Date published: 2025-9-7
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PUMAα/β Antibody (G-3) - Western Blotting - Image 311931
PUMAα/β 抗体 (G-3): sc-374223. A549 (A), Hep G2 (B), HeLa (C) 和 K-562 (D) 全细胞裂解液中 PUMAα/β 表达的 Western 印迹分析.
PUMAα/β 抗体 (G-3): sc-374223. A549 (A), Hep G2 (B), HeLa (C) 和 K-562 (D) 全细胞裂解液中 PUMAα/β 表达的 Western 印迹分析.

PUMAα/β 抗体 (G-3): sc-374223

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说明书
  • PUMA alpha / beta 抗体 G-3 是小鼠单克隆 IgG2b κ，PUMAα/β抗体, 在74篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列57-193位于human物种的PUMAα的C-terminus
  • PUMA alpha / beta 抗体 (G-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 PUMAα and PUMAβ
  • 抗PUMA alpha / beta抗体（G-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PUMAα/β (G-3): sc-374223的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成PUMAα/β 抗体 (G-3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PUMA 抗体包括 PUMA α, PUMA β 和 PUMA δ

PUMAα/β抗体（G-3）是一种IgG2b κ小鼠单克隆PUMAα/β抗体（也被称为PUMA抗体、BBC3抗体或JFY1抗体），通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的PUMAα/β蛋白。PUMAα/β抗体（G-3）既可作为非偶联抗PUMAα/β抗体形式提供，也可作为多种偶联形式的抗PUMAα/β抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。PUMA（Bcl-2结合组分3、JFY1、PUMA/JFY1）是一种线粒体促凋亡Bcl-2同源结构域（BH3）蛋白，仅通过Bax和线粒体依赖途径诱导快速凋亡。PUMA基因编码四种蛋白质，这些蛋白质来自同一转录本的不同剪接变异体：PUMAα、β、γ和δ。PUMAα和PUMAβ都包含一个BH3结构域，而PUMAγ和PUMAδ则缺乏该结构域。BH3结构域对于PUMAα和PUMAβ与Bcl-2或Bcl-xL的结合至关重要。PUMA是体内淋巴细胞中γ辐射凋亡和糖皮质激素诱导凋亡的启动子。Bcl-2家族成员通常调节凋亡并将死亡信号传递给线粒体。该家族的成员包括促进凋亡和抗凋亡的蛋白质，它们共享被称为Bcl-2同源结构域（BH1-4）的同源序列。BH3蛋白BID、NOXA、PUMA、NBK、Bim和Bad都具有促凋亡作用，并在两亲性α-螺旋BH3区域内共享序列同源性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PUMAα/β 抗体 (G-3) 参考文献:

  1. PUMA 可诱导结直肠癌细胞快速凋亡。  |  Yu, J., et al. 2001. Mol Cell. 7: 673-82. PMID: 11463391
  2. PUMA是一种新型促凋亡基因，由p53诱导。  |  Nakano, K. and Vousden, KH. 2001. Mol Cell. 7: 683-94. PMID: 11463392
  3. bbc3 是一种促凋亡的纯 BH3 基因，其表达受多种细胞死亡和存活信号的调控。  |  Han, J., et al. 2001. Proc Natl Acad Sci U S A. 98: 11318-23. PMID: 11572983
  4. 纯 BH3 蛋白--进化保守的促凋亡 Bcl-2 家族成员，对启动程序性细胞死亡至关重要。  |  Bouillet, P. and Strasser, A. 2002. J Cell Sci. 115: 1567-74. PMID: 11950875
  5. Puma 是依赖 p53 和不依赖 p53 的细胞凋亡途径的重要介质。  |  Jeffers, JR., et al. 2003. Cancer Cell. 4: 321-8. PMID: 14585359
  6. p53 靶标 PUMA 抑制肿瘤发生。  |  Hemann, MT., et al. 2004. Proc Natl Acad Sci U S A. 101: 9333-8. PMID: 15192153
  7. p53 激活域 1 对于 PUMA 上调和 p53 介导的神经元细胞死亡至关重要。  |  Cregan, SP., et al. 2004. J Neurosci. 24: 10003-12. PMID: 15525786
  8. Bax 的第一个 alpha 螺旋在配体诱导的 Bid 和 PUMA 激活过程中发挥了必要的作用。  |  Cartron, PF., et al. 2004. Mol Cell. 16: 807-18. PMID: 15574335
  9. 纯 BH3 蛋白 Puma 和 Bim 是伽马射线和糖皮质激素诱导体内淋巴细胞凋亡的限速因子。  |  Erlacher, M., et al. 2005. Blood. 106: 4131-8. PMID: 16118324
  10. PUMA-alpha的诱导和PUMA-beta的下调与苯并（a）芘诱导的MCF-7细胞凋亡有关。  |  Tampio, M., et al. 2009. Toxicol Lett. 188: 214-22. PMID: 19397966
  11. PUMA 促进 EMI1 推动的细胞质 Rad51 泛素化，并抑制干细胞和祖细胞的 DNA 修复。  |  Kang, JW., et al. 2021. Signal Transduct Target Ther. 6: 129. PMID: 33785736
  12. PUMA的羧基末端序列与抗凋亡蛋白和膜结合。  |  Pemberton, JM., et al. 2023. Elife. 12: PMID: 37078707

