Date published: 2025-9-7

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RKIP 抗体 (H-10): sc-376925

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说明书
  • RKIP 抗体 H-10 是小鼠单克隆 IgG1RKIP抗体, 在9篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长RKIP的氨基酸1-187
  • RKIP 抗体 (H-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 RKIP and processed active peptide HCNP
  • 抗RKIP抗体(H-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RKIP (H-10): sc-376925免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 RKIP 抗体 (H-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与RKIP 抗体 (H-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

RKIP抗体(H-10)是一种IgG1小鼠单克隆RKIP抗体(也称为磷脂酰乙醇胺结合蛋白1抗体、PEBP抗体、海马胆碱能神经刺激肽抗体、Raf激酶抑制蛋白抗体、前列腺结合蛋白抗体、神经多肽H3抗体、HCNPpp抗体、PEBP-1抗体、HCNP抗体或PBP抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源RKIP蛋白。RKIP抗体(H-10)既可以是非偶联的抗RKIP抗体形式,也可以是多种偶联形式的抗RKIP抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Raf激酶抑制蛋白(RKIP)是一种胞质蛋白,最初被鉴定为在脑组织中表达的磷脂酰乙醇胺结合蛋白(PBP),并从睾丸液中分泌。此外,RKIP是通过酵母双杂交筛选人T细胞库鉴定的,旨在鉴定与丝氨酸/苏氨酸激酶Raf-1的BXB激酶结构域相关的蛋白。随后的体外和体内研究表明,RKIP与Raf-1的活性形式和非活性形式结合,从而调节MAP激酶通路的信号级联。RKIP与激酶活性Raf-1的特异性结合竞争性抑制Raf-1底物MEK的结合和激活。反过来,RKIP通过减少MEK效应蛋白(包括ERK1和ERK2)的激活以及随后诱导AP-1介导的转录来影响下游MAP激酶信号传导。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RKIP 抗体 (H-10) 参考文献:

  1. RKIP 对 Raf-1 激酶活性和 MAP 激酶信号的抑制。  |  Yeung, K., et al. 1999. Nature. 401: 173-7. PMID: 10490027
  2. Raf激酶抑制蛋白(RKIP)结合口袋的表征:基于核磁共振的筛选可识别小分子配体。  |  Shemon, AN., et al. 2010. PLoS One. 5: e10479. PMID: 20463977
  3. 磷酸化Raf激酶抑制蛋白(pRKIP)的表达是肺癌存活的预测指标。  |  Huerta-Yepez, S., et al. 2011. BMC Cancer. 11: 259. PMID: 21689459
  4. Raf激酶抑制蛋白(RKIP)缺乏可缩短前列腺癌小鼠遗传模型的肿瘤发生潜伏期并增加转移。  |  Escara-Wilke, J., et al. 2015. Prostate. 75: 292-302. PMID: 25327941
  5. Raf 激酶抑制蛋白通过 NF-κB 和 ERK 通路调节氧-葡萄糖剥夺诱导的 PC12 细胞凋亡。  |  Su, L., et al. 2016. J Clin Biochem Nutr. 59: 86-92. PMID: 27698534
  6. Raf激酶抑制蛋白(RKIP)通过抑制(核转录因子-κB(NF-κB)通路)抑制肿瘤坏死因子-α(TNF-α)诱导的血管平滑肌细胞粘附分子表达。  |  Jing, SH., et al. 2017. Med Sci Monit. 23: 4789-4797. PMID: 28983072
  7. Raf激酶抑制蛋白与程序性细胞死亡配体1在癌症中的表达之间的相互影响:在免疫逃逸中的作用和治疗意义。  |  Ho, M. and Bonavida, B. 2024. Cells. 13: PMID: 38786085
  8. 大鼠和人类 HCNP 前体蛋白, 牛磷脂酰乙醇胺结合蛋白和大鼠 23 kDa 蛋白与阿片结合蛋白的序列同源性。  |  Tohdoh, N., et al. 1995. Brain Res Mol Brain Res. 30: 381-4. PMID: 7637590
  9. Raf-1 和 MEKK 对 ERK 和 JNK 丝裂原活化蛋白激酶的不同激活作用。  |  Minden, A., et al. 1994. Science. 266: 1719-23. PMID: 7992057
  10. 哺乳动物睾丸和附睾磷脂结合蛋白的序列分析及其在精子和细胞外分泌物中的分布。  |  Perry, AC., et al. 1994. Biochem J. 301 (Pt 1): 235-42. PMID: 8037677
  11. 通过单克隆抗体抑制 Raf-1 信号传导,干扰 Raf-1 激活和 Mek 底物结合。  |  Kolch, W., et al. 1996. Oncogene. 13: 1305-14. PMID: 8808705
  12. Raf-1 调节的复杂性。  |  Morrison, DK. and Cutler, RE. 1997. Curr Opin Cell Biol. 9: 174-9. PMID: 9069260

订购信息

产品名称产品编号规格价格数量收藏夹

RKIP 抗体 (H-10)

sc-376925
200 µg/ml
RMB2377.00

RKIP (H-10): m-IgG1 BP-HRP 套装

sc-531726
200 µg Ab; 20 µg BP
RMB2662.00

RKIP 抗体 (H-10) AC

sc-376925 AC
500 µg/ml, 25% agarose
RMB3129.00

RKIP 抗体 (H-10) HRP

sc-376925 HRP
200 µg/ml
RMB2377.00

RKIP 抗体 (H-10) FITC

sc-376925 FITC
200 µg/ml
RMB2482.00

RKIP 抗体 (H-10) PE

sc-376925 PE
200 µg/ml
RMB2580.00

RKIP 抗体 (H-10) Alexa Fluor® 488

sc-376925 AF488
200 µg/ml
RMB2685.00

RKIP 抗体 (H-10) Alexa Fluor® 546

sc-376925 AF546
200 µg/ml
RMB2685.00

RKIP 抗体 (H-10) Alexa Fluor® 594

sc-376925 AF594
200 µg/ml
RMB2685.00

RKIP 抗体 (H-10) Alexa Fluor® 647

sc-376925 AF647
200 µg/ml
RMB2685.00

RKIP 抗体 (H-10) Alexa Fluor® 680

sc-376925 AF680
200 µg/ml
RMB2685.00

RKIP 抗体 (H-10) Alexa Fluor® 790

sc-376925 AF790
200 µg/ml
RMB2685.00

Can RKIP (H-10): sc-376925 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, RKIP (H-10): sc-376925 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Good productOur team evaluated performance of this antibody by Western blot with use of following samples: HEK293T cell line (30µg), HEPG2 cell line (30 µg). Antibody seems to work properly.
Date published: 2021-06-08
Rated 5 out of 5 by from great featurein lm3 cell ,diluted 1:500,it is very clear,i am get it from free sample.
Date published: 2017-08-07
Rated 5 out of 5 by from Great for Western blotsHave used this antibody for Western blots and gotten strong bands, just as pictured above. Would recommend this one!
Date published: 2017-07-21
Rated 5 out of 5 by from wonderful antibody!1:500 diluted with bsa for the wb of rat protein which extracted from h9c2 cells,the band is strong .
Date published: 2017-06-19
Rated 5 out of 5 by from Great antibodyThe antibody is extremely strong and highly specific.
Date published: 2017-05-05
Rated 5 out of 5 by from Western BlottingThe antibody works well. Single strong band at 23 kDa.
Date published: 2017-02-06
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-05-19
Rated 4 out of 5 by from Strong band seen in WB using PCStrong band seen in WB using PC-12 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-04-29
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RKIP 抗体 (H-10) is rated 4.9 out of 5 by 8.
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