Date published: 2025-9-8
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PU.1/Spi1 Antibody (C-3) - Western Blotting - Image 384033
PU. 1 抗体 (C-3) HRP: sc-390405 HRP. MM-142 (A) 和 THP-1 (C) 全细胞裂解液及 RAW 264. 7 核提取物 (B) 中 PU. 1 表达的直接 Western 印迹分析.
PU. 1 抗体 (C-3) HRP: sc-390405 HRP. MM-142 (A) 和 THP-1 (C) 全细胞裂解液及 RAW 264. 7 核提取物 (B) 中 PU. 1 表达的直接 Western 印迹分析.

PU.1/Spi1 抗体 (C-3): sc-390405

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寫評論提問

说明书
  • PU.1/Spi1 抗体 C-3 是小鼠单克隆 IgG1 κ，PU.1/Spi1抗体, 在23篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的PU.1的N-terminus附近的氨基酸25-63之间
  • PU.1/Spi1 抗体 (C-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PU.1
  • 抗PU.1/Spi1抗体（C-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-390405 X, 200 µg/0.1 ml)
  • 关于如何获取PU.1/Spi1 (C-3): sc-390405的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 PU.1/Spi1 抗体 (C-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PU.1/Spi1 抗体 (C-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

PU.1抗体（C-3）是一种IgG1κ小鼠单克隆PU.1抗体（也称为Spi-1原癌基因抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的PU.1蛋白。PU.1抗体（C-3）既可作为非缀合的抗PU.1抗体形式，也可作为多种缀合形式的抗PU.1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®缀合物。Ets转录因子家族（Ets-1、Ets-2、Erg-1-3、Elk-1、Elf-1、Elf-5、NERF、PU.1、PEA3、ERM、FEV、ER8l、Fli-1、TEL、Spi-B、ESE-1、ESE-3A、Net、ABT1和ERF）是影响淋巴发育和活动的DNA结合蛋白。Ets家族的单体蛋白通过一种独特的带翼螺旋-转角-螺旋基序（称为Ets结构域）与共识DNA位点GGA(A/T)结合。PU.1（Spi-1/Spi-A）、Spi-B和Spi-C是密切相关的Ets家族成员，它们在Ets结构域内共享一个保守的发散序列，使其能够与非规范的AGAA位点结合。PU.1反式激活大量B细胞基因，如编码CD72、CD20和Btk的基因，而Spi-B则增强许多这些相同靶基因的表达。PU.1在多种造血细胞中表达，包括B细胞、早期T细胞、巨核细胞、粒细胞、肥大细胞、未成熟红细胞和髓样细胞。另外，Spi-B的表达仅限于B细胞和未成熟的T细胞，在这些细胞中，表达通过T细胞谱系承诺而累积，然后在β选择检查点之后显著缺失。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PU.1/Spi1 抗体 (C-3) 参考文献:

  1. Spi-C，一种在 B 淋巴细胞发育过程中受时间调控的新型 Ets 蛋白。  |  Bemark, M., et al. 1999. J Biol Chem. 274: 10259-67. PMID: 10187812
  2. 正常的 B 细胞受体介导的信号转导需要 PU.1 和 Spi-B。  |  Garrett-Sinha, LA., et al. 1999. Immunity. 10: 399-408. PMID: 10229183
  3. Ets家族转录因子在T细胞系的规格化和承诺过程中的精确发育调控。  |  Anderson, MK., et al. 1999. Development. 126: 3131-48. PMID: 10375504
  4. Ets因子PU.1和Spi-B调节淋巴受限七螺旋受体P2Y10的体内转录。  |  Rao, S., et al. 1999. J Biol Chem. 274: 34245-52. PMID: 10567398
  5. 转录因子PU.1和免疫细胞分化（综述）。  |  Li, G., et al. 2020. Int J Mol Med. 46: 1943-1950. PMID: 33125129
  6. 关键转录因子PU.1在自身免疫性疾病中的作用。  |  Fang, Y., et al. 2022. Front Immunol. 13: 1001201. PMID: 36248862
  7. PU.1是一种有用的核标记，可用于区分组织细胞增生症和富含组织细胞的肿瘤。  |  Ungureanu, IA., et al. 2023. Histopathology. 83: 320-325. PMID: 37012662
  8. PU.1 (Spi-1)能自动调节其在骨髓细胞中的表达。  |  Chen, H., et al. 1995. Oncogene. 11: 1549-60. PMID: 7478579
  9. Ets1 转录因子在小鼠胚胎发育过程中广泛表达，与参与器官形成等形态发生过程的中胚层细胞有关。  |  Kola, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 7588-92. PMID: 7689222
  10. 中性粒细胞和单核细胞表达高水平的 PU.1 (Spi-1)，但不表达 Spi-B。  |  Chen, HM., et al. 1995. Blood. 85: 2918-28. PMID: 7742552
  11. 在发育过程中，Ets 蛋白 Spi-B 只在 B 细胞和 T 细胞中表达。  |  Su, GH., et al. 1996. J Exp Med. 184: 203-14. PMID: 8691135
  12. PU.1：造血和白血病中的重要多面手。  |  Kastner, P. and Chan, S. 2008. Int J Biochem Cell Biol. 40: 22-7. PMID: 17374502

