Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

RNase H1 抗体 (H-4): sc-376326

5.0(2)
寫評論提問

说明书
  • RNase H1 抗体 H-4 是小鼠单克隆 IgG1 κ,RNase H1抗体, 在3篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的RNase H1的C-terminus附近的氨基酸241-275之间
  • RNase H1 抗体 (H-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 RNase H1; 也和以下物种反应,包括: canine
  • 抗RNase H1抗体(H-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RNase H1 (H-4): sc-376326免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是RNase H1 Antibody (H-4) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与RNase H1 Antibody (H-4) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

快捷链接

関連項目

RNase H1抗体(H-4)是一种IgG1 κ鼠单克隆RNase H1抗体(也称为RNase H1抗体),它通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类的RNase H1蛋白。RNase H1抗体(H-4)既可以作为非共轭抗RNase H1抗体形式提供,也可以作为多种共轭形式的抗RNase H1抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。人类RNase H1酶是一种细胞质内切核酸酶,可降解RNA-DNA杂交体中的RNA,产生5'-磷酸单酯产物。Mn2+和N-乙基马来酰亚胺可抑制Mg2+依赖性的RNase H1活性。RNase H1基因在所有的人类细胞和组织中以相似的水平存在,这表明RNase H1可能是一种管家蛋白。人类RNase H1基因定位于2p25.3染色体上,假基因位于17p11.2和1q染色体上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RNase H1 抗体 (H-4) 参考文献:

  1. 通过定点突变研究人类RNase H1的结构。  |  Wu, H., et al. 2001. J Biol Chem. 276: 23547-53. PMID: 11319219
  2. 核糖核酸酶 H1 位于第 2 号染色体上,在人类基因组中至少有三个假基因位点。  |  ten Asbroek, AL., et al. 2002. Genomics. 79: 818-23. PMID: 12036296
  3. 人类 RNase H1 的活性受相邻半胱氨酸之间形成的独特氧化还原开关调节。  |  Lima, WF., et al. 2003. J Biol Chem. 278: 14906-12. PMID: 12473655
  4. 人类 RNase H1 使用一个色氨酸和两个赖氨酸将酶定位在杂合双链底物的 3'-DNA/5'-RNA 末端。  |  Lima, WF., et al. 2003. J Biol Chem. 278: 49860-7. PMID: 14506260
  5. 确定人类 RNase H1 在 DNA 类反义药物药理学中的作用。  |  Wu, H., et al. 2004. J Biol Chem. 279: 17181-9. PMID: 14960586
  6. 人类 RNase H1 催化异质双链底物催化位点的结构要求。  |  Lima, WF., et al. 2004. J Biol Chem. 279: 36317-26. PMID: 15205459
  7. RNase H1在立体规则硼烷磷酸酯DNA/RNA杂合体上的活性结构基础。  |  Johnson, CN., et al. 2011. Biochemistry. 50: 3903-12. PMID: 21443203
  8. 极端嗜盐古细菌Halobacterium sp. NRC-1的RNase H1二价金属离子诱导折叠机制。  |  Tannous, E. and Kanaya, S. 2014. PLoS One. 9: e109016. PMID: 25268753
  9. 由于缺乏RNase H1,引物的保留对于线粒体DNA复制来说是灾难性的。  |  Holmes, JB., et al. 2015. Proc Natl Acad Sci U S A. 112: 9334-9. PMID: 26162680
  10. 哺乳动物 RNase H1 对 RNAP I 转录 R 环的动态核质和核仁定位。  |  Shen, W., et al. 2017. Nucleic Acids Res. 45: 10672-10692. PMID: 28977560
  11. 人 RNase H 的 cDNA 分子克隆和表达。  |  Wu, H., et al. 1998. Antisense Nucleic Acid Drug Dev. 8: 53-61. PMID: 9512096
  12. 与细菌 RNase HI 有关的人和小鼠核糖核酸酶 H 的克隆, 表达和绘图。  |  Cerritelli, SM. and Crouch, RJ. 1998. Genomics. 53: 300-7. PMID: 9799596

订购信息

产品名称产品编号规格价格数量收藏夹

RNase H1 抗体 (H-4)

sc-376326
200 µg/ml
RMB2377.00

RNase H1 (H-4): m-IgGκ BP-HRP 套装

sc-522915
200 µg Ab, 40 µg BP
RMB2662.00

RNase H1 抗体 (H-4) AC

sc-376326 AC
500 µg/ml, 25% agarose
RMB3129.00

RNase H1 抗体 (H-4) HRP

sc-376326 HRP
200 µg/ml
RMB2377.00

RNase H1 抗体 (H-4) FITC

sc-376326 FITC
200 µg/ml
RMB2482.00

RNase H1 抗体 (H-4) PE

sc-376326 PE
200 µg/ml
RMB2580.00

RNase H1 抗体 (H-4) Alexa Fluor® 488

sc-376326 AF488
200 µg/ml
RMB2685.00

RNase H1 抗体 (H-4) Alexa Fluor® 546

sc-376326 AF546
200 µg/ml
RMB2685.00

RNase H1 抗体 (H-4) Alexa Fluor® 594

sc-376326 AF594
200 µg/ml
RMB2685.00

RNase H1 抗体 (H-4) Alexa Fluor® 647

sc-376326 AF647
200 µg/ml
RMB2685.00

RNase H1 抗体 (H-4) Alexa Fluor® 680

sc-376326 AF680
200 µg/ml
RMB2685.00

RNase H1 抗体 (H-4) Alexa Fluor® 790

sc-376326 AF790
200 µg/ml
RMB2685.00

RNase H1 (H-4) 中和勝肽

sc-376326 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-376326 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24

Why do the untransfected 293T cells (sample western blot data above, panel 3) show no RNase H expression? RNase H expression is ubiquitous.

Asked by: JonnyWattsUp
Thank you for your question. We have tested a variety of different RNase H1 antibodies, including our mouse monoclonal, RNase H1 (H-4): sc-376326, in untransfected and transfected 293T cells and the results typically do not show a signal detected by Western blot in the untransfected 293T cell lysate. This could be for a variety of reasons. Although RNase H protein expression is ubiquitous, it may not be expressed abundantly enough in 293T to be detected by Western blot. Also, the purpose of our Western blot tests was to evaluate the antibody by identifying positive upregulation of the protein in the transfected cell lysate. Therefore, our Western blot conditions used were not optimized to detect signal in the untransfected cells. For example, the amount of protein loaded on the gels, our antibody incubation times, and the exposure times during the ECL reaction, all could be optimized if the goal was to detect endogenous protein in the untransfected control lysate.
Answered by: Technical Support
Date published: 2017-01-19
  • y_2025, m_8, d_31, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_376326, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Nice Western Blot data of RNase H1 expressionNice Western Blot data of RNase H1 expression in non-transfected and mouse RNase H1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Great Western Blot data of RNase H1 expressionGreat Western Blot data of RNase H1 expression in Jurkat and PC-12 whole cell lysates. -SCBT QC
Date published: 2013-08-14
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_376326, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
RNase H1 抗体 (H-4) is rated 5.0 out of 5 by 2.
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_376326, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 113ms
  • REVIEWS, PRODUCT