Date published: 2025-9-7

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SAP 62 抗体 (4G8): sc-130563

5.0(2)
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说明书
  • SAP 62抗体(4G8)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 与SAP 62反应,从rat来源的肝细胞核中提取,在C-terminus进行表位图谱分析
  • 推荐用于 mouse, rat 和 human 来源的the native and denatured form of SAP 62 WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 SAP 62 抗体 (4G8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SAP 62 抗体 (4G8) 组合成套装提供。 (请参阅下面的订购信息)。

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    SAP 62,也称为SF3A2(剪接因子3A亚基2)、PRP11、PRPF11或SF3a66,是一种含有464个氨基酸的蛋白质,含有一个matrin型锌指结构,属于SF3A2家族。SAP 62定位于细胞核内,是SF3A剪接因子的一个亚基,SF3A是一个由三个亚基组成的异三聚体复合物,这三个亚基协同作用以介导U2 snRNP与前体mRNA中的分支点序列(BPS)的结合。SF3A复合物对于将15S U2 snRNP转化为活性17S蛋白质是必需的,后者直接在前体mRNA剪接事件中发挥作用。作为该复合物的第二亚基,SAP 62通过其N末端与亚基1(SAP 114)相互作用,同时通过其锌指结构域与15S U2 snRNP结合。除了其在RNA剪接中的作用外,SAP 62还被认为可以独立地作为微管结合蛋白发挥作用。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    SAP 62 抗体 (4G8)

    sc-130563
    200 µg/ml
    $316.00

    SAP 62 (4G8): m-IgG Fc BP-HRP 套装

    sc-539829
    200 µg Ab; 10 µg BP
    $354.00

    SAP 62 (4G8): m-IgG1 BP-HRP 套装

    sc-541736
    200 µg Ab; 20 µg BP
    $354.00

    Can SAP 62 (4G8): sc-130563 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Cweed
    Thank you for your question. Yes, SAP 62 (4G8): sc-130563 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Performs well in WesternPerforms well in Western, good signal with HeLa, A-431, K-562 and Hep G2 nuclear extracts. -SCBT QC
    Date published: 2015-06-05
    Rated 5 out of 5 by from Superb immunoperoxidase nuclear and cytoplasmicSuperb immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human cerebellum tissue. -SCBT QC
    Date published: 2015-04-11
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    SAP 62 抗体 (4G8) is rated 5.0 out of 5 by 2.
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