订购信息

产品名称产品编号规格价格数量收藏夹

PU.1/Spi1 抗体 (C-3)

sc-390405
200 µg/ml
RMB2377.00

PU.1/Spi1 (C-3): m-IgG Fc BP-HRP 套装

sc-530145
200 µg Ab; 10 µg BP
RMB2662.00

PU.1/Spi1 (C-3): m-IgGκ BP-HRP 套装

sc-523521
200 µg Ab, 40 µg BP
RMB2662.00

PU.1/Spi1 (C-3): m-IgG1 BP-HRP 套装

sc-543891
200 µg Ab; 20 µg BP
RMB2662.00

PU.1/Spi1 抗体 (C-3) X

sc-390405 X
200 µg/0.1 ml
RMB2377.00

PU.1/Spi1 抗体 (C-3) AC

sc-390405 AC
500 µg/ml, 25% agarose
RMB3129.00

PU.1/Spi1 抗体 (C-3) HRP

sc-390405 HRP
200 µg/ml
RMB2377.00

PU.1/Spi1 抗体 (C-3) FITC

sc-390405 FITC
200 µg/ml
RMB2482.00

PU.1/Spi1 抗体 (C-3) PE

sc-390405 PE
200 µg/ml
RMB2580.00

PU.1/Spi1 抗体 (C-3) Alexa Fluor® 488

sc-390405 AF488
200 µg/ml
RMB2685.00

PU.1/Spi1 抗体 (C-3) Alexa Fluor® 546

sc-390405 AF546
200 µg/ml
RMB2685.00

PU.1/Spi1 抗体 (C-3) Alexa Fluor® 594

sc-390405 AF594
200 µg/ml
RMB2685.00

PU.1/Spi1 抗体 (C-3) Alexa Fluor® 647

sc-390405 AF647
200 µg/ml
RMB2685.00

PU.1/Spi1 抗体 (C-3) Alexa Fluor® 680

sc-390405 AF680
200 µg/ml
RMB2685.00

PU.1/Spi1 抗体 (C-3) Alexa Fluor® 790

sc-390405 AF790
200 µg/ml
RMB2685.00

PU.1/Spi1 (C-3) 中和勝肽

sc-390405 P
100 µg/0.5 ml
RMB511.00

Previous publications have listed this antibody as being used for ChIP or ChIP-seq, but ChIP is not listed as an appropriate technique for this antibody, unless a certain reagent is purchased. Is there additional information on this reagent?

Asked by: jbecho
Thank you for your question. sc-390405 X is recommended for ChIP assay. Here is our protocol :https://www.scbt.com/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: BlakeJ
Date published: 2025-07-23

Hi, does this antibody work for ChIP ? Any reference paper? Thank you !

Asked by: Brice
Thank you for your question. The TransCruz reagent (sc-390405 X, 200 µg/0.1 ml) version of this primary antibody is recommended and warrantied for ChIP applications, however we are still gathering data to show on our website. Check back for updates! We are not yet aware of any product citations using this new mouse monoclonal antibody, however, there is a product citation using a different PU.1 antibody for ChIP, PU.1 Antibody (B-9): sc-390659, please refer to PMID: 29894691 for this reference.
Answered by: Technical Support
Date published: 2024-12-04

What application is the blocking peptide sc-390405 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from No clear signal in human PSC-derived cellsThe antibody signal couldn't be detected in hPSC-derived macrophage cultures by immunofluorescence (dilution 1:50).
Date published: 2023-08-01
Rated 4 out of 5 by from Good antibodyThis antibody works well in Immunoblot assay at 1:500 dilution in NB4 cell line.
Date published: 2021-01-26
Rated 1 out of 5 by from This antibody does not recognize chicken PU.1Unfortunately, this antibody does not recognize chicken PU.1
Date published: 2018-01-29
Rated 5 out of 5 by from Gutes Signal im Western BlotHaben den PU.1 C3 monoklonalen Antikörper bei einer 1:200 Verdünnung in Milchpulver/TBS-T an murinen Lymphomen, Milz, Makrophagen und iPSCs getestet. Positives, gut detektierbares Signal als Doppelbande (etwa 37/40 kDa) in allen Zelltypen außer den iPSCs bekommen. Bei einigen Zelltypen unspezifische (?) Banden (bei etwa 32 und unterhalb 25 kDa).
Date published: 2017-09-27
Rated 5 out of 5 by from High specificHigh specific antibody,I have tried different conditions ,such as the dilutions, incubated time and expose time, no problem.
Date published: 2017-06-15
Rated 5 out of 5 by from PU.1抗体验证申请一个PU.1抗体，本来没抱很大希望，但是居然很好使。我使用的是293T，RAW264.7,PAM，以及Marc-145细胞，都能显出内源性的PU.1.其中RAW细胞条带最粗，293T细胞内源性比较少。
Date published: 2017-06-14
Rated 5 out of 5 by from Great antibodyPU.1 Antibody (C-3) is a great antibody for immunofluorescent applications, showed strong staining
Date published: 2017-06-01
Rated 5 out of 5 by from Works well in WBworks as expected in Western Blotting on MM-142 whole cell lysate. Clean strong band at 1:200 dilution.
Date published: 2017-01-27
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PU.1/Spi1 抗体 (C-3) is rated 4.1 out of 5 by 10.
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