Date published: 2025-9-7

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RNase H1 抗体 (C-8): sc-365057

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说明书
  • RNase H1抗体(C-8)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的RNase H1的C-terminus的氨基酸253-278之间
  • 推荐用于 mouse, rat 和 human 来源的RNase H1 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是RNase H1 Antibody (C-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与RNase H1 Antibody (C-8) 打包提供(请参阅下面的订购信息)。

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関連項目

人RNase H1酶是一种细胞质内切酶,可降解RNA-DNA杂合体的RNA,产生5'-磷酸单酯产物。Mn2+和N-乙基马来酰亚胺可以抑制Mg2+依赖的RNase H1活性。RNase H1基因在所有人类细胞和组织中都以相似的水平存在,表明RNase H1可能是一种管家蛋白。人RNase H1基因定位于2p25.3染色体上,假基因存在于17p11.2染色体和1q染色体上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RNase H1 抗体 (C-8) 参考文献:

  1. 人类 RNase H1 的活性受相邻半胱氨酸之间形成的独特氧化还原开关调节。  |  Lima, WF., et al. 2003. J Biol Chem. 278: 14906-12. PMID: 12473655
  2. 元基因组衍生的LC11-RNase H1与RNA/DNA混合物的复合物的晶体结构。  |  Nguyen, TN., et al. 2013. J Struct Biol. 182: 144-54. PMID: 23500886
  3. RNase H1在DNA修复中的作用:与RNase H2合作去除DNA中错误插入的单核苷酸。  |  Tannous, E., et al. 2015. Sci Rep. 5: 9969. PMID: 25951507
  4. 分枝杆菌RNase H酶之间的分工:RnhA或RnhC的RNase H1活性对于生长至关重要,而RnhB和RnhA则防止在静止期被过氧化氢杀死。  |  Gupta, R., et al. 2017. Nucleic Acids Res. 45: 1-14. PMID: 27899559
  5. RNase H1与DNA Gyrases合作,限制R-Loop并维持拟南芥叶绿体基因组的完整性。  |  Yang, Z., et al. 2017. Plant Cell. 29: 2478-2497. PMID: 28939594
  6. 翻译会影响 RNase H1 依赖性寡核苷酸靶向 mRNA 的反义活性。  |  Liang, XH., et al. 2018. Nucleic Acids Res. 46: 293-313. PMID: 29165591
  7. PS-ASO/蛋白质与P54nrb和RNase H1相互作用的动力学和亚细胞分析。  |  Vickers, TA., et al. 2019. Nucleic Acids Res. 47: 10865-10880. PMID: 31495875
  8. 线粒体RNase H1活性调节R-loop稳态,以维持基因组完整性并促进拟南芥早期胚胎发育。  |  Cheng, L., et al. 2021. PLoS Biol. 19: e3001357. PMID: 34343166
  9. RNase H1将环形内含RNA降解与转录物功能联系起来。  |  Li, X., et al. 2021. Sci China Life Sci. 64: 1795-1809. PMID: 34453665
  10. α-羟基三酮类化合物是人体RNase H1的非竞争性抑制剂,可与活性位点结合并调节底物结合。  |  Ponzar, NL., et al. 2022. J Biol Chem. 298: 101790. PMID: 35247386

订购信息

产品名称产品编号规格价格数量收藏夹

RNase H1 抗体 (C-8)

sc-365057
200 µg/ml
RMB2377.00

RNase H1 (C-8): m-IgG Fc BP-HRP 套装

sc-537769
200 µg Ab; 10 µg BP
RMB2662.00

RNase H1 (C-8): m-IgGκ BP-HRP 套装

sc-535142
200 µg Ab; 40 µg BP
RMB2662.00

RNase H1 (C-8): m-IgG1 BP-HRP 套装

sc-545341
200 µg Ab; 20 µg BP
RMB2662.00

RNase H1 (C-8) 中和勝肽

sc-365057 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-365057 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent immunofluorescence cytoplasmicExcellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Great Western Blot data of RNase H1 expressionGreat Western Blot data of RNase H1 expression in non-transfected and mouse RNase H1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-10-05
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RNase H1 抗体 (C-8) is rated 5.0 out of 5 by 2.
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