Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
ROR2 Antibody (H-1) - Western Blotting - Image 391028
ROR2 抗体 (H-1) Alexa Fluor® 680: sc-374174 AF680. U266 (A), K-562 (B) 和 T-47D (C) 全细胞裂解液中 ROR2 表达的直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.
ROR2 抗体 (H-1) Alexa Fluor® 680: sc-374174 AF680. U266 (A), K-562 (B) 和 T-47D (C) 全细胞裂解液中 ROR2 表达的直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.

ROR2 抗体 (H-1): sc-374174

4.8(8)
寫評論提問

说明书
  • ROR2 抗体 H-1 是小鼠单克隆 IgG1 κ，ROR2抗体, 在16篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列868-943位于human物种的ROR2的C-terminus
  • ROR2 抗体 (H-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 ROR2
  • 抗ROR2抗体（H-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ROR2 (H-1): sc-374174的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是ROR2 Antibody (H-1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与ROR2 Antibody (H-1) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

ROR2抗体（H-1）是一种IgG1 κ小鼠单克隆ROR2抗体（也被称为NTRKR2抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的ROR2蛋白。ROR2抗体（H-1）既有非偶联的抗ROR2抗体形式，也有多种偶联形式的抗ROR2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。ROR2（受体酪氨酸激酶样孤儿受体2），也被称为神经营养性酪氨酸激酶受体相关2（NTRKR2），是一种单次跨膜酪氨酸蛋白激酶受体。它包含一个细胞质酪氨酸激酶结构域、远端定位的富含丝氨酸-苏氨酸的结构域、一个细胞外免疫球蛋白样结构域、一个富含半胱氨酸的结构域和一个kringle结构域。ROR2对骨骼和内分泌发育非常重要，是软骨和生长板发育所必需的。它促进成骨细胞的分化，并在软骨细胞的早期形成中发挥重要作用。ROR2与Dlxin-1结合并关联，影响Msx-2的转录功能。ROR2还与典型的Wnt1和Wnt3相互作用，调节它们的信号通路。ROR2的缺陷可导致常染色体显性骨骼疾病，即B1型短指症，或常染色体隐性骨骼疾病，即Robinow综合征。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ROR2 抗体 (H-1) 参考文献:

  1. 隐性罗宾诺综合征（Robinow Syndrome）与显性手足畸形 B 型等全基因，是由 ROR2 基因突变引起的。  |  Afzal, AR., et al. 2000. Nat Genet. 25: 419-22. PMID: 10932186
  2. 受体酪氨酸激酶基因 ROR2 的不同突变会导致 Brachydactyly B 型。  |  Schwabe, GC., et al. 2000. Am J Hum Genet. 67: 822-31. PMID: 10986040
  3. 受体酪氨酸激酶Ror2与黑色素瘤相关抗原（MAGE）家族蛋白Dlxin-1结合，并调节其在细胞内的分布。  |  Matsuda, T., et al. 2003. J Biol Chem. 278: 29057-64. PMID: 12754255
  4. 一个基因，两种表型：常染色体隐性遗传罗宾诺综合征和常染色体显性遗传手足畸形 B 型的 ROR2 基因突变。  |  Afzal, AR. and Jeffery, S. 2003. Hum Mutat. 22: 1-11. PMID: 12815588
  5. 受体酪氨酸激酶 Ror2 参与了非经典 Wnt5a/JNK 信号通路。  |  Oishi, I., et al. 2003. Genes Cells. 8: 645-54. PMID: 12839624
  6. 孤儿受体酪氨酸激酶 Ror2 可调节成骨细胞中的典型 Wnt 信号转导。  |  Billiard, J., et al. 2005. Mol Endocrinol. 19: 90-101. PMID: 15388793
  7. 中枢神经元的神经元延伸：受体酪氨酸激酶 Ror1 和 Ror2 的新作用  |  Paganoni, S. and Ferreira, A. 2005. J Cell Sci. 118: 433-46. PMID: 15654020
  8. 孤儿受体酪氨酸激酶 Ror2 可促进成骨细胞分化并增强体内外骨形成。  |  Liu, Y., et al. 2007. Mol Endocrinol. 21: 376-87. PMID: 17095577
  9. 由骨形态发生蛋白拮抗剂 NOGGIN 的点突变引起的一种新的 B 型手足畸形亚型。  |  Lehmann, K., et al. 2007. Am J Hum Genet. 81: 388-96. PMID: 17668388
  10. ROR2敲除通过PI3K/ATK信号传导抑制乳腺癌生长。  |  Guo, M., et al. 2020. Aging (Albany NY). 12: 13115-13127. PMID: 32614787
  11. 在髓母细胞瘤进展中，miR-124-3p和miR-194-5p通过ROR2调节PI3K/AKT通路。  |  Wang, C., et al. 2024. Cancer Gene Ther. 31: 941-954. PMID: 38632356
  12. ROR2通过激活PI3K/AKT/mTOR信号传导促进三阴性乳腺癌细胞的侵袭和耐药性。  |  DA, X., et al. 2024. Oncol Res. 32: 1209-1219. PMID: 38948021

