Date published: 2025-9-6
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SH-PTP1 Antibody (D-11) - Western Blotting - Image 398973
SH-PTP1 抗体 (D-11): sc-7289. HEL 92. 1. 7 (A), TF-1 (B), CCRF-CEM (C), HL-60 (D), U-937 (E) 和 Raji (F) 全细胞裂解液中 SH-PTP1 表达的 Western 印迹分析. 所用检测试剂: m-IgG3 BP-HRP: sc-533670.
SH-PTP1 抗体 (D-11): sc-7289. HEL 92. 1. 7 (A), TF-1 (B), CCRF-CEM (C), HL-60 (D), U-937 (E) 和 Raji (F) 全细胞裂解液中 SH-PTP1 表达的 Western 印迹分析. 所用检测试剂: m-IgG3 BP-HRP: sc-533670.

SH-PTP1 抗体 (D-11): sc-7289

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寫評論提問

说明书
  • SH-PTP1 抗体 D-11 是小鼠单克隆 IgG3 κ，SH-PTP1抗体, 在60篇文献中引用，规格为200 µg/ml
  • 特定用于在C-terminus的SH-PTP1的human起源的氨基酸576-595之间的表位图谱（与相应的鼠序列仅有一个氨基酸不同）
  • 推荐用于 mouse, rat 和 human 来源的SH-PTP1 WB, IP, IF, IHC(P), FCM 和 ELISA检测; 也和以下物种反应，包括： 和 canine and porcine
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取SH-PTP1 (D-11): sc-7289的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP 是SH-PTP1 抗体 (D-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与SH-PTP1 抗体 (D-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 SH-PTP 抗体 包括 SH-PTP2

SH-PTP1抗体（D-11）是一种小鼠单克隆IgG3 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的SH-PTP1。SH-PTP1抗体（D-11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。SH-PTP1，也称为非受体型蛋白酪氨酸磷酸酶6，通过去磷酸化靶蛋白上的酪氨酸残基来调节细胞信号通路，从而在维持细胞中酪氨酸磷酸化平衡方面发挥关键作用。这种调节在造血细胞中尤为重要，SH-PTP1可调节细胞生长、分化及免疫反应中各种信号通路的活性。SH-PTP1中两个SH2结构域的存在使其能够与自磷酸化受体上特定的含磷酪氨酸位点相互作用，从而影响对细胞功能至关重要的下游信号传导事件。了解SH-PTP1在这些过程中的作用有助于深入了解其在癌症和自身免疫性疾病等疾病中的潜在影响，使SH-PTP1 (D-11)抗体成为研究人员研究这些疾病的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SH-PTP1 抗体 (D-11) 参考文献:

  1. 鉴定人类含 src 同源物 2 的蛋白-酪氨酸-磷酸酶：果蝇开瓶器的推定同源物。  |  Freeman, RM., et al. 1992. Proc Natl Acad Sci U S A. 89: 11239-43. PMID: 1280823
  2. 造血细胞内蛋白酪氨酸磷酸酶的特征：描述了一种含有 SH2 结构域和另一种富含脯氨酸, 谷氨酸, 丝氨酸和苏氨酸序列的磷酸酶。  |  Matthews, RJ., et al. 1992. Mol Cell Biol. 12: 2396-405. PMID: 1373816
  3. 一种蛋白酪氨酸磷酸酶，其序列与蛋白酪氨酸激酶的 SH2 结构域相似。  |  Shen, SH., et al. 1991. Nature. 352: 736-9. PMID: 1652101
  4. 含 SH2 结构域的蛋白酪氨酸磷酸酶：特性, 在造血细胞中的优先表达以及在人类染色体 12p12-p13 上的定位。  |  Yi, TL., et al. 1992. Mol Cell Biol. 12: 836-46. PMID: 1732748
  5. 含 src 同源物 2 的酪氨酸磷酸酶的分离。  |  Plutzky, J., et al. 1992. Proc Natl Acad Sci U S A. 89: 1123-7. PMID: 1736296
  6. 克隆人类主要蛋白酪氨酸磷酸酶的 cDNA。  |  Chernoff, J., et al. 1990. Proc Natl Acad Sci U S A. 87: 2735-9. PMID: 2157211
  7. 丙戊酸通过内皮细胞和老鼠中的SH-PTP1-CDK5-eNOS-Ser(116)信号级联增加一氧化氮的产生。  |  Cho, DH., et al. 2014. Free Radic Biol Med. 76: 96-106. PMID: 25150199
  8. 蛋白酪氨酸磷酸酶 SH-PTP1 的分子内调控：Src 同源 2 结构域的新功能  |  Pei, D., et al. 1994. Biochemistry. 33: 15483-93. PMID: 7528537
  9. 通过酪氨酸磷酸化激活磷酸酪氨酸磷酸酶。  |  Vogel, W., et al. 1993. Science. 259: 1611-4. PMID: 7681217
  10. 含 SH2 的磷酸酪氨酸磷酸酶是蛋白酪氨酸激酶的靶标。  |  Feng, GS., et al. 1993. Science. 259: 1607-11. PMID: 8096088
  11. 小鼠T细胞中磷酸化酪氨酸磷酸酶SH-PTP1的Lck依赖性酪氨酸磷酸化。  |  Lorenz, U., et al. 1994. Mol Cell Biol. 14: 1824-34. PMID: 8114715
  12. 蛋白酪氨酸磷酸酶 SH-PTP1 中两个 Src 同源 2 结构域的不同功能  |  Pei, D., et al. 1996. Proc Natl Acad Sci U S A. 93: 1141-5. PMID: 8577729

