Date published: 2025-9-7

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RNase H1 抗体 (S-34): sc-101114

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说明书
  • RNase H1抗体(S-34)是小鼠单克隆IgG2a (kappa轻链),规格为100 µg/ml
  • 免疫human物种的重组RNase H1
  • 推荐用于 mouse, rat 和 human 来源的RNase H1 WB, IP 和 ELISA检测
  • 目前,我们还没有完成RNase H1 抗体 (S-34)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    人RNase H1酶是一种细胞质内切酶,可降解RNA-DNA杂合体的RNA,产生5'-磷酸单酯产物。Mn2+和N-乙基马来酰亚胺可以抑制Mg2+依赖的RNase H1活性。RNase H1基因在所有人类细胞和组织中都以相似的水平存在,表明RNase H1可能是一种管家蛋白。人RNase H1基因定位于2p25.3染色体上,假基因存在于17p11.2染色体和1q染色体上。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    RNase H1 抗体 (S-34) 参考文献:

    1. RNase H1的"双面人格"及其在线粒体DNA代谢中的多重作用。  |  Holt, IJ. 2019. DNA Repair (Amst). 84: 102630. PMID: 31178343
    2. RNase H1和H2在处理RNA-DNA杂合体时受到不同的调控。  |  Lockhart, A., et al. 2019. Cell Rep. 29: 2890-2900.e5. PMID: 31775053
    3. 催化活性不强的纯化RNase H1:一种用于RNA-DNA杂交成像的特异且灵敏的探针。  |  Crossley, MP., et al. 2021. J Cell Biol. 220: PMID: 34232287
    4. RNA修饰会影响RNase H1介导的PS-ASO活性。  |  Doxtader Lacy, KA., et al. 2022. Mol Ther Nucleic Acids. 28: 814-828. PMID: 35664704
    5. RNase H1杂合体结合域工具用于哺乳动物细胞中DNA-RNA杂合体的细胞生物学研究。  |  Silva, S., et al. 2022. Methods Mol Biol. 2528: 115-125. PMID: 35704188
    6. NAT10 和 DDX21 蛋白与 RNase H1 相互作用并影响硫代磷酸酯寡核苷酸的性能  |  Zhang, L., et al. 2022. Nucleic Acid Ther. 32: 280-299. PMID: 35852833
    7. 哺乳动物RNase H1指导RNA引物的形成,从而启动线粒体DNA复制,并且对于线粒体DNA复制的完成也是必需的。  |  Misic, J., et al. 2022. Nucleic Acids Res. 50: 8749-8766. PMID: 35947649
    8. RNase H1通过在减数分裂期间降解DNA-RNA杂交物来促进重组酶的招募。  |  Liu, C., et al. 2023. Nucleic Acids Res. 51: 7357-7375. PMID: 37378420
    9. ARID1A-METTL3-m6A轴确保RNase H1有效介导R-loop的分解和基因组的稳定性。  |  Zhang, J., et al. 2024. Cell Rep. 43: 113779. PMID: 38358891
    10. RPA将RNase H1转化为双向外切核糖核酸酶,用于RNA-DNA杂合体的连续性切割。  |  Li, Y., et al. 2024. Nat Commun. 15: 7464. PMID: 39198528

    订购信息

    产品名称产品编号规格价格数量收藏夹

    RNase H1 抗体 (S-34)

    sc-101114
    100 µg/ml
    RMB2504.00

    For Western Blot, is it recommended to use denatured or non-denatured conditions with RNase H1 (S-34): sc-101114 antibody?

    Asked by: jenniferc
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 5 out of 5 by from Positive results in western blot using humanPositive results in western blot using human RNase H1 transfected 293T and HeLa whole cell lysates. -SCBT QC
    Date published: 2014-10-01
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    RNase H1 抗体 (S-34) is rated 5.0 out of 5 by 1.
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