Date published: 2025-9-6
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PTP1B Antibody (D-4) - Western Blotting - Image 374409
PTP1B 抗体 (D-4): sc-133259. HL-60 (A), CCRF-HSB-2 (B) 和 Hep G2 (C) 全细胞裂解液中 PTP1B 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
PTP1B 抗体 (D-4): sc-133259. HL-60 (A), CCRF-HSB-2 (B) 和 Hep G2 (C) 全细胞裂解液中 PTP1B 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

PTP1B 抗体 (D-4): sc-133259

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寫評論提問

说明书
  • PTP1B 抗体 D-4 是小鼠单克隆 IgG1 κ，PTP1B抗体, 在16篇文献中引用，规格为200 µg/ml
  • 针对氨基酸301-435，在PTP1B (protein tyrosine phosphatase)的human起源的C-terminus处进行映射
  • PTP1B 抗体 (D-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 PTP1B
  • 抗PTP1B抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PTP1B (D-4): sc-133259的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 PTP1B 抗体 (D-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PTP1B 抗体 (D-4) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 PTP 抗体 包括 PTP IA-2, PTP IA-2 β, PTP κ, PTP μ, PTP ψ, PTP σ, PTP ζ, PTP-H1和PTP1B

PTP1B抗体（D-4）是一种IgG1 κ小鼠单克隆PTP1B抗体（也称为PTPN1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测人源PTP1B蛋白。PTP1B抗体（D-4）既可以是非偶联的抗PTP1B抗体形式，也可以是多种偶联形式的抗PTP1B抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。蛋白质酪氨酸残基的磷酸化早已被公认为是信号转导的重要调节组成部分。这是一个可逆的过程，涉及在酪氨酸残基上磷酸化蛋白质的酶以及快速扩展的蛋白质酪氨酸磷酸酶家族。后一类酶与蛋白质丝氨酸和蛋白质苏氨酸磷酸酶或酸性和碱性磷酸酶几乎没有相似之处。在大多数组织中，主要的PTP酶是一种对钒酸盐和钼酸盐敏感的蛋白质。基于序列分析，人胎盘中表达的PTP1B（PTPase 1B）与常见白细胞抗原（CD45）和神经粘附分子（NCAM）的同源物LAR具有相似性。PTP1B被合成为含有435个氨基酸的前体蛋白质，该前体蛋白质被切割以产生具有活性的321个氨基酸的酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PTP1B 抗体 (D-4) 参考文献:

  1. 克隆人类主要蛋白酪氨酸磷酸酶的 cDNA。  |  Chernoff, J., et al. 1990. Proc Natl Acad Sci U S A. 87: 2735-9. PMID: 2157211
  2. cdc2 的可逆酪氨酸磷酸化：进入有丝分裂过程中伴随着去磷酸化激活。  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
  3. 磷酸酪基蛋白磷酸酶。  |  Lau, KH., et al. 1989. Biochem J. 257: 23-36. PMID: 2537623
  4. 人类胎盘蛋白-酪氨酸磷酸酶：氨基酸序列及与受体样蛋白家族的关系。  |  Charbonneau, H., et al. 1989. Proc Natl Acad Sci U S A. 86: 5252-6. PMID: 2546149
  5. 裂殖酵母 cdc2+ 蛋白激酶的酪氨酸磷酸化调节有丝分裂的进入。  |  Gould, KL. and Nurse, P. 1989. Nature. 342: 39-45. PMID: 2682257
  6. 纯化人类胎盘的主要蛋白酪氨酸磷酸酶。  |  Tonks, NK., et al. 1988. J Biol Chem. 263: 6722-30. PMID: 2834386
  7. 免疫球蛋白超家族的新成员，其细胞质区域与白细胞共同抗原同源。  |  Streuli, M., et al. 1988. J Exp Med. 168: 1523-30. PMID: 2972792
  8. 蛋白酪氨酸激酶  |  Hunter, T. and Cooper, JA. 1985. Annu Rev Biochem. 54: 897-930. PMID: 2992362
  9. 通过破坏氧化还原复合物的形成来调节PTP1B的激活。  |  Londhe, AD., et al. 2020. Nat Chem Biol. 16: 122-125. PMID: 31873221
  10. PTP1B负调控巨噬细胞对铜绿假单胞菌的STAT1非依赖性杀伤。  |  Yue, L., et al. 2020. Biochem Biophys Res Commun. 533: 296-303. PMID: 32958258
  11. 蛋白酪氨酸磷酸酶1B（PTP1B）通过Src激活促进黑色素瘤细胞的进展。  |  Wang, Q., et al. 2021. Bioengineered. 12: 8396-8406. PMID: 34606417
  12. 通过高通量虚拟筛选发现新型PTP1B抑制剂。  |  Debnath, A., et al. 2024. Curr Comput Aided Drug Des.. PMID: 39411941