订购信息

产品名称产品编号规格价格数量收藏夹

PUMAα/β 抗体 (G-3)

sc-374223
200 µg/ml
RMB2377.00

PUMAα/β 抗体 (G-3) AC

sc-374223 AC
500 µg/ml, 25% agarose
RMB3129.00

PUMAα/β 抗体 (G-3) HRP

sc-374223 HRP
200 µg/ml
RMB2377.00

PUMAα/β 抗体 (G-3) FITC

sc-374223 FITC
200 µg/ml
RMB2482.00

PUMAα/β 抗体 (G-3) PE

sc-374223 PE
200 µg/ml
RMB2580.00

PUMAα/β 抗体 (G-3) Alexa Fluor® 488

sc-374223 AF488
200 µg/ml
RMB2685.00

PUMAα/β 抗体 (G-3) Alexa Fluor® 546

sc-374223 AF546
200 µg/ml
RMB2685.00

PUMAα/β 抗体 (G-3) Alexa Fluor® 594

sc-374223 AF594
200 µg/ml
RMB2685.00

PUMAα/β 抗体 (G-3) Alexa Fluor® 647

sc-374223 AF647
200 µg/ml
RMB2685.00

PUMAα/β 抗体 (G-3) Alexa Fluor® 680

sc-374223 AF680
200 µg/ml
RMB2685.00

PUMAα/β 抗体 (G-3) Alexa Fluor® 790

sc-374223 AF790
200 µg/ml
RMB2685.00

What is the quantity (volume) per unit? the ordering information only describes the product concentration.

Asked by: dalejandra
Thank you for your question. This product is supplied as 200 µg in 1 ml PBS with gelatin and sodium azide. You can find this and additional information in the antibody's datasheet: https://datasheets.scbt.com/sc-374223.pdf
Answered by: Tech Support Europe
Date published: 2022-10-25

Is this antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: cjMara
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected]. Alternatively, consider using PUMAα (B-6): sc-377015 for PUMAα/β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. (https://www.scbt.com/scbt/product/pumaalpha-antibody-b-6)
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 4 out of 5 by from awesome antibodyThe antibody is quite good. It is very classical and well-established antibody. The signal is strong and the specificity is very high. High recommended!
Date published: 2024-05-13
Rated 1 out of 5 by from Non funzionaAbbiamo testato l'anticorpo su 4 linee cellulari differenti e a diverse concentrazioni: 1:500, 1:200 e 1:100, ma l'anticorpo non riconosce l'epitopo e non riusciamo a visualizzare nessuna banda
Date published: 2020-09-18
Rated 3 out of 5 by from Work with western blotThe antibody can detect a specific band with 1:250 dilution for western blot, but signal is weaker. Samples were HeLa cell extract.
Date published: 2019-10-08
Rated 4 out of 5 by from good antibodydetects bands at the expected size. inducibility upon an apoptotic stimulus could be demonstrated. also background signals.
Date published: 2019-06-21
Rated 5 out of 5 by from good resultsIt worked well for western blotting in hepatocellular cancer cells.
Date published: 2019-03-05
Rated 5 out of 5 by from nice signalused 1:1.000 in HUVEC cells, nice signal at expected MW, no unspecific.
Date published: 2018-11-08
Rated 4 out of 5 by from Good staining in westernDuplicated the results of the PUMA mAb from cell signaling, but slightly elevated background. Verified it in lines where I knockdown PUMA.
Date published: 2018-04-23
Rated 5 out of 5 by from Used this antibody for ICCIt was used in U2OS cells with KO for apoptosis signaling and clearly shows good signals and colocalizes with mitotracker red confirming mitochondrial localization. Used at 1:300 dilution
Date published: 2018-04-12
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PUMAα/β 抗体 (G-3) is rated 4.6 out of 5 by 27.
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