订购信息

产品名称产品编号规格价格数量收藏夹

ROR2 抗体 (H-1)

sc-374174
200 µg/ml
RMB2377.00

ROR2 (H-1): m-IgG1 BP-HRP 套装

sc-531710
200 µg Ab; 20 µg BP
RMB2662.00

ROR2 抗体 (H-1) AC

sc-374174 AC
500 µg/ml, 25% agarose
RMB3129.00

ROR2 抗体 (H-1) HRP

sc-374174 HRP
200 µg/ml
RMB2377.00

ROR2 抗体 (H-1) FITC

sc-374174 FITC
200 µg/ml
RMB2482.00

ROR2 抗体 (H-1) PE

sc-374174 PE
200 µg/ml
RMB2580.00

ROR2 抗体 (H-1) Alexa Fluor® 488

sc-374174 AF488
200 µg/ml
RMB2685.00

ROR2 抗体 (H-1) Alexa Fluor® 546

sc-374174 AF546
200 µg/ml
RMB2685.00

ROR2 抗体 (H-1) Alexa Fluor® 594

sc-374174 AF594
200 µg/ml
RMB2685.00

ROR2 抗体 (H-1) Alexa Fluor® 647

sc-374174 AF647
200 µg/ml
RMB2685.00

ROR2 抗体 (H-1) Alexa Fluor® 680

sc-374174 AF680
200 µg/ml
RMB2685.00

ROR2 抗体 (H-1) Alexa Fluor® 790

sc-374174 AF790
200 µg/ml
RMB2685.00

I tried to do Immunofluorescence on mouse lung tumor cell lines and tissue, and it did not work.

Asked by: Mane
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-03-13

What is the recommended fixation method for immunofluorescence staining with ROR2 (H-1): sc-374174 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_6, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_374174, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 165ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from SuperbThis is a very strong antibody that will give you nice solid bands in your Western blot. Reliable and consistent, you can't go wrong!Using 1:500 dilution I could make it work in bladder carcinoma cells.
Date published: 2022-11-12
Rated 5 out of 5 by from Very good antibodyWe started with the trial size, and it worked beautifully in IF with 4% Formaldehyde, and also in western blot, 1/250 dilution. We have now ordered the full size.
Date published: 2020-03-04
Rated 5 out of 5 by from Work well in WBI've got a 10ug sample of this antibody. It works well in WB at 1:200, MDA-MB-231 cells and T47D cells.
Date published: 2017-09-19
Rated 4 out of 5 by from Works on WBI tested the antibody on 20ug HEK293T and mIMCD3 cell lysate at 1:500 dilution. It works, although the signal is low for IMCD3. It also works for IF when Ror2 is overexpressed.
Date published: 2017-08-21
Rated 5 out of 5 by from Western BlotThe ror2 antibody worked quite well with our hela cells.
Date published: 2017-08-14
Rated 5 out of 5 by from Strong signalROR2 Antibody (H-1) produced strong signal by Western blot and IF
Date published: 2017-01-27
Rated 5 out of 5 by from Beautiful immunofluorescence membrane stainingBeautiful immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-29
Rated 5 out of 5 by from Produced positive Western Blot data of ROR2Produced positive Western Blot data of ROR2 expression in K-562 whole cell lysate. -SCBT QC
Date published: 2013-10-09
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_374174, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 11ms
  • REVIEWS, PRODUCT
ROR2 抗体 (H-1) is rated 4.8 out of 5 by 8.
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_374174, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。