Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Ribosomal Protein L11 Antibody (2A1) - Immunohistochemistry - Image 346122
核糖体蛋白 L11 抗体 (2A1): sc-293224. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核定位.
Ribosomal Protein L11 Antibody (2A1) - Immunohistochemistry - Image 346122
核糖体蛋白 L11 抗体 (2A1): sc-293224. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核定位.
Ribosomal Protein L11 Antibody (2A1) - Western Blotting - Image 348824
核糖体蛋白 L11 抗体 (2A1): sc-293224. 人重组核糖体蛋白 L11 融合蛋白的 Western 印迹分析.
核糖体蛋白 L11 抗体 (2A1): sc-293224. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核定位.

Ribosomal Protein L11 抗体 (2A1): sc-293224

5.0(1)
寫評論提問

说明书
  • Ribosomal Protein L11 抗体 2A1 是小鼠单克隆 IgG2b (kappa轻链)，Ribosomal Protein L11抗体，规格为100 µg/ml
  • 免疫human物种的代表全长Ribosomal Protein L11的氨基酸1-177
  • 推荐用于 mouse, rat 和 human 来源的Ribosomal Protein L11 WB, IP, IF, IHC(P) 和 ELISA检测
  • 目前，我们还没有完成Ribosomal Protein L11 抗体 (2A1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

    快捷链接

    相关产品
    描述
    基因信息
    说明书与实验方案

    関連項目

    此外 Ribosomal Protein 抗体 包括 Ribosomal Protein L11, Ribosomal Protein L13, Ribosomal Protein L14, Ribosomal Protein L17, Ribosomal Protein L18, Ribosomal Protein L19, Ribosomal Protein L21, Ribosomal Protein L22, Ribosomal Protein L23a和Ribosomal Protein L30

    核糖体蛋白L11抗体（2A1）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的核糖体蛋白L11（RPL11）。抗RPL11抗体（2A1）以非结合形式提供。RPL11在核糖体组装和功能中起着至关重要的作用，对于所有活细胞中的蛋白质合成至关重要。RPL11（2A1）单克隆抗体定位于核仁，RPL11参与核糖体RNA的加工和成熟，这对于核糖体的形成至关重要。RPL11的功能仍然至关重要，因为核糖体生物发生过程的破坏会导致各种疾病，包括癌症和核糖体病。编码人类RPL11的基因位于1p36.1-p35染色体上，物种间的进化保护强调了RPL11在细胞生物学中的基础作用。RPL11 (2A1)抗体是研究核糖体功能和相关细胞过程的研究人员的宝贵工具。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Ribosomal Protein L11 抗体 (2A1) 参考文献:

    1. 通过原位杂交将 L11 核糖体蛋白基因（RPL11）分配到人类染色体 1p36.1-->p35。  |  Graphodatsky, AS., et al. 1999. Cytogenet Cell Genet. 84: 97-8. PMID: 10343117
    2. 核糖体蛋白 L11 负向调节肿瘤蛋白 MDM2，并介导 p53 依赖性核糖体应激检查点途径。  |  Zhang, Y., et al. 2003. Mol Cell Biol. 23: 8902-12. PMID: 14612427
    3. 7 个含内含子的核糖体蛋白基因的图谱显示，它们在人类基因组中是不相连的。  |  Feo, S., et al. 1992. Genomics. 13: 201-7. PMID: 1577483
    4. 大鼠核糖体蛋白的一级结构：L27a 和 L28 的氨基酸序列以及 S4 和 S12 序列的修正。  |  Wool, IG., et al. 1990. Biochim Biophys Acta. 1050: 69-73. PMID: 2207170
    5. 核糖体蛋白 L11 与肿瘤抑制因子 ARF 之间的物理和功能相互作用  |  Dai, MS., et al. 2012. J Biol Chem. 287: 17120-17129. PMID: 22467867
    6. 核糖体应激挑战皮层神经元对核糖体蛋白 L11 的促凋亡需求  |  Slomnicki, LP., et al. 2018. Mol Neurobiol. 55: 538-553. PMID: 27975169
    7. 鼠李糖核糖体蛋白基因 S6 的一级结构。  |  Gross, T., et al. 1988. Curr Genet. 13: 57-63. PMID: 2834104
    8. 核糖体蛋白 L11 选择性地稳定 GTPase 中心 rRNA 结构域的三级结构  |  Welty, R., et al. 2020. J Mol Biol. 432: 991-1007. PMID: 31874150
    9. 从克隆的 cDNA 中得到的人类核糖体蛋白 S6 的一级结构。  |  Heinze, H., et al. 1988. J Biol Chem. 263: 4139-44. PMID: 3279029
    10. 核极应激反应通过核糖体蛋白 L11 调节 P53 感染癌症的拓扑异构酶抑制剂敏感性  |  Ishihara, Y., et al. 2022. Int J Mol Sci. 23: PMID: 36555627
    11. 进化保守的核糖体蛋白 L11 甲基化对细菌严格响应的调控。  |  Walukiewicz, HE., et al. 2024. mBio. 15: e0177324. PMID: 39189746
    12. L5, L21, L27a, L28, S5, S9, S10 和 S29 人类核糖体蛋白 mRNA 的克隆, 测序和表达。  |  Frigerio, JM., et al. 1995. Biochim Biophys Acta. 1262: 64-8. PMID: 7772601

