Date published: 2025-9-8

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RGS10 抗体 (A-8): sc-46679

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说明书
  • RGS10 抗体 A-8 是小鼠单克隆 IgG2b κ,RGS10抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫human物种的RGS10的氨基酸15-173
  • RGS10 抗体 (A-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 RGS10
  • 抗RGS10抗体(A-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RGS10 (A-8): sc-46679免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 RGS10 抗体 (A-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RGS10 抗体 (A-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

RGS10抗体(A-8)是一种鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测人类RGS10蛋白。RGS10 (A-8) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。RGS10蛋白在调节G蛋白信号通路中起着至关重要的作用,而G蛋白信号通路对于将信号从细胞表面受体传递到细胞内效应器至关重要。具体而言,RGS10作为某些G蛋白α亚基的GTP酶激活蛋白(GAP),可增强其将GTP水解为GDP的能力,从而促进信号通路失活。这种调节功能对于维持细胞内稳态和确保对外部刺激做出适当反应至关重要。RGS10失调与多种疾病有关,包括癌症和神经系统疾病,这凸显了RGS10作为潜在治疗靶点的重要性。抗RGS10抗体(A-8)具有多种应用,在G蛋白信号传导中发挥着关键作用,是研究人员研究细胞信号传导机制及其对健康和疾病的影响的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RGS10 抗体 (A-8) 参考文献:

  1. 不同的 beta 亚基决定了 G 蛋白与跨膜受体的相互作用。  |  Kleuss, C., et al. 1992. Nature. 358: 424-6. PMID: 1322501
  2. G 蛋白 gamma 亚基的选择性组织分布,包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
  3. 异三聚体 GTP 结合蛋白 beta 亚基的多样性:新型 beta 亚基 cDNA 的特征。  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
  4. Gustducin 是一种味觉细胞特异性 G 蛋白,与转导蛋白关系密切。  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
  5. 信号转导中 G 蛋白的多样性。  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
  6. 朗格汉斯细胞和其他表达朗格林的树突状细胞的起源、稳态和功能。  |  Merad, M., et al. 2008. Nat Rev Immunol. 8: 935-47. PMID: 19029989
  7. 朗格汉斯细胞及其他:皮肤中表达朗格林的树突状细胞亚群。  |  Romani, N., et al. 2010. Immunol Rev. 234: 120-41. PMID: 20193016
  8. 靶向人类朗格林可促进HIV-1特异性体液免疫反应。  |  Kervevan, J., et al. 2021. PLoS Pathog. 17: e1009749. PMID: 34324611
  9. 小鼠特应性皮炎模型中,皮肤朗格林+树突状细胞群可促进炎症。  |  Xiao, C., et al. 2022. Front Immunol. 13: 981819. PMID: 36304463
  10. 与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779
  11. RGS 家族成员:GTPase-activating proteins for heterotrimeric G-protein alpha-subunits.  |  Watson, N., et al. 1996. Nature. 383: 172-5. PMID: 8774882
  12. RGS10 是 G alpha i GTPase 活性的选择性激活剂。  |  Hunt, TW., et al. 1996. Nature. 383: 175-7. PMID: 8774883

订购信息

产品名称产品编号规格价格数量收藏夹

RGS10 抗体 (A-8)

sc-46679
200 µg/ml
RMB2377.00

RGS10 (A-8): m-IgG Fc BP-HRP 套装

sc-528498
200 µg Ab; 10 µg BP
RMB2662.00

RGS10 (A-8): m-IgGκ BP-HRP 套装

sc-520890
200 µg Ab, 40 µg BP
RMB2662.00

RGS10 抗体 (A-8) AC

sc-46679 AC
500 µg/ml, 25% agarose
RMB3129.00

RGS10 抗体 (A-8) HRP

sc-46679 HRP
200 µg/ml
RMB2377.00

RGS10 抗体 (A-8) FITC

sc-46679 FITC
200 µg/ml
RMB2482.00

RGS10 抗体 (A-8) PE

sc-46679 PE
200 µg/ml
RMB2580.00

RGS10 抗体 (A-8) Alexa Fluor® 488

sc-46679 AF488
200 µg/ml
RMB2685.00

RGS10 抗体 (A-8) Alexa Fluor® 546

sc-46679 AF546
200 µg/ml
RMB2685.00

RGS10 抗体 (A-8) Alexa Fluor® 594

sc-46679 AF594
200 µg/ml
RMB2685.00

RGS10 抗体 (A-8) Alexa Fluor® 647

sc-46679 AF647
200 µg/ml
RMB2685.00

RGS10 抗体 (A-8) Alexa Fluor® 680

sc-46679 AF680
200 µg/ml
RMB2685.00

RGS10 抗体 (A-8) Alexa Fluor® 790

sc-46679 AF790
200 µg/ml
RMB2685.00

Has RGS10 A-8 antibody been tried against mouse or human RGS10? Do you have any other recommendations for a C-20 substitute for immunofluoresence with mouse or rat tissue? We were very dependent on the C-20 antibody, any help you can give us in replacing

Asked by: Shelley
Thank you for your question. Unfortunately we have not tested this antibody on mouse or rat tissues, so we do not know if it is reactive. If you would like to try a sample of this antibody, please contact our Technical Service Department by phone, e-mail or live chat directly on our website, and we will be happy to let you try a sample of sc-46679.
Answered by: Tech Service
Date published: 2017-10-03

I am using RGS10 (A-8): sc-46679 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from I used this in IF and works beautifully inI used this in IF and works beautifully in human cells.
Date published: 2015-10-05
Rated 5 out of 5 by from IP and WB publishable data with mouse sampleIP and WB publishable data with mouse sample - SCBT Publication Review
Date published: 2015-04-16
Rated 4 out of 5 by from Positive detection of endogenously expressedPositive detection of endogenously expressed RGS10 protein by WB using BJAB whole celll lysate and CCRF-CEM nuclear extract. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2013-11-12
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RGS10 抗体 (A-8) is rated 4.8 out of 5 by 4.
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