Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Rad51 Antibody (F-11) - Western Blotting - Image 360058
Rad51 抗体 (F-11) HRP: sc-398587 HRP. K-562 (A), A-431 (B), HeLa (C), Ramos (D) 和 NIH/3T3 (E) 全细胞裂解液中 Rad51 表达的直接 Western 印迹分析.
Rad51 抗体 (F-11) HRP: sc-398587 HRP. K-562 (A), A-431 (B), HeLa (C), Ramos (D) 和 NIH/3T3 (E) 全细胞裂解液中 Rad51 表达的直接 Western 印迹分析.

Rad51 抗体 (F-11): sc-398587

3.2(19)
寫評論提問

说明书
  • Rad51 抗体 F-11 是小鼠单克隆 IgG1 κ，Rad51抗体, 在39篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Rad51的C-terminus的氨基酸311-339之间
  • Rad51 抗体 (F-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Rad51
  • 抗Rad51抗体（F-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rad51 (F-11): sc-398587的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Rad51 抗体 (F-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Rad51 抗体 (F-11) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Rad 抗体 包括 Rad1, Rad17, Rad18, Rad21, Rad23A, Rad23B, Rad26L, Rad50, Rad51和Rad51B

Rad51抗体（F-11）是一种IgG1 κ小鼠单克隆Rad51抗体，可通过WB、IP、IF、IHC(P)和ELISA方法检测来自小鼠、大鼠和人类的Rad51蛋白。Rad51抗体（F-11）既有非偶联的抗Rad51抗体形式，也有多种偶联形式的抗Rad51抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Rad52家族成员（Rad50、Rad51B/C/D、Rad52、Rad54、MRE11）介导DNA双链断裂修复（DSBR），以修复可能导致细胞死亡、突变或肿瘤转化的DNA损伤。Rad51（RECA，BRCC5）与BRCA1和BRCA2相互作用，影响亚细胞定位和细胞对DNA损伤的反应。BRCA2的失活可能是导致基因组不稳定和Rad51失调引发肿瘤发生的关键事件。Rad52形成一个七聚体环，该环与单链DNA末端结合，并催化互补链退火所需的DNA-DNA相互作用。Rad52可以与Rad51相互作用。DEAD样解旋酶超家族的Rad54A与双链DNA结合并诱导DNA拓扑结构变化，这被认为有助于同源DNA配对并刺激DNA重组。DEAD样解旋酶超家族的Rad54B与双链DNA结合，并在DNA存在下显示ATP酶活性。Rad54B在睾丸和脾脏中含量丰富，该基因的突变发生在原发性淋巴瘤和结肠癌中。MRE11（减数分裂重组11，ATLD，HNGS1）是一种核3'-5'核酸外切酶/内切酶，与Rad50相关，影响同源重组、端粒长度维持和DNA双链断裂修复。MRE11在增殖组织中含量最为丰富。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Rad51 抗体 (F-11)

