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secretin receptor Antibody (E-9) - Western Blotting - Image 66251
胰泌素受体抗体 (E-9): sc-166112. 截短的人重组泌泌素受体融合蛋白的 Western 印迹分析.
secretin receptor Antibody (E-9) - Western Blotting - Image 66251
胰泌素受体抗体 (E-9): sc-166112. 截短的人重组泌泌素受体融合蛋白的 Western 印迹分析.
胰泌素受体抗体 (E-9): sc-166112. 截短的人重组泌泌素受体融合蛋白的 Western 印迹分析.

secretin receptor 抗体 (E-9): sc-166112

3.5(4)
寫評論提問

说明书
  • secretin receptor 抗体 E-9 是小鼠单克隆 IgG1 (kappa轻链)，secretin receptor抗体，规格为200 µg/ml
  • 针对secretin receptor的human起源的N-terminal细胞外域内的氨基酸33-121
  • secretin receptor 抗体 (E-9) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 secretin precursor and active peptide
  • 抗secretin receptor抗体（E-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是secretin receptor Antibody (E-9) for WB applications. 的首选辅助检测试剂，这些试剂现与secretin receptor Antibody (E-9) 打包提供（请参阅下面的订购信息）。
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    描述
    基因信息
    蛋白序列
    说明书与实验方案
    研究信息

    関連項目

    分泌素受体（E-9）抗体是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附试验（ELISA）检测人源分泌素受体蛋白。胰泌素受体（E-9）抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 共轭物。胰泌素受体在调节消化过程中起着至关重要的作用，尤其是在刺激富含碳酸氢盐的胰液分泌方面，它有助于中和十二指肠中的胃酸。这种受体主要存在于胰腺导管细胞和胆管细胞中，胰泌素受体（E-9）单克隆抗体可介导胰泌素的作用，胰泌素是一种由小肠近端 S 细胞产生的 27 氨基酸激素。胰泌素与其受体之间的相互作用可促进碳酸氢盐的分泌，并增强胆管分泌，有助于消化和营养吸收。编码分泌素受体的基因位于人类染色体 11p15.5，其信号通路的变化可能与各种胃肠道疾病有关，这凸显了分泌素在消化系统健康和潜在神经内分泌功能中的重要性。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    secretin receptor 抗体 (E-9) 参考文献:

    1. 人类胰泌素（SCT）：基因结构, 染色体位置和 mRNA 分布。  |  Whitmore, TE., et al. 2000. Cytogenet Cell Genet. 90: 47-52. PMID: 11060443
    2. 胰腺分泌机制  |  Bayliss, WM. and Starling, EH. 1902. J Physiol. 28: 325-53. PMID: 16992627
    3. 胰泌素：前体的结构和 mRNA 的组织分布。  |  Kopin, AS., et al. 1990. Proc Natl Acad Sci U S A. 87: 2299-303. PMID: 2315322
    4. 猪胰泌素的结构。氨基酸序列。  |  Mutt, V., et al. 1970. Eur J Biochem. 15: 513-9. PMID: 5465996
    5. 胰泌素通过增加质膜上的水通道促进大鼠胆管细胞的渗透性水转运。胰泌素诱导水囊蛋白-1水囊转运的证据。  |  Marinelli, RA., et al. 1997. J Biol Chem. 272: 12984-8. PMID: 9148905

    订购信息

    产品名称产品编号规格价格数量收藏夹

    secretin receptor 抗体 (E-9)

    sc-166112
    200 µg/ml
    		CNY2422.00

    secretin receptor (E-9): m-IgG Fc BP-HRP 套装

    sc-527138
    200 µg Ab; 10 µg BP
    		CNY2715.00

    secretin receptor (E-9): m-IgG1 BP-HRP 套装

    sc-532511
    200 µg Ab; 20 µg BP
    		CNY2715.00

    secretin receptor 抗体 (E-9) AC

    sc-166112 AC
    500 µg/ml, 25% agarose
    		CNY3189.00

    secretin receptor 抗体 (E-9) HRP

    sc-166112 HRP
    200 µg/ml
    		CNY2422.00

    secretin receptor 抗体 (E-9) FITC

    sc-166112 FITC
    200 µg/ml
    		CNY2527.00

    secretin receptor 抗体 (E-9) PE

    sc-166112 PE
    200 µg/ml
    		CNY2625.00

    secretin receptor 抗体 (E-9) Alexa Fluor® 488

    sc-166112 AF488
    200 µg/ml
    		CNY2738.00

    secretin receptor 抗体 (E-9) Alexa Fluor® 546

    sc-166112 AF546
    200 µg/ml
    		CNY2738.00

    secretin receptor 抗体 (E-9) Alexa Fluor® 594

    sc-166112 AF594
    200 µg/ml
    		CNY2738.00

    secretin receptor 抗体 (E-9) Alexa Fluor® 647

    sc-166112 AF647
    200 µg/ml
    		CNY2738.00

    secretin receptor 抗体 (E-9) Alexa Fluor® 680

    sc-166112 AF680
    200 µg/ml
    		CNY2738.00

    secretin receptor 抗体 (E-9) Alexa Fluor® 790

    sc-166112 AF790
    200 µg/ml
    		CNY2738.00

    The data sheet says this antibody was raised against amino acids 33-121 mapping at the C-terminus of secretin of human origin. Secretin receptor (P47872) is a 440 aa protein. Where is the epitope? At the N-terminus?

    Asked by: akira
    Thank you for your question. secretin receptor (E-9) is a mouse monoclonal antibody raised against amino acids 33-121 mapping at the C-terminus of secretin of human origin.The epitope is DVLQVLWEEQDQCLQELSREQTGDLGTEQPVPGCEGMWDNISCWPSSVPGRMVEVECPRFLRMLTSRNGSLFRNCTQDGWSETFPRPNL Thank you.
    Answered by: Jiajun Z
    Date published: 2022-12-26

    Is is directed against the receptor or secretin itself? The datasheet does not give clear answers.

    Asked by: Loudi
    Thank you for your question. Anti-secretin receptor Antibody (E-9): sc-166112 recognizes secretin receptor, with protein accession number P47872.
    Answered by: Tech Support Europe
    Date published: 2021-09-23

    Is there any secretin antibody available specific for mouse/rat.If available can we inject this antibody by intra peritoneally/i.v./ in vivo in animal to check its binding to secretin receptor in vivo?

    Asked by: vijay modak
    Thank you for your question. At this time we have not validated any of our secretin receptor antibodies for reactivity with mouse or rat. We do not support any of our products for use in vivo or in live animal studies.
    Answered by: Technical Service
    Date published: 2017-04-24

    For Western Blot, is it recommended to use denatured or non-denatured conditions with secretin receptor (E-9): sc-166112 monoclonal antibody?

    Asked by: jenniferc
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 1 out of 5 by from Doesn't recognize the secretin receptorThis antibody does not recognize secretin receptor should be all ubiquitous in the gut villi epithelium but perhaps recognizes secretin.
    Date published: 2021-04-09
    Rated 4 out of 5 by from Good antibodyWorks as we expected. Tested by immunofluorescence.
    Date published: 2018-12-18
    Rated 4 out of 5 by from This antibody worksIn a western blot, the antibody worked well and thus we recommend it to others.
    Date published: 2018-01-19
    Rated 5 out of 5 by from Produced positive Western blot data of truncatedProduced positive Western blot data of truncated human recombinant secretin fusion protein. -SCBT QC
    Date published: 2014-05-08
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    secretin receptor 抗体 (E-9) is rated 3.5 out of 5 by 4.
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