含有氨基端信号肽，并显然作为分泌蛋白发挥作用，参与介导各种细胞-细胞相互作用。Shh与果蝇hh相似，因为它被加工以产生保留在细胞表面或附近的氨基端分泌肽和羧基端糖基化更易扩散的肽。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Shh 抗体 (E-1) 参考文献:

  1. 音速刺猬信号与牙齿发育  |  Hosoya, A., et al. 2020. Int J Mol Sci. 21: PMID: 32111038
  2. Sonic Hedgehog髓母细胞瘤中的种系延长因子突变。  |  Waszak, SM., et al. 2020. Nature. 580: 396-401. PMID: 32296180
  3. METTL3在Sonic hedgehog信号传导中调节PTCH1和GLI2的m6A甲基化，促进SHH髓母细胞瘤的肿瘤进展。  |  Zhang, ZW., et al. 2022. Cell Rep. 41: 111530. PMID: 36288719
  4. Sonic hedgehog信号在颅面发育中的作用。  |  Xu, J., et al. 2023. Differentiation. 133: 60-76. PMID: 37481904
  5. 刺猬信号的长与短  |  Johnson, RL. and Tabin, C. 1995. Cell. 81: 313-6. PMID: 7736584
  6. 不同浓度的声刺猬自体蛋白水解氨基末端裂解产物对底板和运动神经元的诱导作用  |  Roelink, H., et al. 1995. Cell. 81: 445-55. PMID: 7736596
  7. 音速刺猬的长程硬核诱导：氨基末端裂解产物的直接作用和环磷酸腺苷信号通路的调节。  |  Fan, CM., et al. 1995. Cell. 81: 457-65. PMID: 7736597
  8. 中枢神经系统外植体中不同腹侧细胞类型的诱导需要19K形式的Sonic hedgehog。  |  Martí, E., et al. 1995. Nature. 375: 322-5. PMID: 7753196
  9. 音速刺猬诱导腹侧前脑神经元分化：神经管腹侧模式化的共同信号  |  Ericson, J., et al. 1995. Cell. 81: 747-56. PMID: 7774016
  10. 蛋白激酶 A 在刺猬信号转导和果蝇胚盘发育中的功能  |  Li, W., et al. 1995. Cell. 80: 553-62. PMID: 7867063
  11. 音速刺猬（Sonic hedgehog）是一种假定的信号分子家族成员，与中枢神经系统极性的调节有关。  |  Echelard, Y., et al. 1993. Cell. 75: 1417-30. PMID: 7916661
  12. Sonic hedgehog介导ZPA的极化活性。  |  Riddle, RD., et al. 1993. Cell. 75: 1401-16. PMID: 8269518

订购信息

产品名称产品编号规格价格数量收藏夹

Shh 抗体 (E-1)

sc-365112
200 µg/ml
RMB2377.00

Shh (E-1): m-IgG Fc BP-HRP 套装

sc-552003
200 µg Ab; 10 µg BP
RMB2662.00

Shh 抗体 (E-1) AC

sc-365112 AC
500 µg/ml, 25% agarose
RMB3129.00

Shh 抗体 (E-1) HRP

sc-365112 HRP
200 µg/ml
RMB2377.00

Shh 抗体 (E-1) FITC

sc-365112 FITC
200 µg/ml
RMB2482.00

Shh 抗体 (E-1) PE

sc-365112 PE
200 µg/ml
RMB2580.00

Shh 抗体 (E-1) Alexa Fluor® 488

sc-365112 AF488
200 µg/ml
RMB2685.00

Shh 抗体 (E-1) Alexa Fluor® 546

sc-365112 AF546
200 µg/ml
RMB2685.00

Shh 抗体 (E-1) Alexa Fluor® 594

sc-365112 AF594
200 µg/ml
RMB2685.00

Shh 抗体 (E-1) Alexa Fluor® 647

sc-365112 AF647
200 µg/ml
RMB2685.00

Shh 抗体 (E-1) Alexa Fluor® 680

sc-365112 AF680
200 µg/ml
RMB2685.00

Shh 抗体 (E-1) Alexa Fluor® 790

sc-365112 AF790
200 µg/ml
RMB2685.00

Shh (E-1) 中和勝肽

sc-365112 P
100 µg/0.5 ml
RMB511.00

Hi, Does it detect N-terminal or C-terminal of the Shh?

