Date published: 2025-9-7
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PTN Antibody (H-6) - Western Blotting - Image 363123
PTN 抗体 (H-6): sc-74443. NCI-H460 (A), NTERA-2 cl. D1 (B) 和 NIH/3T3 (C) 全细胞裂解液中 PTN 表达的 Western 印迹分析.
PTN 抗体 (H-6): sc-74443. NCI-H460 (A), NTERA-2 cl. D1 (B) 和 NIH/3T3 (C) 全细胞裂解液中 PTN 表达的 Western 印迹分析.

PTN 抗体 (H-6): sc-74443

5.0(6)
寫評論提問

说明书
  • PTN 抗体 H-6 是小鼠单克隆 IgG1 κ，PTN抗体, 在17篇文献中引用，规格为200 µg/ml
  • 免疫human物种的PTN的氨基酸94-168
  • PTN 抗体 (H-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 precursor and mature PTN
  • 抗PTN抗体（H-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PTN (H-6): sc-74443的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 PTN 抗体 (H-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与PTN 抗体 (H-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

PTN抗体（H-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源PTN蛋白。抗PTN抗体（H-6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Pleiotrophin（PTN）是一种肝素结合生长因子，在细胞增殖、分化、迁移等细胞过程中发挥着关键作用，因此PTN在发育生物学和组织修复中具有重要意义。PTN蛋白由168个氨基酸组成，可被切割成136个氨基酸的活性形式，与midkine（MK）的相似度约为50%，并保留了所有10个半胱氨酸残基，这些残基对于结构完整性和功能至关重要。PTN主要在各种细胞类型中表达，包括成骨细胞、软骨细胞和神经元，在胎儿发育过程中尤为重要，PTN支持神经组织的生长和分化。在成年生物中，PTN的表达主要局限于大脑，这凸显了PTN在神经发育中的特殊作用以及在神经退行性疾病中的潜在影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PTN 抗体 (H-6) 参考文献:

  1. 新生长因子褶皱素（HB-GAM）mRNA 在发育中和成年大鼠组织中的细胞分布。  |  Vanderwinden, JM., et al. 1992. Anat Embryol (Berl). 186: 387-406. PMID: 1416088
  2. 人类褶皱素基因的克隆, 核苷酸序列和染色体定位。  |  Milner, PG., et al. 1992. Biochemistry. 31: 12023-8. PMID: 1457401
  3. 人类褶皱素基因的特征。启动子区域和染色体定位。  |  Li, YS., et al. 1992. J Biol Chem. 267: 26011-6. PMID: 1464612
  4. 胎鼠, 新生鼠和成年鼠脑中 HBNF（肝素结合神经元促进因子）基因的表达：原位杂交研究。  |  Bloch, B., et al. 1992. Brain Res Dev Brain Res. 70: 267-78. PMID: 1477961
  5. HBNF 和 MK，肝素结合蛋白新型基因家族的成员，在胚胎发育和大脑功能中具有潜在作用。  |  Böhlen, P. and Kovesdi, I. 1991. Prog Growth Factor Res. 3: 143-57. PMID: 1773041
  6. 一种受小鸡胚胎视黄酸调控的新型肝素结合蛋白  |  Raulais, D., et al. 1991. Biochem Biophys Res Commun. 174: 708-15. PMID: 1993066
  7. 克隆并表达一种诱导有丝分裂和神经元生长活性的发育调控蛋白。  |  Li, YS., et al. 1990. Science. 250: 1690-4. PMID: 2270483
  8. 周皮脱落会导致循环衰竭和多营养素耗竭，造成神经元缺失。  |  Nikolakopoulou, AM., et al. 2019. Nat Neurosci. 22: 1089-1098. PMID: 31235908
  9. Sec13通过自分泌多营养素信号促进少突胶质细胞分化和髓鞘修复。  |  Liu, Z., et al. 2022. J Clin Invest. 132: PMID: 35143418
  10. Pleiotrophin能改善年龄诱导的成人海马神经发生衰退和认知功能障碍。  |  Li, H., et al. 2023. Cell Rep. 42: 113022. PMID: 37610873
  11. Pleiotrophin 能转化 NIH 3T3 细胞并诱导裸鼠患肿瘤。  |  Chauhan, AK., et al. 1993. Proc Natl Acad Sci U S A. 90: 679-82. PMID: 8421705
  12. 一种新型肝素结合生长因子--pleiotrophin在大鼠神经系统中的表达受发育调控。  |  Wanaka, A., et al. 1993. Brain Res Dev Brain Res. 72: 133-44. PMID: 8453763

