Date published: 2025-9-6
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RAGE Antibody (A-9) - Western Blotting - Image 385727
RAGE 抗体 (A-9) HRP: sc-365154 HRP. 直接 Western 印迹分析 A549 (A), OVCAR-3 (B), ECV304 (C), HeLa (D), IMR-32 (E) 和 MCF7 (F) 全细胞裂解液中 RAGE 的表达.
RAGE 抗体 (A-9) HRP: sc-365154 HRP. 直接 Western 印迹分析 A549 (A), OVCAR-3 (B), ECV304 (C), HeLa (D), IMR-32 (E) 和 MCF7 (F) 全细胞裂解液中 RAGE 的表达.

RAGE 抗体 (A-9): sc-365154

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说明书
  • RAGE 抗体 A-9 是小鼠单克隆 IgG1 κ，RAGE抗体, 在128篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的RAGE的N-terminus的氨基酸23-43之间
  • RAGE 抗体 (A-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 RAGE
  • 抗RAGE抗体（A-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RAGE (A-9): sc-365154的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 RAGE 抗体 (A-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RAGE 抗体 (A-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

RAGE抗体（A-9）是一种IgG1κ小鼠单克隆RAGE抗体（也称为晚期糖基化终产物特异性受体抗体、AGER抗体或SCARJ1抗体），它通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的RAGE蛋白。RAGE抗体（A-9）既有非偶联的抗RAGE抗体形式，也有多种偶联形式的抗RAGE抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。蛋白质的高级糖基化终末产物（AGEs）是与多种疾病相关的非酶糖基化蛋白质，包括糖尿病和其他血管疾病，以及淀粉样变性。这些蛋白质通过特定的细胞表面受体分子（如RAGE，即晚期糖基化终末产物受体）调节细胞功能。RAGE是一种1型膜蛋白，存在于内皮细胞、单核吞噬细胞和血管平滑肌细胞的表面。AGEs与RAGE结合会导致细胞氧化应激的诱导和转录因子NFkB的激活。有证据表明，氧化应激的诱导会导致涉及p21 ras和MAP激酶的细胞内级联反应的激活，从而引发转录的激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RAGE 抗体 (A-9) 参考文献:

  1. 蛋白质晚期糖基化终产物细胞表面受体的克隆与表达。  |  Neeper, M., et al. 1992. J Biol Chem. 267: 14998-5004. PMID: 1378843
  2. 高级糖化终产物与其受体/结合蛋白的相互作用增强了细胞氧化应激。  |  Yan, SD., et al. 1994. J Biol Chem. 269: 9889-97. PMID: 8144582
  3. RAGE：高级糖化终产物的新型细胞受体。  |  Schmidt, AM., et al. 1996. Diabetes. 45 Suppl 3: S77-80. PMID: 8674899
  4. 高级糖化终产物受体（RAGE）是 AGE-β2微球蛋白通过氧化剂敏感途径与人类单核吞噬细胞相互作用的核心介质。对透析相关淀粉样变性发病机制的影响。  |  Miyata, T., et al. 1996. J Clin Invest. 98: 1088-94. PMID: 8787669
  5. 高级糖化终产物, 氧化应激和血管病变。  |  Chappey, O., et al. 1997. Eur J Clin Invest. 27: 97-108. PMID: 9061302
  6. 高级糖化终产物受体 RAGE 启动子的特征和功能分析。  |  Li, J. and Schmidt, AM. 1997. J Biol Chem. 272: 16498-506. PMID: 9195959
  7. 晚期糖化终产物受体的激活会触发受氧化应激调控的依赖于 p21(ras) 的丝裂原活化蛋白激酶通路。  |  Lander, HM., et al. 1997. J Biol Chem. 272: 17810-4. PMID: 9211935

订购信息

产品名称产品编号规格价格数量收藏夹

RAGE 抗体 (A-9)

