Date published: 2025-9-7

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RKIP 抗体 (8): sc-101504

5.0(5)
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说明书
  • RKIP抗体(8)是小鼠单克隆IgG2b κ, 在4篇文献中引用,规格为200 µg/ml
  • 免疫human物种的重组RKIP
  • 推荐用于 mouse, rat 和 human 来源的RKIP WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 是 RKIP 抗体 (8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RKIP 抗体 (8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Raf激酶抑制蛋白(RKIP)是一种胞质蛋白,最初被鉴定为在脑组织中表达的磷脂酰乙醇胺结合蛋白(PBP),并从睾丸液中分泌。此外,RKIP是通过酵母双杂交筛选人T细胞库而鉴定出的,该库旨在鉴定与丝氨酸/苏氨酸激酶Raf-1的BXB激酶结构域相关的蛋白。随后的体外和体内研究表明,RKIP与Raf-1的活性形式和非活性形式结合,从而调节MAP激酶通路的信号级联。RKIP与激酶活性Raf-1的特异性结合竞争性抑制Raf-1底物MEK的结合和激活。反过来,RKIP通过减少MEK效应蛋白(包括ERK1和ERK2)的激活以及随后诱导AP-1介导的转录来影响下游MAP激酶信号传导。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RKIP 抗体 (8) 参考文献:

  1. 食管癌及其转移中RAF激酶抑制蛋白(RKIP)的下调。  |  Birner, P., et al. 2012. Clin Exp Metastasis. 29: 551-9. PMID: 22460832
  2. 多种癌症中Raf激酶抑制蛋白(RKIP)的调查。  |  Lamiman, K., et al. 2014. Crit Rev Oncog. 19: 455-68. PMID: 25597355
  3. Raf激酶抑制蛋白在幽门螺杆菌介导的胃癌信号传导中的作用。  |  Nisimova, L., et al. 2014. Crit Rev Oncog. 19: 469-81. PMID: 25597356
  4. 人类肠道Raf激酶抑制蛋白(RKIP)催化普拉斯格雷作为生物活化水解酶。  |  Kazui, M., et al. 2016. Drug Metab Dispos. 44: 115-23. PMID: 26558823
  5. Raf激酶抑制蛋白通过NF-κB途径减轻缺血诱导的微胶质细胞凋亡和激活。  |  Su, L., et al. 2017. Cell Physiol Biochem. 41: 1125-1134. PMID: 28245468
  6. Raf激酶抑制蛋白与癌症干细胞转录因子(Oct4、KLF4、Sox2、Nanog)之间的相互作用。  |  Lee, S., et al. 2017. Tumour Biol. 39: 1010428317692253. PMID: 28378634
  7. Raf激酶抑制蛋白优先促进TLR3引发的信号传导和炎症。  |  Lai, R., et al. 2017. J Immunol. 198: 4086-4095. PMID: 28411188
  8. 骨髓性白血病发生中的Raf激酶抑制蛋白。  |  Zebisch, A., et al. 2019. Int J Mol Sci. 20: PMID: 31744053
  9. 结核分枝杆菌Raf激酶抑制蛋白(RKIP)Rv2140c通过磷酸化参与细胞壁阿拉伯半乳聚糖的生物合成。  |  Abo-Kadoum, MA., et al. 2021. Microbiol Res. 242: 126615. PMID: 33189070
  10. Raf激酶抑制蛋白与磷酸化细胞外信号调节激酶联合使用,为胃肠道间质瘤提供了有价值的预后信息。  |  Qu, WZ., et al. 2023. World J Gastroenterol. 29: 4200-4213. PMID: 37475847

订购信息

产品名称产品编号规格价格数量收藏夹

RKIP 抗体 (8)

sc-101504
200 µg/ml
RMB2377.00

RKIP (8): m-IgG Fc BP-HRP 套装

sc-539768
200 µg Ab; 10 µg BP
RMB2662.00

RKIP (8): m-IgG2b BP-HRP 套装

sc-549748
200 µg Ab; 10 µg BP
RMB2662.00

I am using RKIP (8): sc-101504 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 功能不错Single strong band in WB in human transfected 293 whole cell lysates
Date published: 2019-03-16
Rated 5 out of 5 by from Great antibodyPurchased the RKIP Antibody (8), and as received a sample of the secondary antibody, mouse IgG kappa - HRP. Strong signal is seen at the expected size (23 kDa) in MCF7 cells and other cell lines using the provided secondary.
Date published: 2016-12-13
Rated 5 out of 5 by from Band detected at expected mw in 293T andBand detected at expected mw in 293T and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-01-08
Rated 5 out of 5 by from WB publishable data with HNE1 and HuvecWB publishable data with HNE1 and Huvec - SCBT Publication Review
Date published: 2014-08-03
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RKIP 抗体 (8) is rated 5.0 out of 5 by 5.
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