订购信息

产品名称产品编号规格价格数量收藏夹

PTN 抗体 (H-6)

sc-74443
200 µg/ml
RMB2377.00

PTN (H-6): m-IgG Fc BP-HRP 套装

sc-525711
200 µg Ab; 10 µg BP
RMB2662.00

PTN 抗体 (H-6) AC

sc-74443 AC
500 µg/ml, 25% agarose
RMB3129.00

PTN 抗体 (H-6) HRP

sc-74443 HRP
200 µg/ml
RMB2377.00

PTN 抗体 (H-6) FITC

sc-74443 FITC
200 µg/ml
RMB2482.00

PTN 抗体 (H-6) PE

sc-74443 PE
200 µg/ml
RMB2580.00

PTN 抗体 (H-6) Alexa Fluor® 488

sc-74443 AF488
200 µg/ml
RMB2685.00

PTN 抗体 (H-6) Alexa Fluor® 546

sc-74443 AF546
200 µg/ml
RMB2685.00

PTN 抗体 (H-6) Alexa Fluor® 594

sc-74443 AF594
200 µg/ml
RMB2685.00

PTN 抗体 (H-6) Alexa Fluor® 647

sc-74443 AF647
200 µg/ml
RMB2685.00

PTN 抗体 (H-6) Alexa Fluor® 680

sc-74443 AF680
200 µg/ml
RMB2685.00

PTN 抗体 (H-6) Alexa Fluor® 790

sc-74443 AF790
200 µg/ml
RMB2685.00

Hi I am using this antibody for western blot in NT2 cells and I detect two bands around molecular weight 25KDa and 17KDa. I also noticed you have shown the western blot image of PTN in NT2 cells. Could you help us verify which is the correct PTN band.

Asked by: Charan
Thank you for your question. PTN has a predicted molecular weight of ~19 kDa, however we typically observe PTN in cell lines between 18-25 kDa. The Western blot data published on our website is representative of what we observe using the antibody in cell lines tested. We recommend you include positive and negative controls in your evaluation of the antibody in your samples to evaluate the specificity of your results. Please contact our Technical Service Department for support in troubleshooting the antibody.
Answered by: Technical Support
Date published: 2019-06-07

I am using PTN (H-6): sc-74443 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Okay antibodyDoes not work well with immunostaining in brain tissue
Date published: 2025-03-15
Rated 5 out of 5 by from Great choice for WBProduced robust, clean WB bands in CI-H460, NTERA-2 cl.D1 and NIH/3T3 whole cell lysates.
Date published: 2017-01-30
Rated 5 out of 5 by from Published IF data using Human amniotic epithelialPublished IF data using Human amniotic epithelial cells. -SCBT Publication Review
Date published: 2015-06-18
Rated 5 out of 5 by from Robust signal in western blot analysis ofRobust signal in western blot analysis of human recombinant PTN under reducing conditions. -SCBT QC
Date published: 2015-06-15
Rated 5 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by from Great immunoperoxidase membrane stainingGreat immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human parathyroid gland tissue. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Published immunohistochemical analysis usingPublished immunohistochemical analysis using paraffin embedded endothelial cells. -SCBT Publication Review
Date published: 2015-01-12
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PTN 抗体 (H-6) is rated 4.7 out of 5 by 7.
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