sc-365154
200 µg/ml
RMB2377.00

RAGE (A-9): m-IgG Fc BP-HRP 套装

sc-529316
200 µg Ab; 10 µg BP
RMB2662.00

RAGE (A-9): m-IgGκ BP-HRP 套装

sc-522147
200 µg Ab, 40 µg BP
RMB2662.00

RAGE (A-9): m-IgG1 BP-HRP 套装

sc-543451
200 µg Ab; 20 µg BP
RMB2662.00

RAGE 抗体 (A-9) AC

sc-365154 AC
500 µg/ml, 25% agarose
RMB3129.00

RAGE 抗体 (A-9) HRP

sc-365154 HRP
200 µg/ml
RMB2377.00

RAGE 抗体 (A-9) FITC

sc-365154 FITC
200 µg/ml
RMB2482.00

RAGE 抗体 (A-9) PE

sc-365154 PE
200 µg/ml
RMB2580.00

RAGE 抗体 (A-9) Alexa Fluor® 488

sc-365154 AF488
200 µg/ml
RMB2685.00

RAGE 抗体 (A-9) Alexa Fluor® 546

sc-365154 AF546
200 µg/ml
RMB2685.00

RAGE 抗体 (A-9) Alexa Fluor® 594

sc-365154 AF594
200 µg/ml
RMB2685.00

RAGE 抗体 (A-9) Alexa Fluor® 647

sc-365154 AF647
200 µg/ml
RMB2685.00

RAGE 抗体 (A-9) Alexa Fluor® 680

sc-365154 AF680
200 µg/ml
RMB2685.00

RAGE 抗体 (A-9) Alexa Fluor® 790

sc-365154 AF790
200 µg/ml
RMB2685.00

RAGE (A-9) 中和勝肽

sc-365154 P
100 µg/0.5 ml
RMB511.00

I would lie to ask you what clone is this antibody: RAGE Antibody (A-9): sc-365154.

Asked by: Kamila
Thank you for your question, this antibody's clone name is A-9.
Answered by: Tech Support Europe
Date published: 2024-11-14

RAGE is a membrane protein. Why the whole cells were stained with this antibody (Anti-RAGE Antibody (A-9), sc-365154) for immunofluorescence in my experiment?

Asked by: Harrypotter
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 12
Date published: 2021-11-23

What is the difference between the Anti-RAGE Antibody (A-9), sc-365154, 200 µg/ml and Anti-RAGE Antibody (A-9): m-IgG Fc BP-HRP Bundle, sc-529316, 200 µg Ab, 10 µg BP? Do the latter include the Secondary Antibody as well?

Asked by: Puspanjali
Thank you for your inquiry. The bundle contains the appropriate secondary binding protein for HRP-based detection of the primary Anti-RAGE Antibody.
Answered by: Tech Support Europe
Date published: 2021-09-23

which dilution should I use for WB? did you try it with Hela cells? Thanks!!

Asked by: CAJ01
Thank you for your question. We recommend a starting dilution of 1:100 for this antibody for Western Blot. You can see Western blot results with Hela cells above in our published data.
Answered by: Tech Service
Date published: 2019-08-27

On the figure showing A9 detection of RAGE in various cell types, how much protein was loaded per lane? Also, does this antibody pick up mouse RAGE in Western blots? thanks.

Asked by: Stefanie Vogel
Thank you for your question. We loaded 50 ug whole cell lysate protein. Yes, this antibody is recommended for detection of RAGE of mouse, rat and human origin.
Answered by: Technical Support
Date published: 2019-05-25

This antibody is noted to be able to be used for FCM. Which concentration of antibody is used for this? I couldn't find it in the protocol sheet. Thanks!

Asked by: ktiv
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates! We recommend using approximately 1 ug of the conjugated antibody per 1 million cells. Below is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2019-02-06

Does this monoclonal antibody cross the blood brain barrier?

Asked by: vic6
Thank you for your question. This antibody is for research use only, not for in vivo use. I do not have any information regarding antibody passage across the blood brain barrier. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-26

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-365154 PE), fluorescein (sc-365154 FITC), Alexa Fluor® 488 (sc-365154 AF488) or Alexa Fluor® 647 (sc-365154 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-04
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Rated 5 out of 5 by from funziona benissimo in wbTestato su macrofagi umani e ha funzionato in western blot
Date published: 2023-03-17
Rated 2 out of 5 by from Low signal in PBMC lysatesI used this antibody for WB 1:200 concentration in PBMC and the signal was very low
Date published: 2020-10-14
Rated 5 out of 5 by from 功能不错，效果不错之前听同学说买了效果不错，尝试一下，看看能不能做的出来，我的是小鼠脑组织，希望不要存在太多的非特异性条带。
Date published: 2019-03-10
Rated 4 out of 5 by from Good antibody, strong signalWorks well with human neutrophils and lung epithelial cells
Date published: 2018-05-24
Rated 5 out of 5 by from RAGE antibodyWe tried this antibody for mouse heart tissue. Clear bands appeared. Really nice antibody!
Date published: 2018-02-22
Rated 5 out of 5 by from Good performanceUse the normal wb Protocol ,blocking 4℃ overnight use 5% skim milk,the band is very specific at 46kda with no background,loading 40ug mouse colon tissue lysate.
Date published: 2018-01-27
Rated 5 out of 5 by from nice antibodythe mouse antibody was good to use, thanks a lot,
Date published: 2017-10-11
Rated 5 out of 5 by from this trial pack is good for use。this trial pack is good for use。I use it for immunohistochemistry。My experimental sample comes from the mouse's joints。Positive cells can be seen。
Date published: 2017-09-20
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RAGE 抗体 (A-9) is rated 4.5 out of 5 by 32.
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