Date published: 2025-9-6
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RAGE Antibody (A11) - Western Blotting - Image 353151
RAGE 抗体 (A11): sc-80652. OVCAR-3 (A), HUV-EC-C (B), A549 (C) 和 Jurkat (D) 全细胞裂解液中 RAGE 表达的 Western 印迹分析.
RAGE 抗体 (A11): sc-80652. OVCAR-3 (A), HUV-EC-C (B), A549 (C) 和 Jurkat (D) 全细胞裂解液中 RAGE 表达的 Western 印迹分析.

RAGE 抗体 (A11): sc-80652

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说明书
  • RAGE抗体(A11)是小鼠单克隆IgG2a κ, 在30篇文献中引用，规格为200 µg/ml
  • 针对RAGE的human起源的截短细胞外结构域
  • 抗-RAGE 抗体 (A11) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 natural and recombinant RAGE; 也和以下物种反应，包括： bovine
  • 抗RAGE抗体（A11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RAGE (A11): sc-80652的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 RAGE 抗体 (A11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RAGE 抗体 (A11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

RAGE抗体（A11）是一种小鼠单克隆IgG2a kappa kappa轻链抗体，专门针对人类晚期糖基化终产物受体（RAGE）的截短胞外结构域。RAGE是一种I型膜蛋白，具有独特的结构，包括胞外区的一个V型免疫球蛋白结构域和两个C型免疫球蛋白结构域、一个跨膜结构域和一个短的胞浆尾部。这种结构配置有助于各种配体的结合，包括晚期糖基化终产物（AGEs）、S100蛋白和高迁移率组蛋白盒1（HMGB1），使RAGE能够启动和传播各种细胞内信号通路。RAGE的细胞外结构域对于配体的识别和结合至关重要，可触发下游效应，如NF-κB的激活和活性氧的产生，最终影响炎症反应、细胞增殖和凋亡。RAGE的精确结构特征还使其能够与其他膜蛋白形成二聚体并相互作用，从而增强RAGE在细胞通讯和信号转导中的作用。抗RAGE抗体（A11）经过优化，可用于检测小鼠、大鼠和人类来源的天然和重组RAGE，可用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。此外，RAGE单克隆抗体（A11）对牛等其他物种也有反应，从而拓宽了其在各种研究环境中的用途。通过靶向细胞外结构域，抗RAGE抗体（A11）可有效促进对RAGE的结构-功能关系及其在糖尿病、血管疾病和淀粉样变性等病理条件中的参与的研究。RAGE (A11)抗体是研究人员不可或缺的工具，有助于阐明RAGE在疾病进展中的机制作用，并开发针对RAGE介导途径的潜在治疗干预措施。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

RAGE 抗体 (A11)

sc-80652
200 µg/ml
RMB2377.00

RAGE (A11): m-IgG Fc BP-HRP 套装

sc-528697
200 µg Ab; 10 µg BP
RMB2662.00

RAGE (A11): m-IgGκ BP-HRP 套装

sc-521158
200 µg Ab, 40 µg BP
RMB2662.00

RAGE (A11): m-IgG2a BP-HRP 套装

sc-547152
200 µg Ab; 10 µg BP
RMB2662.00

RAGE 抗体 (A11) AC

sc-80652 AC
500 µg/ml, 25% agarose
RMB3129.00

RAGE 抗体 (A11) HRP

sc-80652 HRP
200 µg/ml
RMB2377.00

RAGE 抗体 (A11) FITC

sc-80652 FITC
200 µg/ml
RMB2482.00

RAGE 抗体 (A11) PE

sc-80652 PE
200 µg/ml
RMB2580.00

RAGE 抗体 (A11) Alexa Fluor® 488

sc-80652 AF488
200 µg/ml
RMB2685.00

RAGE 抗体 (A11) Alexa Fluor® 546

sc-80652 AF546
200 µg/ml
RMB2685.00

RAGE 抗体 (A11) Alexa Fluor® 594

sc-80652 AF594
200 µg/ml
RMB2685.00

RAGE 抗体 (A11) Alexa Fluor® 647

sc-80652 AF647
200 µg/ml
RMB2685.00

RAGE 抗体 (A11) Alexa Fluor® 680

sc-80652 AF680
200 µg/ml
RMB2685.00

RAGE 抗体 (A11) Alexa Fluor® 790

sc-80652 AF790
200 µg/ml
RMB2685.00

Can you please provide the amino acid sequence for the A11 Rage antibody. It is extracellular however we would like to know which AA it is associated with SC-80652. We also use the A9 rage which we are aware of the AA sequence. comparing the outcomes.

Asked by: DomMac
Thank you for your question. The amino acid sequence is proprietary but here is a link for more information. https://www.uniprot.org/uniprotkb/Q15109/entry#function
Answered by: BlakeJ
Date published: 2025-01-08

Dear Santa Cruz, Are you able to tell me the amino acid sequence contained within the 'truncated extracellular domain' that was used as the immunogen for this antibody?

Asked by: THeP
Thank you for your question. RAGE Antibody (A11) is a mouse monoclonal IgG2a raised against a truncated extracellular domain of RAGE of human origin. According to Uniprot accession # Q15109, the extracellular domain corresponds with amino acids 23-342. The exact epitope recognized by this clone has not been determined.
Answered by: Technical Support
Date published: 2017-01-31

I would like to use RAGE Antibody (A11): sc-80652 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-80652 is not available directly conjugated, but we have an alternative RAGE antibody (sc-365154) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-30
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Rated 1 out of 5 by from non funziona in wbTestato su macrofagi umani e non ha funzionato in western blot
Date published: 2023-03-17
Rated 5 out of 5 by from specific band in WBIt's hard to find a good quality antibody, this antibody detects RAGE protein at 1:800 dilution in WB assays . Works well in mouse kidney tissue , always works in every condition. Easy to use.
Date published: 2017-07-29
Rated 5 out of 5 by from excellent antibodyUsed with very good results in human colon tissue with IHC and cells
Date published: 2017-05-16
Rated 5 out of 5 by from strong antibodyBrought up clear bands at the predicted molecular weight on western blots
Date published: 2017-04-17
Rated 5 out of 5 by from Well characterized RAGE ab for WB, FCM & IHCThis antibody is well published in a variety of human tissue samples. Strong WB banding seen in OVCAR-3, HUV-EC-C, A549 and Jurkat whole cell lysates. and beautiful Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue.
Date published: 2017-01-06
Rated 4 out of 5 by from Various publishable data in WB using humanVarious publishable data in WB using human osteosarcoma cell lines KPDX, lung tissue and HUVECs. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Great band seen in WB using IMRGreat band seen in WB using IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-05-09
Rated 4 out of 5 by from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-02-20
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RAGE 抗体 (A11) is rated 4.2 out of 5 by 9.
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