订购信息

产品名称产品编号规格价格数量收藏夹

PTP1B 抗体 (D-4)

sc-133259
200 µg/ml
RMB2377.00

PTP1B (D-4): m-IgG Fc BP-HRP 套装

sc-528786
200 µg Ab; 10 µg BP
RMB2662.00

PTP1B (D-4): m-IgGκ BP-HRP 套装

sc-521294
200 µg Ab, 40 µg BP
RMB2662.00

PTP1B (D-4): m-IgG1 BP-HRP 套装

sc-543129
200 µg Ab; 20 µg BP
RMB2662.00

PTP1B 抗体 (D-4) AC

sc-133259 AC
500 µg/ml, 25% agarose
RMB3129.00

PTP1B 抗体 (D-4) HRP

sc-133259 HRP
200 µg/ml
RMB2377.00

PTP1B 抗体 (D-4) FITC

sc-133259 FITC
200 µg/ml
RMB2482.00

PTP1B 抗体 (D-4) PE

sc-133259 PE
200 µg/ml
RMB2580.00

PTP1B 抗体 (D-4) Alexa Fluor® 488

sc-133259 AF488
200 µg/ml
RMB2685.00

PTP1B 抗体 (D-4) Alexa Fluor® 546

sc-133259 AF546
200 µg/ml
RMB2685.00

PTP1B 抗体 (D-4) Alexa Fluor® 594

sc-133259 AF594
200 µg/ml
RMB2685.00

PTP1B 抗体 (D-4) Alexa Fluor® 647

sc-133259 AF647
200 µg/ml
RMB2685.00

PTP1B 抗体 (D-4) Alexa Fluor® 680

sc-133259 AF680
200 µg/ml
RMB2685.00

PTP1B 抗体 (D-4) Alexa Fluor® 790

sc-133259 AF790
200 µg/ml
RMB2685.00

May I ask whether PTP1B (D-4): sc-133259 monoclonal antibody can be used to detect the expression of PTP1B in living tissue cells of mice?

Asked by: Zhao
Thank you for your question. PTP1B Antibody (D-4) is recommended for detection of PTP1B of human origin by WB, IP, IF, IHC(P) and ELISA. It wasn't verified on mouse sample.
Answered by: Technical Service SH
Date published: 2025-08-11

Ist der Antikörper reversibel oder irreversibel an die Agarose gebunden?

Asked by: Marienfeld
Vielen Dank für Ihre Anfrage und Ihr Interesse an diesem Produkt. Der als Agarose-Konjugat erhältliche PTP1B Antikörper (D-4): sc-133259 AC ist kovalent an Agarose-Beads über primäre Amine gebunden. Für weitere Informationen wenden Sie sich bitte an unseren Technischen Service in unserem Europäischen Supportbüro in Heidelberg. Sie erreichen uns telefonisch unter 06221 4503 0, per email an: [email protected] oder per Live-Chat auf unserer Webseite www.scbt.com Wir freuen uns Ihnen weiterzuhelfen.
Answered by: Technical Support Europe
Date published: 2017-05-22

Can PTP1B (D-4): sc-133259 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PTP1B (D-4): sc-133259 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IHCOur lab usually use this antibody staining PTP1B expression in Human liver cancer tissue section by IHC.We usuallly use goat serum for blocking and 1;100 dilution .We use ABC kit from Vector Labs.
Date published: 2018-05-26
Rated 4 out of 5 by from Very good results with western blotsvery good result (single sharp band with expected size) with HUVECS lysates.
Date published: 2018-01-26
Rated 4 out of 5 by from Fairly good!Although there is a non-specific band in some cell lysates, the signal is strong and clean.
Date published: 2017-11-15
Rated 5 out of 5 by from GreatI could detect one strong band using HEK293 whole cell lysate.
Date published: 2017-10-13
Rated 4 out of 5 by from Western blotI tested with PTP1B(D-4), SC133259 for Western blot in 293 cotransfection. This antibody works well.
Date published: 2017-05-10
Rated 3 out of 5 by from not worked in rat cellsI tried this antibody in L6 cells, but it not worked. No band can be detected.
Date published: 2017-05-10
Rated 4 out of 5 by from woking wellI used this antibody for Western blot with 293 cotransfected cell lysate. I can detect a clear thick band with expected size even though 293 cells express low amount of endogenous PTP1B.
Date published: 2017-04-20
Rated 5 out of 5 by from Strong and robutsPTP1B Antibody (D-4) produced strong and robust results by Western blot and IHCP
Date published: 2017-01-27
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PTP1B 抗体 (D-4) is rated 4.2 out of 5 by 13.
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