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RNase H1 抗体 (H-9): sc-365267

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说明书
  • RNase H1抗体(H-9)是小鼠单克隆IgG3 (kappa轻链),规格为200 µg/ml
  • 特异性抗原位于human物种的RNase H1的C-terminus的氨基酸253-278之间
  • 推荐用于 mouse, rat 和 human 来源的RNase H1 WB, IP, IF 和 ELISA检测
  • m-IgG3 BP-HRP是RNase H1 Antibody (H-9) 适用于 WB 应用。 的首选辅助检测试剂,该试剂现与RNase H1 Antibody (H-9) 搭配使用(请参阅下面的订购信息)。

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    関連項目

    人RNase H1酶是一种细胞质内切酶,可降解RNA-DNA杂合体的RNA,产生5'-磷酸单酯产物。Mn2+和N-乙基马来酰亚胺可以抑制Mg2+依赖的RNase H1活性。RNase H1基因在所有人类细胞和组织中都以相似的水平存在,表明RNase H1可能是一种管家蛋白。人RNase H1基因定位于2p25.3染色体上,假基因存在于17p11.2染色体和1q染色体上。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    RNase H1 抗体 (H-9) 参考文献:

    1. 来自宏基因组的LC11-RNase H1(硫硫杆菌RNase H1的同源物)的活性、稳定性和结构。  |  Nguyen, TN., et al. 2012. Protein Sci. 21: 553-61. PMID: 22389131
    2. 人类 RNase H1 与蛋白 P32 有关,参与线粒体前 RNA 处理。  |  Wu, H., et al. 2013. PLoS One. 8: e71006. PMID: 23990920
    3. 依赖于 RNase H1 的反义寡核苷酸在引导细胞质和细胞核中的 RNA 切割方面具有强大的活性。  |  Liang, XH., et al. 2017. Mol Ther. 25: 2075-2092. PMID: 28663102
    4. 人类线粒体OriL的引物去除需要一条包含RNase H1的二核酸酶途径。  |  Al-Behadili, A., et al. 2018. Nucleic Acids Res. 46: 9471-9483. PMID: 30102370
    5. RNase H1在人类线粒体中引导DNA复制的起源特异性启动。  |  Posse, V., et al. 2019. PLoS Genet. 15: e1007781. PMID: 30605451
    6. RNase H1通过降解7S RNA来调节线粒体的转录和翻译。  |  Reyes, A., et al. 2019. Front Genet. 10: 1393. PMID: 32082360
    7. 拟南芥中RNase H1样蛋白的进化历史和活性。  |  Kuciński, J., et al. 2020. Plant Cell Physiol. 61: 1107-1119. PMID: 32191307
    8. 硫代磷酸酯寡核苷酸与 RNase H1 结合会导致蛋白质构象发生变化,并改变 RNase H1 与其他蛋白质的相互作用。  |  Zhang, L., et al. 2021. Nucleic Acids Res. 49: 2721-2739. PMID: 33577678
    9. 逆转录酶逆转录终止和噬菌体防御依赖于宿主RNase H1。  |  Palka, C., et al. 2022. Nucleic Acids Res. 50: 3490-3504. PMID: 35293583
    10. CHCHD2/Sirt1共抑制因子参与G9a介导的RNase H1表达调控,从而控制R-loop。  |  Li, L., et al. 2023. Cell Insight. 2: 100112. PMID: 37388553

    订购信息

    产品名称产品编号规格价格数量收藏夹

    RNase H1 抗体 (H-9)

    sc-365267
    200 µg/ml
    RMB2377.00

    RNase H1 (H-9): m-IgG3 BP-HRP 套装

    sc-550422
    200 µg Ab; 40 µg BP
    RMB2662.00

    RNase H1 (H-9) 中和勝肽

    sc-365267 P
    100 µg/0.5 ml
    RMB511.00

    What application is the blocking peptide sc-365267 P appropriate for?

    Asked by: DefinitelyNotMatt
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Good Western blot data of RNase H1 expressionGood Western blot data of RNase H1 expression in non-transfected and mouse RNase H1 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2013-07-03
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    RNase H1 抗体 (H-9) is rated 5.0 out of 5 by 1.
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