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Ribosomal Protein L11 抗体 (2A1)

    sc-293224
    100 µg/ml
    		RMB2377.00

    Why is your antibody recognising a 40 kDa protein - RPL11 is about 20 kDa. It should also be predominantly cytoplasmic - as that is where the ribosomes are. My lab works on ribosome biogenesis and I think this antibody should be removed from your stock

    Asked by: NickWatkins
    Thank you very much for the question! The full-length Ribosomal Protein L11 of human origin has 177 amino acids. The calculated molecular weight of this protein to be detected by standard WB techniques is expected to be around 20kDa. This is also the weight we refer to on the corresponding product datasheet you can download by clicking the following link: https://datasheets.scbt.com/sc-293224.pdf The WB image we show on this product page is showing a Ribosomal Protein L11 - GST fusion protein with the corresponding higher molecular weight. This is the correct mw size we expected in this control experiment but of course not the size to be detected on endogenously expressed Ribosomal Protein L11. In the IF staining of methanol-fixed HeLa cells our labs have performed this antibody was showing nuclear localization. According to the protein literature we found this protein should be expressed predominantly in the cytoplasma but we also found supporting information about IF localization in the nucleus. Our labs will look further into this and post additional data here on the product page upon availability.
    Answered by: Technical Support Europe
    Date published: 2017-09-18

    Why is the RPL11 signal in the nucleus when RPL11 is predominantly a cytoplasmic protein? This suggests to me that the antibody is non-functional

    Asked by: NickWatkins
    Thank you for your question. HPRD and UniProt list nuclear and nucleolar as subcellular localizations for RP11; links to their websites are provided below for reference. However, we will also ask our lab to reevaluate the data based on your feedback. If we are able to provide any new or revised data, it will be updated on our website. http://www.uniprot.org/uniprot/P62913#subcellular_location http://www.hprd.org/summary?hprd_id=10364&isoform_id=10364_1&isoform_name=Isoform_1
    Answered by: Technical Support
    Date published: 2017-08-01

    Can sc-293224: Ribosomal Protein L11 (2A1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Professor Griffin
    Thank you for your question. Yes, sc-293224: Ribosomal Protein L11 (2A1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-24
    • y_2025, m_8, d_30, h_9CST
    • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasquestionsanswers, tq_3
    • loc_zh_CN, sid_293224, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getContent, 139ms
    • QUESTIONS, PRODUCT
    Rated 5 out of 5 by from Good for Western BlotAntibody detects human recombinant Ribosomal Protein L11 fusion protein by Western Blot. -SCBT QC
    Date published: 2023-09-14
    • y_2025, m_8, d_30, h_9
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasreviews, tv_0, tr_1
    • loc_zh_CN, sid_293224, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getReviews, 14ms
    • REVIEWS, PRODUCT
    Ribosomal Protein L11 抗体 (2A1) is rated 5.0 out of 5 by 1.
    • y_2025, m_8, d_30, h_9
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasreviews, tv_0, tr_1
    • loc_zh_CN, sid_293224, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getAggregateRating, 98ms
    • REVIEWS, PRODUCT
    021-6093-6350
    访问 Santa Cruz Animal Health|访问 San Juan Ranch
    Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
    版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。