Asked by: Atefeh
Thank you for your question. sc-365112 Shh (E-1) detects the N-terminal.
Answered by: BlakeJ
Date published: 2025-04-04

Hey, Can we use sc-365112 for IF and WB to detect SHH in rat hippocampus (using either brain slices or brain tissue)? If yes, are there any published papers on this using this antibody? Thanks!

Asked by: Jenn12
Thank you for your question. sc-365112 is recommended for mouse , rat and human. Here are publications for rat in western blot . https://pubmed.ncbi.nlm.nih.gov/27644702/ https://pubmed.ncbi.nlm.nih.gov/29244790/ https://pubmed.ncbi.nlm.nih.gov/29721855/ We do not have any publications for rat hippocampus.
Answered by: BlakeJ
Date published: 2023-02-15

Hi, I would like to perform RNA-binding protein immunoprecipitation experiment and am looking for anti-SHH (human). Will Shh Antibody (E-1): sc-365112 be suitable for this experiment? Thank you!

Asked by: IC9527
Thank you for your question. This antibody is recommended and warrantied for immunoprecipitation (IP) of the target protein based on the performance of the antibody in other applications. However, we do not have any data or product citations using this antibody for IP at this time. Please check back for updates!
Answered by: Technical Support
Date published: 2020-05-04

I would like to use it in intervertebral tissue of adult zebrafish, is it possible?

Asked by: jackie2819
Thank you for your question. This antibody has not yet been tested in zebrafish and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-09-30

I would like to use Shh Antibody (E-1): sc-365112 for western blot with mouse skin. Is this possible?

Asked by: qqdada1234
Thank you for your question. This antibody is recommended for detection of Ihh and Shh in mouse samples by Western blotting. We do not have any data or references specifically in skin tissue.
Answered by: Technical Support
Date published: 2019-05-25

I would like to use one of your Shh antibodies for labelling ballooned hepatocytes in mouse

Asked by: Archi
Thank you for your question. This antibody, sc-365112, is a great choice for labeling mouse samples. Please contact Technical Service if you have any further questions.
Answered by: Tech Service
Date published: 2019-05-10

I would like to use Shh Antibody (E-1): sc-365112 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-365112 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-02-01
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Rated 4 out of 5 by from 不错呢hh 比较容易跑出来，效果还蛮好的,但是WB跑出来的条带中并不是单克隆抗体呢抗体效价很高，条带比较清晰
Date published: 2018-02-28
Rated 4 out of 5 by from It works!抗体效价满足要求，特异性方面也满足要求，可以作为比较好的选择进行下一步的实验，我会继续选择这个货号的。
Date published: 2017-09-21
Rated 3 out of 5 by from western blot3T3-L1 cell lysis has about 23Kd strong band , have some non specific band, work well.
Date published: 2017-08-14
Rated 5 out of 5 by from ExcellentI received this a month ago, I am very happy to use this. I work excellently,
Date published: 2017-06-21
Rated 4 out of 5 by from Buen ResultadoProbamos este producto en IHQ e IF de tejido de Hipófisis y funciona.
Date published: 2017-04-11
Rated 4 out of 5 by from Good for western blotWorked well (1:200) with western blotting on human adrenal tissue
Date published: 2017-02-13
Rated 3 out of 5 by from Fonctionne en WB sourisEn WB :Une bande à 45kDa comme attendu. en immunohistochimie sur des surrénales de souris: pas de marquage
Date published: 2017-02-02
Rated 5 out of 5 by from Very Strong IF ImageWe used this antibody for IF staining He/la cells and got a very intense signal when looking at cytoplasmic regions
Date published: 2016-12-28
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Shh 抗体 (E-1) is rated 4.1 out of 5 by 14.
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