sc-398587
200 µg/ml
RMB2377.00

Rad51 (F-11): m-IgG Fc BP-HRP 套装

sc-530857
200 µg Ab; 10 µg BP
RMB2662.00

Rad51 (F-11): m-IgGκ BP-HRP 套装

sc-524406
200 µg Ab, 40 µg BP
RMB2662.00

Rad51 (F-11): m-IgG1 BP-HRP 套装

sc-544282
200 µg Ab; 20 µg BP
RMB2662.00

Rad51 抗体 (F-11) AC

sc-398587 AC
500 µg/ml, 25% agarose
RMB3129.00

Rad51 抗体 (F-11) HRP

sc-398587 HRP
200 µg/ml
RMB2377.00

Rad51 抗体 (F-11) FITC

sc-398587 FITC
200 µg/ml
RMB2482.00

Rad51 抗体 (F-11) PE

sc-398587 PE
200 µg/ml
RMB2580.00

Rad51 抗体 (F-11) Alexa Fluor® 488

sc-398587 AF488
200 µg/ml
RMB2685.00

Rad51 抗体 (F-11) Alexa Fluor® 546

sc-398587 AF546
200 µg/ml
RMB2685.00

Rad51 抗体 (F-11) Alexa Fluor® 594

sc-398587 AF594
200 µg/ml
RMB2685.00

Rad51 抗体 (F-11) Alexa Fluor® 647

sc-398587 AF647
200 µg/ml
RMB2685.00

Rad51 抗体 (F-11) Alexa Fluor® 680

sc-398587 AF680
200 µg/ml
RMB2685.00

Rad51 抗体 (F-11) Alexa Fluor® 790

sc-398587 AF790
200 µg/ml
RMB2685.00

Rad51 (F-11) 中和勝肽

sc-398587 P
100 µg/0.5 ml
RMB511.00

Hello, I am thinking of trying sc-398587 Rad51 F-11 for IF in human cancer cells but I see some bad reviews for IF (and WB). Do you have protocol recommendations (as far as pre-extracting or not etc)? Thank you.

Asked by: adkjfd
Thank you for your question. sc-398587 is a great antibody for Rad51. It is recommended and warrantied for IF on human, mouse, and rat samples. Here is a recent publication using it for IF on human samples (PMID: 29694959). Here is a link to the recommended protocol. We recommend a starting dilution of 1:50. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Service
Date published: 2024-08-13

If I want to test the specificity of RAD51, can I also have a sample of the blocking peptide sc-398587 P? Please let me know. Thanks.

Asked by: harias
Thank you for your question. We do not provide sample sizes of the blocking peptides.
Answered by: Technical Support
Date published: 2019-05-25

Do you have the one which can be used for the flow cytometry?

Asked by: ning25348
Thank you for your question. Unfortunately we do not offer an antibody that has been tested for Flow cytometry against Rad51, so they can not be recommended or warrantied for that use. Please contact Technical Service if you have any further questions.
Answered by: Tech Service
Date published: 2019-01-14

How can I get a trial sample for the monoclonal Ab Rad51 (F-11): sc-398587? Please let me know.

Asked by: harias
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

What is the recommended antigen retrieval method when doing IHC(P) with this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using sodium citrate buffer, pH 6.0, and heat for antigen retrieval. The full recommended protocol can be found on our website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-30
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_398587, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from very very badno band (different size band coming) and no IF staining. very bad
Date published: 2018-09-05
Rated 1 out of 5 by from Didn't work in my hands.Tried for western at 1:500 dilution and didn't detect any band in human neuroblastoma cancer cells. The discontinued works perfectly fine in the same lysates.
Date published: 2018-06-26
Rated 1 out of 5 by from Tried twice for WB, just got a smeary mess.Tried this as a sample, very disappointed. I firstly tried 1:100 5% milk in TBST, incubated for 1.5 hours at room temperature. I got nothing but smears on the blot. I then tried 1:500 incubation for 1.5 hours at room temperature followed by overnight incubation at 4 oC. This is the blot shown in he picture. Nothing at 37kDa and lots of messy smears, does not work at all. Loaded 30ug protein per well with llamaeli buffer. Normally works very well. GAPDH control (not shown) also worked well.
Date published: 2018-01-25
Rated 5 out of 5 by from 这个抗体和之前一样好用！多年前买过santa cruz抗体，质量很好，物美价廉。这次试用的抗体一如既往的好用，以后会继续关注santa cruz。
Date published: 2017-12-19
Rated 5 out of 5 by from Good antibodyI tried this antibody for Rad51. It is very good! The blot is very clean and clear. The cell line is U2OS. The middle lane is from cells infected by Rad51 shRNA.
Date published: 2017-11-04
Rated 3 out of 5 by from can't recognize any band in NPC cell lysatescan't recognize any band in NPC cell lysates，1：250 in 5%TBST,4℃ overnight
Date published: 2017-09-22
Rated 5 out of 5 by from Great for WBWorks great for WB at 1:1000 dilution in HeLa and 293T cells.
Date published: 2017-09-06
Rated 1 out of 5 by from It is not good for IF.Tried this antibody, but there was no signal at 1:50 diluted.
Date published: 2017-08-02
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_zh_CN, sid_398587, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
Rad51 抗体 (F-11) is rated 3.2 out of 5 by 19.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_zh_CN, sid_398587, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 112ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。