Date published: 2025-9-8
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SGK Antibody (G-4) - Western Blotting - Image 283824
SGK 抗体 (G-4): sc-377360. 在 NIH/3T3 全细胞裂解液中检测 SGK 表达的 Western 印迹分析.
SGK 抗体 (G-4): sc-377360. 在 NIH/3T3 全细胞裂解液中检测 SGK 表达的 Western 印迹分析.

SGK 抗体 (G-4): sc-377360

4.6(7)
寫評論提問

说明书
  • SGK 抗体 G-4 是小鼠单克隆 IgG2a κ，SGK抗体, 在7篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列381-420位于human物种的SGK的C-terminus附近
  • SGK 抗体 (G-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SGK
  • 抗SGK抗体（G-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SGK (G-4): sc-377360的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 SGK 抗体 (G-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与SGK 抗体 (G-4) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 SGK 抗体包括 SGK2, Sgk223, SGK3 和 Sgk269

SGK抗体（G-4）是一种IgG2a κ小鼠单克隆SGK抗体（也被称为SGK1抗体、血清/糖皮质激素调节激酶1抗体或SGK1激酶抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的SGK蛋白。SGK抗体（G-4）既有非偶联形式的抗SGK抗体，也有多种偶联形式的抗SGK抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。血清和糖皮质激素调节激酶（SGK），也被称为SGK1，是一种丝氨酸/苏氨酸蛋白激酶，也是包括蛋白激酶A、G和C的"AGC"亚家族成员之一。SGK在激活某些钾、钠和氯通道方面起着重要作用，这表明它参与了细胞生存、神经元兴奋性和肾钠排泄等过程的调节。SGK包含一个催化结构域，与Akt1（也被称为蛋白激酶B或PKB）最为相似。SGK是PI 3-激酶刺激的生长因子信号的下游靶标，3-磷酸肌醇依赖性蛋白激酶1（PDK1）能够在苏氨酸-256位点磷酸化SGK的激活环。肾上腺皮质类固醇激素醛固酮诱导SGK的转录，通过刺激上皮钠通道活性来介导Na +转运。SGK启动子包含一个糖皮质激素反应元件和一个SP-1调节元件，并且是p53的转录靶标。SGK也是p38 MAPK介导的对高渗应激反应的组成部分。人类SGK基因定位于6q23染色体，编码431个氨基酸的SGK蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

SGK 抗体 (G-4)

sc-377360
200 µg/ml
RMB2377.00

SGK (G-4): m-IgGκ BP-HRP 套装

sc-551106
200 µg Ab; 40 µg BP
RMB2662.00

SGK 抗体 (G-4) AC

sc-377360 AC
500 µg/ml, 25% agarose
RMB3129.00

SGK 抗体 (G-4) HRP

sc-377360 HRP
200 µg/ml
RMB2377.00

SGK 抗体 (G-4) FITC

sc-377360 FITC
200 µg/ml
RMB2482.00

SGK 抗体 (G-4) PE

sc-377360 PE
200 µg/ml
RMB2580.00

SGK 抗体 (G-4) Alexa Fluor® 488

sc-377360 AF488
200 µg/ml
RMB2685.00

SGK 抗体 (G-4) Alexa Fluor® 546

sc-377360 AF546
200 µg/ml
RMB2685.00

SGK 抗体 (G-4) Alexa Fluor® 594

sc-377360 AF594
200 µg/ml
RMB2685.00

SGK 抗体 (G-4) Alexa Fluor® 647

sc-377360 AF647
200 µg/ml
RMB2685.00

SGK 抗体 (G-4) Alexa Fluor® 680

sc-377360 AF680
200 µg/ml
RMB2685.00

SGK 抗体 (G-4) Alexa Fluor® 790

sc-377360 AF790
200 µg/ml
RMB2685.00

Can SGK (G-4): sc-377360 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, SGK (G-4): sc-377360 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Worked very wellA very clear signal was obtained in western blot. the 10% acrylamide gel was adequate to separate the two bands that are obtained.
Date published: 2021-09-19
Rated 5 out of 5 by from Great antibody!1:200 diluted for wb of rat heart protein ,the band is quite strong and special
Date published: 2019-02-25
Rated 4 out of 5 by from It is really goodI purchased this antibody and have good experience. So, I recommend it to you
Date published: 2017-03-10
Rated 5 out of 5 by from Produced publishable WB data on humanThis SGK monoclonal produced published data of WB on human HCT116 cells
Date published: 2017-01-19
Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmicBeautiful immunofluorescence cytoplasmic and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-10
Rated 4 out of 5 by from Clean blot with doublet strong bands at expectedClean blot with doublet strong bands at expected size in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Great antibody for WesternGreat antibody for Western, strong doublet bands detected in mouse kidney tissue extract. -SCBT QC
Date published: 2015-02-18
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SGK 抗体 (G-4) is rated 4.6 out of 5 by 7.
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