订购信息

产品名称产品编号规格价格数量收藏夹

SH-PTP1 抗体 (D-11)

sc-7289
200 µg/ml
RMB2377.00

SH-PTP1 (D-11): m-IgG3 BP-HRP 套装

sc-550542
200 µg Ab; 40 µg BP
RMB2662.00

SH-PTP1 抗体 (D-11) AC

sc-7289 AC
500 µg/ml, 25% agarose
RMB3129.00

SH-PTP1 抗体 (D-11) Alexa Fluor® 488

sc-7289 AF488
200 µg/ml
RMB2685.00

SH-PTP1 抗体 (D-11) Alexa Fluor® 647

sc-7289 AF647
200 µg/ml
RMB2685.00

SH-PTP1 抗体 (D-11) FITC

sc-7289 FITC
200 µg/ml
RMB2482.00

SH-PTP1 抗体 (D-11) HRP

sc-7289 HRP
200 µg/ml
RMB2377.00

SH-PTP1 (D-11) 中和勝肽

sc-7289 P
100 µg/0.5 ml
RMB511.00

SH-PTP1 抗体 (D-11) PE

sc-7289 PE
200 µg/ml
RMB2580.00

We have problems detecting transfected SHP-1 with this Ab, although we detect the protein with an anti-Myc tag Ab. Can you send me the sequence of the epitope that this Ab recognizes, because there are 4 SHP1 isoforms and they differ in the C-terminus.

Asked by: Dimitar
Thank you for your question. This monoclonal antibody, SH-PTP1 (D-11): sc-7289, is specific for an epitope mapping between amino acids 576-595 at the C-terminus of SH-PTP1 of human origin (Uniprot Accession number: P29350).
Answered by: Technical Support
Date published: 2017-09-13

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 1 out of 5 by from Did not work well in HepaRGI tried this out as a sample to test as a housekeeping for HepaRG cells. There was alot of non specific binding and background, I tested it at a 1:5000 dilution.
Date published: 2023-06-28
Rated 4 out of 5 by from Very good!I used this antibody diluted 1:500 in PBS 0,02% Tween for 3 hours at room temperature. It gives a good signal but I also observed a weak aspecific band.
Date published: 2022-01-27
Rated 5 out of 5 by from Good antibody!I used this antibody for detecting SHP1 in some Co-IP samples and it works well in a dilution of 1: 400, quantitative data the sensitivity of this antibody is lower than 0.1 ng. Photo shown here is a WB pic of this antibody probing SHP1 WT and KO Jurkat lysates.
Date published: 2019-04-09
Rated 1 out of 5 by from Works ok for WB, didn't work for IPWB detection was poor in 100 ug protein lysate from primary murine hepatocytes. Ab didn't work for IP.
Date published: 2018-04-30
Rated 5 out of 5 by from Western blotThis antibody had good expression in western blot. (cell line: HUVEC)
Date published: 2017-11-22
Rated 5 out of 5 by from immunoblotA strong band was detected in lysates prepared from human patient samples
Date published: 2017-07-26
Rated 5 out of 5 by from Better than the previous oneThis monoclonal antibody I tested in Jurkat cells and received nice single, strong band.
Date published: 2017-04-17
Rated 5 out of 5 by from Worked well in IHCWorked well in formalin fixed, paraffin-embedded human bone marrow
Date published: 2017-02-21
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SH-PTP1 抗体 (D-11) is rated 4.6 out of 5 by 20.
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